Product nameAnti-LASP1 antibody - N-terminal
See all LASP1 primary antibodies
DescriptionRabbit polyclonal to LASP1 - N-terminal
SpecificityNo cross reactivity with other proteins.
Tested applicationsSuitable for: IHC-P, ICC, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Cow, Orangutan
- Rat liver, spleen and intestine tissue lysate. Jurkat, MCF7, A431, HeLa and 293T cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab191022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC||Use a concentration of 0.5 - 1 µg/ml.|
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 30 kDa.|
FunctionPlays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types.
Sequence similaritiesContains 1 LIM zinc-binding domain.
Contains 2 nebulin repeats.
Contains 1 SH3 domain.
Cellular localizationCytoplasm > cell cortex. Cytoplasm > cytoskeleton. Associated with the F-actin rich cortical cytoskeleton.
- Information by UniProt
- LASP 1 antibody
- LASP-1 antibody
- LASP1 antibody
All lanes : Anti-LASP1 antibody - N-terminal (ab191022) at 0.5 µg/ml
Lane 1 : Rat liver tissue lysate
Lane 2 : Rat spleen tissue lysate
Lane 3 : Rat intestine tissue lysate
Lane 4 : Jurkat cell lysate
Lane 5 : MCF7 cell lysate
Lane 6 : A431 cell lysate
Lane 7 : HeLa cell lysate
Lane 8 : 293T cell lysate
Predicted band size: 30 kDa
Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human lung cancer tissue labelling LASP1 with ab191022 at 1 µg/ml.
Immunocytochemical analysis of A549 cells, staining LASP1 with ab191022 at 1 µg/ml.
This product has been referenced in:
- Zhang X et al. Lasp1 promotes malignant phenotype of non-small-cell lung cancer via inducing phosphorylation of FAK-AKT pathway. Oncotarget 8:75102-75113 (2017). Read more (PubMed: 29088849) »
- Andrews MC et al. Systems analysis identifies miR-29b regulation of invasiveness in melanoma. Mol Cancer 15:72 (2016). WB ; Human . Read more (PubMed: 27852308) »