Overview

  • Product name

    Anti-LASP1 antibody - N-terminal
    See all LASP1 primary antibodies
  • Description

    Rabbit polyclonal to LASP1 - N-terminal
  • Host species

    Rabbit
  • Specificity

    No cross reactivity with other proteins.
  • Tested applications

    Suitable for: IHC-P, ICC, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Cow, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human LASP1 aa 16-30 (N terminal).
    Sequence:

    EKVNCLDKFWHKACF


    Database link: Q14847

  • Positive control

    • Rat liver, spleen and intestine tissue lysate. Jurkat, MCF7, A431, HeLa and 293T cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 30 kDa.

Target

Images

  • All lanes : Anti-LASP1 antibody - N-terminal (ab191022) at 0.5 µg/ml

    Lane 1 : Rat liver tissue lysate
    Lane 2 : Rat spleen tissue lysate
    Lane 3 : Rat intestine tissue lysate
    Lane 4 : Jurkat cell lysate
    Lane 5 : MCF7 cell lysate
    Lane 6 : A431 cell lysate
    Lane 7 : HeLa cell lysate
    Lane 8 : 293T cell lysate

    Predicted band size: 30 kDa

  • Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human lung cancer tissue labelling LASP1 with ab191022 at 1 µg/ml.

  • Immunocytochemical analysis of A549 cells, staining LASP1 with ab191022 at 1 µg/ml.

References

This product has been referenced in:

  • Zhang X  et al. Lasp1 promotes malignant phenotype of non-small-cell lung cancer via inducing phosphorylation of FAK-AKT pathway. Oncotarget 8:75102-75113 (2017). Read more (PubMed: 29088849) »
  • Andrews MC  et al. Systems analysis identifies miR-29b regulation of invasiveness in melanoma. Mol Cancer 15:72 (2016). WB ; Human . Read more (PubMed: 27852308) »
See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab191022.
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