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    lasv-gp-antibody-ab190655.pdf

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Microbiology Organism Virus RNA Virus single stranded RNA negative strand virus Other Viruses
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Anti-LASV GP antibody (ab190655)

  • Datasheet
  • SDS
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Western blot - Anti-LASV GP antibody (ab190655)

    Key features and details

    • Rabbit polyclonal to LASV GP
    • Suitable for: WB
    • Reacts with: Species independent
    • Isotype: IgG

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    Overview

    • Product name

      Anti-LASV GP antibody
    • Description

      Rabbit polyclonal to LASV GP
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Species independent
    • Immunogen

      Synthetic peptide corresponding to LASV GP.
      Database link: P08669

    • Positive control

      • Recombinant Lassa virus glycoprotein (LASV GP).
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.02% Sodium azide
      Constituent: 99% PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Microbiology
      • Organism
      • Virus
      • RNA Virus
      • single stranded RNA negative strand virus
      • Other Viruses
      • Microbiology
      • Protein
      • Viral Protein

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab190655 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    Use a concentration of 0.1 µg/ml. Predicted molecular weight: 56 kDa.
    Notes
    WB
    Use a concentration of 0.1 µg/ml. Predicted molecular weight: 56 kDa.

    Target

    • Cellular localization

      Host cell membrane; Host membrane; Viral envelope protein; Virion.
    • Alternative names

      • glycoprotein antibody

    Images

    • Western blot - Anti-LASV GP antibody (ab190655)
      Western blot - Anti-LASV GP antibody (ab190655)
      All lanes : Anti-LASV GP antibody (ab190655) at 0.1 µg/ml

      Lane 1 : Recombinant Lassa virus glycoprotein at 0.05 µg
      Lane 2 : Recombinant Lassa virus glycoprotein at 0.1 µg

      Secondary
      All lanes : Anti-rabbit IgG-HRP conjugate and TMB membrane substrate

      Performed under reducing conditions.

      Predicted band size: 56 kDa
      Observed band size: 60 kDa why is the actual band size different from the predicted?

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab190655? Please let us know so that we can cite the reference in this datasheet.

    ab190655 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Filter by Application

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    Western blot abreview for Anti-LASV GP antibody

    Below Average
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    African Green Monkey Recombinant protein (VERO cell supernatant)
    Gel Running Conditions
    Reduced Denaturing (4-15% TGX (BioRad) gel)
    Loading amount
    10 µg
    Specification
    VERO cell supernatant
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C
    Read More

    Sally Boyd

    Verified customer

    Submitted Aug 14 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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