Product nameAnti-LATS1/WARTS antibody
See all LATS1/WARTS primary antibodies
DescriptionRabbit polyclonal to LATS1/WARTS
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human LATS1/WARTS aa 1075-1130 (C terminal).
Database link: O95835
- HeLa whole cell lysate
This product was previously labelled as LATS1
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab70562 was affinity purified using an epitope specific to LATS1/WARTS immobilized on solid support.
Our Abpromise guarantee covers the use of ab70562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 127 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionNegative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function.
Tissue specificityExpressed in all adult tissues examined except for lung and kidney.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain.
modificationsAutophosphorylated and phosphorylated during M-phase of the cell cycle. Phosphorylated by STK3/MST2 at Ser-909 and Thr-1079, which results in its activation. Phosphorylation at Ser-464 by NUAK1 and NUAK2 leads to decreased protein level and is required to regulate cellular senescence and cellular ploidy.
Cellular localizationCytoplasm, cytoskeleton, microtubule organizing center, centrosome. Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, during mitosis.
- Information by UniProt
- h-warts antibody
- Large tumor suppressor homolog 1 antibody
- LATS large tumor suppressor homolog 1 antibody
All lanes : Anti-LATS1/WARTS antibody (ab70562) at 0.4 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg
Lane 2 : Hela whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 127 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Additional bands at: 350 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 seconds
Detection of human LATS1/WARTS by Immunoprecipitation in whole cell lysate from HeLa cells (1 mg, 1/4 of immunoprecipitate loaded per lane), using ab70562 at 3 µg/mg of lysate (lane 3). LATS1/WARTS was also immunoprecipitated with ab70562 (lane 1) and with an other LATS1/WARTS antibody (lane 2) using 3 µg/mg lysate. Lane 4: Control IgG immunoprecipitate.
Subsequent Western blot detection of LATS1/WARTS was performed using ab70562 at 0.4 µg/ml.
Detection: Chemiluminescence with an exposure time of 10 seconds.
This product has been referenced in:
- Zhang C et al. AMOT130 linking F-actin to YAP is involved in intervertebral disc degeneration. Cell Prolif N/A:e12492 (2018). Read more (PubMed: 30039887) »
- Zhang C et al. Dysregulation of YAP by the Hippo pathway is involved in intervertebral disc degeneration, cell contact inhibition, and cell senescence. Oncotarget 9:2175-2192 (2018). Read more (PubMed: 29416763) »