• Product name

  • Description

    Rabbit polyclonal to LATS1/WARTS
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human LATS1/WARTS aa 1075-1130 (C terminal).
    Database link: O95835

  • Positive control

    • HeLa whole cell lysate
  • General notes

     This product was previously labelled as LATS1




Our Abpromise guarantee covers the use of ab70562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 127 kDa).
IP Use at 2-5 µg/mg of lysate.


  • Function

    Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function.
  • Tissue specificity

    Expressed in all adult tissues examined except for lung and kidney.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 UBA domain.
  • Post-translational

    Autophosphorylated and phosphorylated during M-phase of the cell cycle. Phosphorylated by STK3/MST2 at Ser-909 and Thr-1079, which results in its activation. Phosphorylation at Ser-464 by NUAK1 and NUAK2 leads to decreased protein level and is required to regulate cellular senescence and cellular ploidy.
  • Cellular localization

    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, during mitosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • h-warts antibody
    • Large tumor suppressor homolog 1 antibody
    • LATS large tumor suppressor homolog 1 antibody
    • LATS1 antibody
    • LATS1_HUMAN antibody
    • Serine threonine protein kinase LATS1 antibody
    • Serine/threonine-protein kinase LATS1 antibody
    • WARTS antibody
    • WARTS protein kinase antibody
    • wts antibody
    see all


  • All lanes : Anti-LATS1/WARTS antibody (ab70562) at 0.4 µg/ml

    Lane 1 : Hela whole cell lysate at 50 µg
    Lane 2 : Hela whole cell lysate at 15 µg

    Developed using the ECL technique.

    Predicted band size: 127 kDa
    Observed band size: 160 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 350 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 10 seconds
  • Detection of human LATS1/WARTS by Immunoprecipitation in whole cell lysate from HeLa cells (1 mg, 1/4 of immunoprecipitate loaded per lane), using ab70562 at 3 µg/mg of lysate (lane 3). LATS1/WARTS was also immunoprecipitated with ab70562 (lane 1) and with an other LATS1/WARTS antibody (lane 2) using 3 µg/mg lysate. Lane 4: Control IgG immunoprecipitate.
    Subsequent Western blot detection of LATS1/WARTS was performed using ab70562 at 0.4 µg/ml.
    Detection: Chemiluminescence with an exposure time of 10 seconds.


This product has been referenced in:

  • Zhang C  et al. AMOT130 linking F-actin to YAP is involved in intervertebral disc degeneration. Cell Prolif N/A:e12492 (2018). Read more (PubMed: 30039887) »
  • Zhang C  et al. Dysregulation of YAP by the Hippo pathway is involved in intervertebral disc degeneration, cell contact inhibition, and cell senescence. Oncotarget 9:2175-2192 (2018). Read more (PubMed: 29416763) »
See all 5 Publications for this product

Customer reviews and Q&As

Western blot
Rat Cell lysate - whole cell (Dissociated hippocampal neurons)
Gel Running Conditions
Reduced Denaturing (8 % SDS-PAGE)
Loading amount
15 µg
Dissociated hippocampal neurons
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Mrs. Crystal Milligan

Verified customer

Submitted Aug 16 2016

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