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Synthetic peptide within Human LATS1 +LATS2 (N terminal). The exact sequence is proprietary.
Our Abpromise guarantee covers the use of ab70565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/1000 - 1/5000. Detects a band of approximately 150 kDa (predicted molecular weight: 120 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
LATS1 +LATS2 was immunoprecipitated from 1 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate with 6 µg of ab70565. Western blot was performed from the immunoprecipitate using ab70565 at 1 µg/ml.
Lane 1: ab70565 IP in HEK-293T whole cell lysate.
Lane 2: ab70565 IP in HEK-293T whole cell lysate.
Lane 3: control IgG instead of ab70565 in HEK-293T whole cell lysate.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"