Overview

  • Product name

  • Description

    Rabbit polyclonal to LAT2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human LAT2 aa 200-300 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab90595)

  • Positive control

    • This antibody gave a positive signal in the following lysates Human Liver Tissue; Mouse Kidney Tissue; Rat Kidney Tissue; Mouse Brain Tissue; HepG2 whole cell; HeLa whole cell.

Properties

Applications

Our Abpromise guarantee covers the use of ab75610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).

Target

  • Function

    Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney.
  • Tissue specificity

    Strongest expression is observed in kidney and moderate expression in placenta and brain, followed by liver, prostate, testis, ovary, lymph node, thymus, spleen, skeletal muscle and heart. Also expressed in fetal liver as well as in the retinal pigment epithelial cell line ARPE-19 and the intestinal epithelial cell line Caco-2.
  • Sequence similarities

    Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.
  • Cellular localization

    Cytoplasm. Basolateral cell membrane. Localized to the cytoplasm when expressed alone but when coexpressed with SLC3A2/4F2hc, is localized to the plasma membrane. Colocalized with SLC3A2/4F2hc at the basolateral membrane of kidney cortex proximal tubules and small intestine epithelia of the villi.
  • Information by UniProt
  • Database links

  • Alternative names

    • hLAT2 antibody
    • integral membrane protein E16H antibody
    • L type amino acid transporter 2 antibody
    • L-type amino acid transporter 2 antibody
    • Large neutral amino acids transporter small subunit 2 antibody
    • LAT2_HUMAN antibody
    • LPI PC1 antibody
    • SLC7A8 antibody
    • solute carrier family 7 (amino acid transporter light chain, L system), member 8 antibody
    • solute carrier family 7 (amino acid transporter, L type), member 8 antibody
    • solute carrier family 7 (cationic amino acid transporter, y+ system), member 8 antibody
    • Solute carrier family 7 member 8 antibody
    see all

Images

  • All lanes : Anti-LAT2 antibody (ab75610) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 58 kDa
    Observed band size: 58 kDa
    Additional bands at: 52 kDa, 85 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes
  • All lanes : Anti-LAT2 antibody (ab75610) at 1/1 dilution

    Lane 1 : Brain (Mouse) Tissue Lysate

    Lane 2 : Kidney (Rat) Tissue Lysate


    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 58 kDa
    Observed band size: 65 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 minutes

References

This product has been referenced in:

  • Dai W  et al. Understanding the regulatory mechanisms of milk production using integrative transcriptomic and proteomic analyses: improving inefficient utilization of crop by-products as forage in dairy industry. BMC Genomics 19:403 (2018). Read more (PubMed: 29843597) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Vielen Dank für Ihre Anfrage.


Gerne bin ich Ihnen bei der Wahl eines passenden Antikörpers behilflich. Für welche Spezies suchen Sie den den Antikörper?

Ich freue mich auf Ihre Antwort.

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Answer

Thank you very much for your interest in ab75610. To our knowledge, ab75610 has not been tested in IF. Therefore, I can offer a discount off a future purchase if you buy ab75610 now, test it in IF and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab75610 in IF. I will then send a discount code. This code must be issued before purchasing ab75610 so please wait for my reply before ordering. 2. Purchase ab75610 either by phone, fax, or online (www.abcam.com). 3. Test it in IF. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY  ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab75610 turns out to be unsuitable for IF, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.  

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