Key features and details
- Rabbit polyclonal to LAT2
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-LAT2 antibody
See all LAT2 primary antibodies
DescriptionRabbit polyclonal to LAT2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat
Recombinant fragment (His-T7-tag) corresponding to Rat LAT2 aa 32-192. Expressed in E.coli. N-terminal tags.
RPGVKRNEKIYEQRNQQENEQSVASSQTYSLARPVRTGLQMDAASNKSFE RKNKLLFSHLEGPESPRYQNFYKGSRRESDAAYVDPIPTDYYNWGCFQKP PEDNDSNSYENVLICKPSTPESGTEESEDYQNSVSILQWRESKRTMGART SPSGSPDEEPD
Database link: Q9WVR6
- IHC-P: Rat spleen tissue. WB: Rat spleen lysate; Recombinant rat LAT2 protein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab233088 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233088 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 58 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionSodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney.
Tissue specificityStrongest expression is observed in kidney and moderate expression in placenta and brain, followed by liver, prostate, testis, ovary, lymph node, thymus, spleen, skeletal muscle and heart. Also expressed in fetal liver as well as in the retinal pigment epithelial cell line ARPE-19 and the intestinal epithelial cell line Caco-2.
Sequence similaritiesBelongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.
Cellular localizationCytoplasm. Basolateral cell membrane. Localized to the cytoplasm when expressed alone but when coexpressed with SLC3A2/4F2hc, is localized to the plasma membrane. Colocalized with SLC3A2/4F2hc at the basolateral membrane of kidney cortex proximal tubules and small intestine epithelia of the villi.
- Information by UniProt
- hLAT2 antibody
- integral membrane protein E16H antibody
- L type amino acid transporter 2 antibody
Formalin-fixed, paraffin-embedded rat spleen tissue stained for LAT2 using ab233088 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Anti-LAT2 antibody (ab233088) at 2 µg/ml + Rat spleen lysate
HRP-Linked Goat Anti-Rabbit IgG at 0.2 µg/ml
Predicted band size: 58 kDa
Anti-LAT2 antibody (ab233088) at 2 µg/ml + Recombinant rat LAT2 protein
Predicted band size: 58 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233088 has not yet been referenced specifically in any publications.