Overview

  • Product name

  • Description

    Rabbit polyclonal to LAT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide from the C terminus of Human LAT (NP_001014987.1), conjugated to KLH.

  • Positive control

    • WB: MCF7 cell lysate IHC-P: Human breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab86751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 28 kDa.
IHC-P 1/50 - 1/100.

Target

  • Function

    Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
  • Tissue specificity

    Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
  • Post-translational
    modifications

    Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ.
    Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.
  • Cellular localization

    Cell membrane. Present in lipid rafts.
  • Information by UniProt
  • Database links

  • Alternative names

    • 36 kDa phospho tyrosine adapter protein antibody
    • 36 kDa phospho-tyrosine adapter protein antibody
    • 36 kDa phospho-tyrosine adaptor protein antibody
    • LAT 1 antibody
    • lat antibody
    • LAT_HUMAN antibody
    • LAT1 antibody
    • Linker for activation of T cells antibody
    • Linker for activation of T cells family member 1 antibody
    • linker for activation of T cells, transmembrane adaptor antibody
    • Linker for activation of T-cells family member 1 antibody
    • p36 38 antibody
    • p36-38 antibody
    • pp36 antibody
    see all

Images

  • Anti-LAT antibody (ab86751) at 1/100 dilution + MCF7 cell lysate at 35 µg

    Predicted band size: 28 kDa

  • Formalin fixed and paraffin embedded Human breast carcinoma tissue labelled with a 1/50 dilution of ab86751, which was peroxidase conjugated to the secondary antibody, followed by DAB staining.

References

ab86751 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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