• Product name

    Anti-LAT (phospho Y191) antibody
    See all LAT primary antibodies
  • Description

    Rabbit polyclonal to LAT (phospho Y191)
  • Host species

  • Specificity

    Detects endogenous levels of LAT only when phosphorylated at tyrosine 191. This antibody detects LAT phosphorylated on Tyrosine 191 in isoform 2 (SwissProt: O43561-2), and Tyrosine 220 in isoform 1 (SwissProt: O43561).
  • Tested applications

    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic phosphopeptide from around the phosphorylation site of tyrosine 191 (REYPVN) of human LAT

  • Positive control

    • Human colon carcinoma tissue NIH3T3 cell extract



Our Abpromise guarantee covers the use of ab59197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.


  • Function

    Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
  • Tissue specificity

    Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
  • Post-translational

    Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ.
    Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.
  • Cellular localization

    Cell membrane. Present in lipid rafts.
  • Information by UniProt
  • Database links

  • Alternative names

    • 36 kDa phospho tyrosine adapter protein antibody
    • 36 kDa phospho-tyrosine adapter protein antibody
    • 36 kDa phospho-tyrosine adaptor protein antibody
    • LAT 1 antibody
    • lat antibody
    • LAT_HUMAN antibody
    • LAT1 antibody
    • Linker for activation of T cells antibody
    • Linker for activation of T cells family member 1 antibody
    • linker for activation of T cells, transmembrane adaptor antibody
    • Linker for activation of T-cells family member 1 antibody
    • p36 38 antibody
    • p36-38 antibody
    • pp36 antibody
    see all


  • Immunohistochemical analysis of paraffin embedded human colon carcinoma tissue using ab59197 at 1/50 dilution. Left: untreated. Right: sample treated with immunising peptide.
  • All lanes : Anti-LAT (phospho Y191) antibody (ab59197) at 1/500 dilution

    Lane 1 : NIH3T3 cells treated with PMA
    Lane 2 : NIH3T3 cells treated with PMA with phosphopeptide

    Predicted band size: 28 kDa
    Observed band size: 28 kDa


This product has been referenced in:

  • Bustos-Morán E  et al. Microtubule-associated protein-4 controls nanovesicle dynamics and T cell activation. J Cell Sci 130:1217-1223 (2017). Read more (PubMed: 28209780) »
  • Crites TJ  et al. TCR Microclusters pre-exist and contain molecules necessary for TCR signal transduction. J Immunol 193:56-67 (2014). WB ; Mouse . Read more (PubMed: 24860189) »
See all 3 Publications for this product

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