Product nameLatex Conjugation Kit – 400nm Blue
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Latex Conjugation Kit – 400nm Blue (ab269892) allows antibodies or proteins to be conjugated to our high quality 400 nm latex nanoparticles quickly and easily. The latex nanoparticles are designed for ease of use and have much improved handling compared with traditional latex. This has been achieved by specially treating the nanoparticles. The conjugation is covalent, and requires less antibody than traditional passive and covalent latex conjugation.
The 400 nm blue latex nanoparticles in this kit are freeze dried. The conjugation reaction is initiated simply by reconstituting the dry mixture with the antibody, which becomes attached (via lysine residues) to the specially treated surface.
It takes 30 seconds to set up the conjugation, the hands-on time for the conjugation procedure is about 3 minutes and the conjugate is ready to use within 35 minutes. The biomolecule is simply pipetted into a vial containing the latex nanoparticles, then a centrifugation allows a buffer exchange.
The resulting covalent conjugates can be easily resuspended without the need for harsh methods such as sonication or vortexing, unlike traditional conjugation procedures which are prone to aggregation. This is due to the properties of the surface treatment which makes the particles resistant to aggregation.
Additionally, unlike passive methods, the conjugation procedure has only a weak dependence on the isoelectric point of the antibody. Consequently, extensive trials at different pH values are not required; all antibodies can be conjugated at one of two pHs, both of which are supplied in this kit (Reaction Buffers A and B).
Benefits of Latex Conjugation Kit:
Only 30 seconds to set up the conjugation
Three minutes hands-on time for the conjugation procedure
Conjugate is ready to use within 35 minutes – the biomolecule is simply pipetted into a vial containing the latex nanoparticles, then a centrifugation allows a buffer exchange.
This product is manufactured by Expedeon, an Abcam company. It was previously called 400nm Blue Latex Conjugation Kit. Expedeon product code 1000-0040 (4 reaction mini kit) is the same as the 4 x 4 µg size of this product, code 1000-0100 (10 reaction mini kit) is the same as the 10 x 4 µg size, and code 1000-0120 (1 reaction mini kit) is the same as the 1 x 40 µg size of this product.
We would suggest that the antibody to be conjugated should be in 10 – 50 mM MES, HEPES or MOPS at pH 6 – 7 (with no other components e.g. salt or azide) before conjugation. For incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits for Nanoparticles. To learn more about incompatible buffers, please refer to the protocol booklet.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 4 x 4 µg 10 x 4 µg 1 x 40 µg ab273906 - 400 nm Blue Latex vial (Lyophilized) 4 x 4µg 10 x 4µg 1 x 40µg ab273904 - Quencher (10X) 1 x 1500µl 1 x 1500µl 1 x 1500µl ab273902 - Reaction Buffer A (1X) 1 x 1ml 1 x 1ml 1 x 1ml ab273903 - Reaction Buffer B (1X) 1 x 1ml 1 x 1ml 1 x 1ml ab273905 - Resuspension Buffer 1 x 1ml 1 x 1ml 1 x 1ml
The three colours of latex each conjugated to a different antibody or protein, forming a line either by a direct binding event (red and black latex) or a sandwich assay binding event (blue latex). The three colours of latex demonstrate no aggregation or background staining.
Lateral Flow Assay of our specially treated latex beads conjugated to a monoclonal anti-CRP antibody (mAb1) titrated against CRP. 2ng/mL CRP can easily be detected.
Lateral Flow Assay of our specially treated latex conjugated to the monoclonal anti-CRP antibody (mAb2) titrated against CRP in buffer or CRP spiked CRP depleted serum (100 % serum). The latex behaves similarly in serum and buffer, and no aggregation or nonspecific binding is seen.
ab269892 has not yet been referenced specifically in any publications.