Product nameAnti-LATS1/WARTS antibody
See all LATS1/WARTS primary antibodies
DescriptionRabbit polyclonal to LATS1/WARTS
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Horse, Dog, Chimpanzee, Gorilla, Chinese hamster
Synthetic peptide corresponding to Human LATS1/WARTS aa 50-100.
Database link: O95835
- Whole cell lysate from HeLa Cells.
This product was previously labelled as LATS1
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab70561 was affinity purified using an epitope specific to LATS1/WARTS immobilized on solid support.
Our Abpromise guarantee covers the use of ab70561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/15000. Detects a band of approximately 160 kDa (predicted molecular weight: 127 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionNegative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function.
Tissue specificityExpressed in all adult tissues examined except for lung and kidney.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain.
modificationsAutophosphorylated and phosphorylated during M-phase of the cell cycle. Phosphorylated by STK3/MST2 at Ser-909 and Thr-1079, which results in its activation. Phosphorylation at Ser-464 by NUAK1 and NUAK2 leads to decreased protein level and is required to regulate cellular senescence and cellular ploidy.
Cellular localizationCytoplasm, cytoskeleton, microtubule organizing center, centrosome. Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, during mitosis.
- Information by UniProt
- h-warts antibody
- Large tumor suppressor homolog 1 antibody
- LATS large tumor suppressor homolog 1 antibody
All lanes : Anti-LATS1/WARTS antibody (ab70561) at 0.02 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Predicted band size: 127 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Additional bands at: 117 kDa, 200 kDa, 268 kDa, 71 kDa. We are unsure as to the identity of these extra bands.
Detection of human LATS1/WARTS by Immunoprecipitation in whole cell lysate from HeLa cells (1 mg, 1/4 of immunoprecipitate loaded/lane), using ab70561 at 3 µg/mg of lysate (lane 1).
LATS1/WARTS was also immunoprecipitated with an other LATS1/WARTS antibody (lanes 2) and with ab70562 (lane 3) using 3 µg/mg lysate.
Lane 4: Control IgG immunoprecipitate.
Subsequent Western blot detection of LATS1/WARTS was performed using ab70561 at 0.1 µg/ml.
This product has been referenced in:
- Su X et al. ELK1-induced upregulation of long non-coding RNA MIR100HG predicts poor prognosis and promotes the progression of osteosarcoma by epigenetically silencing LATS1 and LATS2. Biomed Pharmacother 109:788-797 (2019). Read more (PubMed: 30551532) »
- Xu Y et al. A miRNA-HERC4 pathway promotes breast tumorigenesis by inactivating tumor suppressor LATS1. Protein Cell 10:595-605 (2019). Read more (PubMed: 30710319) »