Product nameAnti-LAT (phospho Y191) antibody
See all LAT primary antibodies
DescriptionRabbit polyclonal to LAT (phospho Y191)
SpecificityDetects endogenous levels of LAT only when phosphorylated at tyrosine 191. This antibody detects LAT phosphorylated on Tyrosine 191 in isoform 2 (SwissProt: O43561-2), and Tyrosine 220 in isoform 1 (SwissProt: O43561).
Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic phosphopeptide from around the phosphorylation site of tyrosine 191 (REYPVN) of human LAT
- Human colon carcinoma tissue NIH3T3 cell extract
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab59197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).|
|IHC-P||Use at an assay dependent concentration.|
FunctionRequired for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
Tissue specificityExpressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
modificationsPhosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ.
Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.
Cellular localizationCell membrane. Present in lipid rafts.
- Information by UniProt
- 36 kDa phospho tyrosine adapter protein antibody
- 36 kDa phospho-tyrosine adapter protein antibody
- 36 kDa phospho-tyrosine adaptor protein antibody
Immunohistochemical analysis of paraffin embedded human colon carcinoma tissue using ab59197 at 1/50 dilution. Left: untreated. Right: sample treated with immunising peptide.
All lanes : Anti-LAT (phospho Y191) antibody (ab59197) at 1/500 dilution
Lane 1 : NIH3T3 cells treated with PMA
Lane 2 : NIH3T3 cells treated with PMA with phosphopeptide
Predicted band size: 28 kDa
Observed band size: 28 kDa
This product has been referenced in:
- Bustos-Morán E et al. Microtubule-associated protein-4 controls nanovesicle dynamics and T cell activation. J Cell Sci 130:1217-1223 (2017). Read more (PubMed: 28209780) »
- Crites TJ et al. TCR Microclusters pre-exist and contain molecules necessary for TCR signal transduction. J Immunol 193:56-67 (2014). WB ; Mouse . Read more (PubMed: 24860189) »