Latex Conjugation Kit - 400nm Blue (ab269892)
Overview
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Product name
Latex Conjugation Kit - 400nm Blue
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Product overview
Latex Conjugation Kit – 400nm Blue (ab269892) allows antibodies or proteins to be conjugated to our high quality 400 nm latex nanoparticles quickly and easily. The latex nanoparticles are designed for ease of use and have much improved handling compared with traditional latex. This has been achieved by specially treating the nanoparticles. The conjugation is covalent, and requires less antibody than traditional passive and covalent latex conjugation.
The 400 nm blue latex nanoparticles in this kit are freeze dried. The conjugation reaction is initiated simply by reconstituting the dry mixture with the antibody, which becomes attached (via lysine residues) to the specially treated surface.
It takes 30 seconds to set up the conjugation, the hands-on time for the conjugation procedure is about 3 minutes and the conjugate is ready to use within 35 minutes. The biomolecule is simply pipetted into a vial containing the latex nanoparticles, then a centrifugation allows a buffer exchange.
The resulting covalent conjugates can be easily resuspended without the need for harsh methods such as sonication or vortexing, unlike traditional conjugation procedures which are prone to aggregation. This is due to the properties of the surface treatment which makes the particles resistant to aggregation.
Additionally, unlike passive methods, the conjugation procedure has only a weak dependence on the isoelectric point of the antibody. Consequently, extensive trials at different pH values are not required; all antibodies can be conjugated at one of two pHs, both of which are supplied in this kit (Reaction Buffers A and B).
Benefits of Latex Conjugation Kit:
Only 30 seconds to set up the conjugation
Three minutes hands-on time for the conjugation procedure
Conjugate is ready to use within 35 minutes – the biomolecule is simply pipetted into a vial containing the latex nanoparticles, then a centrifugation allows a buffer exchange.
General notes:
This product is manufactured by Expedeon, an Abcam company. It was previously called 400nm Blue Latex Conjugation Kit. Expedeon product code 1000-0040 (4 reaction mini kit) is the same as the 4 x 4 µg size of this product, code 1000-0100 (10 reaction mini kit) is the same as the 10 x 4 µg size, and code 1000-0120 (1 reaction mini kit) is the same as the 1 x 40 µg size of this product.
Buffer requirements:
We would suggest that the antibody to be conjugated should be in 10 – 50 mM MES, HEPES or MOPS at pH 6 – 7 (with no other components e.g. salt or azide) before conjugation. For incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits for Nanoparticles. To learn more about incompatible buffers, please refer to the protocol booklet.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 4 x 4 µg 10 x 4 µg 1 x 40 µg ab273906 - 400 nm Blue Latex vial (Lyophilized) 4 x 4µg 10 x 4µg 1 x 40µg ab273904 - Quencher (10X) 1 x 1500µl 1 x 1500µl 1 x 1500µl ab273902 - Reaction Buffer A (1X) 1 x 1ml 1 x 1ml 1 x 1ml ab273903 - Reaction Buffer B (1X) 1 x 1ml 1 x 1ml 1 x 1ml ab273905 - Resuspension Buffer 1 x 1ml 1 x 1ml 1 x 1ml
Images
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Bond, Meaghan, et al used Latex Conjugation Kit - 400nm Blue (ab269892) as part of examining rapid tests to identify patients with sickle cell disease. They used the kit to conjugate Latex to anti-HbS and anti-HbA antibodies for use in lateral flow assay.
(a) Representative strips run with varying amounts of normal blood, including a negative control with no blood. Top image shows full-color scan; bottom image shows red channel of the same image. (b) Signal-to-background ratio of strips in part a (normal blood) and the A threshold set by previous experiment. Error bars represent ±1 SD of three strips. *10 µL represents the average of only 2 strips. (c) Representative strips run with varying amounts of sickle cell anemia (SCA) blood, including a negative control with no blood. (d) Signal-to-background ratio of strips in part c (SCA blood) and the S threshold set by previous experiment. A line present at the "A" line indicates normal, at the "S" line indicates SCA, and at neither indicates sickle cell trait (SCT). The positive control line must be present for the test to be valid. -
Kim, Jinsu, et al used Latex Conjugation Kit - 400nm Blue (ab269892) as part of examining human malaria species with multiplex lateral flow immunoassay. They used the kit to conjugate Latex to anti-(pan)pLDH antibodies for use in lateral flow assay.
Representative test strips of the two-colour LFA. Distinct colours were observed at the test lines, corresponding a type of antigens, such as a blue test lines for pLDH detection only, b mixture colour of test lines for simultaneous detection, and c red test lines for PfHRP2 detection only. The mixture colour at control lines indicated the red and blue latex particles migrated along the length of the strip -
Rutkowska, Aleksandra, et al used Latex Conjugation Kit – 400nm Blue (ab269892) as part of developing a novel method for rapid and quantitative detection of bisphenol A (BPA) in urine. They used the kit to conjugate Latex to polyclonal anti-Bisphenol A antibodies for use in lateral flow assay.
(A) A scheme of using lateral flow assay for detection of BPA in urine samples. (B) Determination of BPA concentration in urine samples by lateral flow strips and estimation of different levels of individual exposure. (C) Examples of results of the lateral flow assay for differ-ent BPA concentrations in the urine samples, with results validated by LC-MS/MS. -
The three colours of latex each conjugated to a different antibody or protein, forming a line either by a direct binding event (red and black latex) or a sandwich assay binding event (blue latex). The three colours of latex demonstrate no aggregation or background staining.
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Lateral Flow Assay of our specially treated latex beads conjugated to a monoclonal anti-CRP antibody (mAb1) titrated against CRP. 2ng/mL CRP can easily be detected.
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Lateral Flow Assay of our specially treated latex conjugated to the monoclonal anti-CRP antibody (mAb2) titrated against CRP in buffer or CRP spiked CRP depleted serum (100 % serum). The latex behaves similarly in serum and buffer, and no aggregation or nonspecific binding is seen.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab269892 has been referenced in 6 publications.
- Rutkowska A et al. A novel method for rapid and quantitative detection of bisphenol A in urine. Acta Biochim Pol 67:409-415 (2020). PubMed: 32730702
- Wang R et al. Rapid Diagnostic Platform for Colorimetric Differential Detection of Dengue and Chikungunya Viral Infections. Anal Chem 91:5415-5423 (2019). PubMed: 30896928
- Kim J et al. A two-colour multiplexed lateral flow immunoassay system to differentially detect human malaria species on a single test line. Malar J 18:313 (2019). PubMed: 31533756
- Budama-Kilinc Y et al. Production and characterization of a conserved M2e peptide-based specific IgY antibody: evaluation of the diagnostic potential via conjugation with latex nanoparticles. Prep Biochem Biotechnol 48:930-939 (2018). PubMed: 30388960
- Bond M et al. Towards a point-of-care strip test to diagnose sickle cell anemia. PLoS One 12:e0177732 (2017). PubMed: 28520780
- Lee S et al. Two-Color Lateral Flow Assay for Multiplex Detection of Causative Agents Behind Acute Febrile Illnesses. Anal Chem 88:8359-63 (2016). PubMed: 27490379