Overview

  • Product name

  • Description

    Rabbit polyclonal to LATS1/WARTS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human LATS1/WARTS aa 1-280.
    Database link: O95835

  • Positive control

    • IHC-P: Human liver cancer, placenta and adrenal gland tissues. ICC/IF: HeLa cells. IP: K562 cell lysate.
  • General notes

     This product was previously labelled as LATS1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab234820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.
ICC/IF 1/50 - 1/200.
IP 1/200 - 1/2000.

Target

  • Function

    Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function.
  • Tissue specificity

    Expressed in all adult tissues examined except for lung and kidney.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 UBA domain.
  • Post-translational
    modifications

    Autophosphorylated and phosphorylated during M-phase of the cell cycle. Phosphorylated by STK3/MST2 at Ser-909 and Thr-1079, which results in its activation. Phosphorylation at Ser-464 by NUAK1 and NUAK2 leads to decreased protein level and is required to regulate cellular senescence and cellular ploidy.
  • Cellular localization

    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, during mitosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • h-warts antibody
    • Large tumor suppressor homolog 1 antibody
    • LATS large tumor suppressor homolog 1 antibody
    • LATS1 antibody
    • LATS1_HUMAN antibody
    • Serine threonine protein kinase LATS1 antibody
    • Serine/threonine-protein kinase LATS1 antibody
    • WARTS antibody
    • WARTS protein kinase antibody
    • wts antibody
    see all

Images

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for LATS1/WARTS (green) using ab234820 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).

  • Paraffin-embedded human liver cancer tissue stained for LATS1/WARTS using ab234820 at 1/100 dilution in immunohistochemical analysis.

  • LATS1/WARTS was immunoprecipitated from 500 µg K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with 6 µg ab234820. 

    Lane 1: Rabbit control IgG IP (1 µg) in K562 whole cell lysate.
    Lane 2: ab234820 IP in K562 whole cell lysate.
    Lane 3: K562 whole cell lysate 10 µg (Input).

    For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.

  • Paraffin-embedded human placenta tissue stained for LATS1/WARTS using ab234820 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human liver cancer tissue stained for LATS1/WARTS using ab234820 at 1/100 dilution in immunohistochemical analysis.

    Performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody.

  • Paraffin-embedded human adrenal gland tissue stained for LATS1/WARTS using ab234820 at 1/100 dilution in immunohistochemical analysis.

    Performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody.

References

ab234820 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab234820.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up