Overview

  • Product name

  • Description

    Rabbit polyclonal to LATS1/WARTS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human LATS1/WARTS aa 1075-1130 (C terminal).
    Database link: O95835

  • Positive control

    • HeLa whole cell lysate
  • General notes

     This product was previously labelled as LATS1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab70562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 127 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function.
  • Tissue specificity

    Expressed in all adult tissues examined except for lung and kidney.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 UBA domain.
  • Post-translational
    modifications

    Autophosphorylated and phosphorylated during M-phase of the cell cycle. Phosphorylated by STK3/MST2 at Ser-909 and Thr-1079, which results in its activation. Phosphorylation at Ser-464 by NUAK1 and NUAK2 leads to decreased protein level and is required to regulate cellular senescence and cellular ploidy.
  • Cellular localization

    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, during mitosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • h-warts antibody
    • Large tumor suppressor homolog 1 antibody
    • LATS large tumor suppressor homolog 1 antibody
    • LATS1 antibody
    • LATS1_HUMAN antibody
    • Serine threonine protein kinase LATS1 antibody
    • Serine/threonine-protein kinase LATS1 antibody
    • WARTS antibody
    • WARTS protein kinase antibody
    • wts antibody
    see all

Images

  • All lanes : Anti-LATS1/WARTS antibody (ab70562) at 0.4 µg/ml

    Lane 1 : Hela whole cell lysate at 50 µg
    Lane 2 : Hela whole cell lysate at 15 µg

    Developed using the ECL technique.

    Predicted band size: 127 kDa
    Observed band size: 160 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 350 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 10 seconds
  • Detection of human LATS1/WARTS by Immunoprecipitation in whole cell lysate from HeLa cells (1 mg, 1/4 of immunoprecipitate loaded per lane), using ab70562 at 3 µg/mg of lysate (lane 3). LATS1/WARTS was also immunoprecipitated with ab70562 (lane 1) and with an other LATS1/WARTS antibody (lane 2) using 3 µg/mg lysate. Lane 4: Control IgG immunoprecipitate.
    Subsequent Western blot detection of LATS1/WARTS was performed using ab70562 at 0.4 µg/ml.
    Detection: Chemiluminescence with an exposure time of 10 seconds.

References

This product has been referenced in:

  • Zhang C  et al. AMOT130 linking F-actin to YAP is involved in intervertebral disc degeneration. Cell Prolif N/A:e12492 (2018). Read more (PubMed: 30039887) »
  • Zhang C  et al. Dysregulation of YAP by the Hippo pathway is involved in intervertebral disc degeneration, cell contact inhibition, and cell senescence. Oncotarget 9:2175-2192 (2018). Read more (PubMed: 29416763) »
See all 5 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Rat Cell lysate - whole cell (Dissociated hippocampal neurons)
Gel Running Conditions
Reduced Denaturing (8 % SDS-PAGE)
Loading amount
15 µg
Specification
Dissociated hippocampal neurons
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Mrs. Crystal Milligan

Verified customer

Submitted Aug 16 2016

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