Anti-LASP1 antibody - N-terminal (ab191022)
Key features and details
- Rabbit polyclonal to LASP1 - N-terminal
- Suitable for: IHC-P, ICC, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-LASP1 antibody - N-terminal
See all LASP1 primary antibodies -
Description
Rabbit polyclonal to LASP1 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Rabbit, Cow, Orangutan -
Immunogen
Synthetic peptide corresponding to Human LASP1 aa 1-100 (N terminal).
Database link: Q14847 -
Positive control
- Rat liver, spleen and intestine tissue lysate. Jurkat, MCF7, A431, HeLa and 293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC |
Use a concentration of 0.5 - 1 µg/ml.
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 30 kDa.
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Notes |
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC
Use a concentration of 0.5 - 1 µg/ml. |
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 30 kDa. |
Target
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Function
Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types. -
Sequence similarities
Contains 1 LIM zinc-binding domain.
Contains 2 nebulin repeats.
Contains 1 SH3 domain. -
Cellular localization
Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Associated with the F-actin rich cortical cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 532851 Cow
- Entrez Gene: 3927 Human
- Entrez Gene: 100173670 Orangutan
- Entrez Gene: 100009568 Rabbit
- Entrez Gene: 29278 Rat
- Omim: 602920 Human
- SwissProt: Q3B7M5 Cow
- SwissProt: Q14847 Human
see all -
Alternative names
- LASP 1 antibody
- LASP-1 antibody
- LASP1 antibody
see all
Images
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All lanes : Anti-LASP1 antibody - N-terminal (ab191022) at 0.5 µg/ml
Lane 1 : Rat liver tissue lysate
Lane 2 : Rat spleen tissue lysate
Lane 3 : Rat intestine tissue lysate
Lane 4 : Jurkat cell lysate
Lane 5 : MCF7 cell lysate
Lane 6 : A431 cell lysate
Lane 7 : HeLa cell lysate
Lane 8 : 293T cell lysate
Predicted band size: 30 kDa -
Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human lung cancer tissue labelling LASP1 with ab191022 at 1 µg/ml.
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Immunocytochemical analysis of A549 cells, staining LASP1 with ab191022 at 1 µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab191022 has been referenced in 7 publications.
- Zhang Z et al. miR-218-5p in endometrial microenvironment prevents the migration of ectopic endometrial stromal cells by inhibiting LASP1. Reprod Biol Endocrinol 20:64 (2022). PubMed: 35379225
- Bai L et al. Circ_0001535 Facilitates Tumor Malignant Progression by miR-485-5p/LASP1 Axis in Colorectal Cancer. Balkan Med J 39:411-421 (2022). PubMed: 36172827
- Liu F et al. Endometrial stromal cell proteomic analysis reveals LIM and SH3 protein 1 (LASP1) plays important roles in the progression of adenomyosis. Mol Hum Reprod 27:N/A (2021). PubMed: 33543750
- Chen N et al. LASP1 induces colorectal cancer proliferation and invasiveness through Hippo signaling and Nanog mediated EMT. Am J Transl Res 12:6490-6500 (2020). PubMed: 33194046
- Yang LX et al. Suppression of long non-coding RNA TNRC6C-AS1 protects against thyroid carcinoma through DNA demethylation of STK4 via the Hippo signalling pathway. Cell Prolif 52:e12564 (2019). PubMed: 30938030
- Zhang X et al. Lasp1 promotes malignant phenotype of non-small-cell lung cancer via inducing phosphorylation of FAK-AKT pathway. Oncotarget 8:75102-75113 (2017). PubMed: 29088849
- Andrews MC et al. Systems analysis identifies miR-29b regulation of invasiveness in melanoma. Mol Cancer 15:72 (2016). WB ; Human . PubMed: 27852308