Key features and details
- Rabbit polyclonal to LATS1/WARTS
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LATS1/WARTS antibody
See all LATS1/WARTS primary antibodies
DescriptionRabbit polyclonal to LATS1/WARTS
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human LATS1/WARTS aa 1075-1130 (C terminal).
Database link: O95835
- HeLa whole cell lysate
This product was previously labelled as LATS1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab70562 was affinity purified using an epitope specific to LATS1/WARTS immobilized on solid support.
Our Abpromise guarantee covers the use of ab70562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 127 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionNegative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function.
Tissue specificityExpressed in all adult tissues examined except for lung and kidney.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain.
modificationsAutophosphorylated and phosphorylated during M-phase of the cell cycle. Phosphorylated by STK3/MST2 at Ser-909 and Thr-1079, which results in its activation. Phosphorylation at Ser-464 by NUAK1 and NUAK2 leads to decreased protein level and is required to regulate cellular senescence and cellular ploidy.
Cellular localizationCytoplasm, cytoskeleton, microtubule organizing center, centrosome. Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, during mitosis.
- Information by UniProt
- h-warts antibody
- Large tumor suppressor homolog 1 antibody
- LATS large tumor suppressor homolog 1 antibody
All lanes : Anti-LATS1/WARTS antibody (ab70562) at 0.4 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg
Lane 2 : Hela whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 127 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Additional bands at: 350 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 seconds
Detection of human LATS1/WARTS by Immunoprecipitation in whole cell lysate from HeLa cells (1 mg, 1/4 of immunoprecipitate loaded per lane), using ab70562 at 3 µg/mg of lysate (lane 3). LATS1/WARTS was also immunoprecipitated with ab70562 (lane 1) and with an other LATS1/WARTS antibody (lane 2) using 3 µg/mg lysate. Lane 4: Control IgG immunoprecipitate.
Subsequent Western blot detection of LATS1/WARTS was performed using ab70562 at 0.4 µg/ml.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab70562 has been referenced in 7 publications.
- Popow O et al. Identification of Endogenous Adenomatous Polyposis Coli Interaction Partners and ß-Catenin-Independent Targets by Proteomics. Mol Cancer Res 17:1828-1841 (2019). PubMed: 31160382
- Zhang C et al. Dysregulation of YAP by the Hippo pathway is involved in intervertebral disc degeneration, cell contact inhibition, and cell senescence. Oncotarget 9:2175-2192 (2018). PubMed: 29416763
- Lin M et al. Yes-associated protein mediates angiotensin II-induced vascular smooth muscle cell phenotypic modulation and hypertensive vascular remodelling. Cell Prolif 51:e12517 (2018). PubMed: 30156340
- Zhang C et al. AMOT130 linking F-actin to YAP is involved in intervertebral disc degeneration. Cell Prolif N/A:e12492 (2018). PubMed: 30039887
- Sorrentino G et al. Glucocorticoid receptor signalling activates YAP in breast cancer. Nat Commun 8:14073 (2017). PubMed: 28102225
- Vahid S et al. Molecular chaperone Hsp27 regulates the Hippo tumor suppressor pathway in cancer. Sci Rep 6:31842 (2016). PubMed: 27555231
- Liu S et al. miR-21 modulates resistance of HR-HPV positive cervical cancer cells to radiation through targeting LATS1. Biochem Biophys Res Commun 459:679-85 (2015). WB ; Human . PubMed: 25769949