Key features and details
- Rabbit polyclonal to LASP1 - N-terminal
- Suitable for: IHC-P, ICC, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-LASP1 antibody - N-terminal
See all LASP1 primary antibodies
DescriptionRabbit polyclonal to LASP1 - N-terminal
SpecificityNo cross reactivity with other proteins.
Tested applicationsSuitable for: IHC-P, ICC, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Cow, Orangutan
- Rat liver, spleen and intestine tissue lysate. Jurkat, MCF7, A431, HeLa and 293T cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab191022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC||Use a concentration of 0.5 - 1 µg/ml.|
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 30 kDa.|
FunctionPlays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types.
Sequence similaritiesContains 1 LIM zinc-binding domain.
Contains 2 nebulin repeats.
Contains 1 SH3 domain.
Cellular localizationCytoplasm > cell cortex. Cytoplasm > cytoskeleton. Associated with the F-actin rich cortical cytoskeleton.
- Information by UniProt
- LASP 1 antibody
- LASP-1 antibody
- LASP1 antibody
All lanes : Anti-LASP1 antibody - N-terminal (ab191022) at 0.5 µg/ml
Lane 1 : Rat liver tissue lysate
Lane 2 : Rat spleen tissue lysate
Lane 3 : Rat intestine tissue lysate
Lane 4 : Jurkat cell lysate
Lane 5 : MCF7 cell lysate
Lane 6 : A431 cell lysate
Lane 7 : HeLa cell lysate
Lane 8 : 293T cell lysate
Predicted band size: 30 kDa
Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human lung cancer tissue labelling LASP1 with ab191022 at 1 µg/ml.
Immunocytochemical analysis of A549 cells, staining LASP1 with ab191022 at 1 µg/ml.
ab191022 has been referenced in 3 publications.
- Yang LX et al. Suppression of long non-coding RNA TNRC6C-AS1 protects against thyroid carcinoma through DNA demethylation of STK4 via the Hippo signalling pathway. Cell Prolif 52:e12564 (2019). PubMed: 30938030
- Zhang X et al. Lasp1 promotes malignant phenotype of non-small-cell lung cancer via inducing phosphorylation of FAK-AKT pathway. Oncotarget 8:75102-75113 (2017). PubMed: 29088849
- Andrews MC et al. Systems analysis identifies miR-29b regulation of invasiveness in melanoma. Mol Cancer 15:72 (2016). WB ; Human . PubMed: 27852308