Overview

  • Product name
    Anti-LATS2 antibody - C-terminal
    See all LATS2 primary antibodies
  • Description
    Rabbit polyclonal to LATS2 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide within Mouse LATS2 aa 999-1027 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q7TSJ6

  • Positive control
    • Mouse testis tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab174499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 115 kDa.

Target

  • Function
    Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor.
  • Tissue specificity
    Expressed at high levels in heart and skeletal muscle and at lower levels in all other tissues examined.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 UBA domain.
  • Post-translational
    modifications
    Autophosphorylated and phosphorylated during M-phase and the G1/S-phase of the cell cycle. Phosphorylated and activated by STK3.
  • Cellular localization
    Cytoplasm > cytoskeleton > centrosome. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Co-localizes with STK6 at the centrosomes during interphase, early prophase and cytokinesis. Migrates to the spindle poles during mitosis, and to the midbody during cytokinesis.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ13161 antibody
    • Kinase phosphorylated during mitosis protein antibody
    • KPM antibody
    • Large tumor suppressor homolog 2 antibody
    • Large tumor suppressor homolog 2 Drosophila antibody
    • Large tumor suppressor kinase 2 antibody
    • LATS large tumor suppressor Drosophila homolog 2 antibody
    • LATS large tumor suppressor homolog 2 antibody
    • Lats2 antibody
    • LATS2_HUMAN antibody
    • Serine/threonine kinase kpm antibody
    • Serine/threonine protein kinase kpm antibody
    • Serine/threonine-protein kinase kpm antibody
    • Serine/threonine-protein kinase LATS2 antibody
    • Warts like kinase antibody
    • Warts-like kinase antibody
    see all

Images

  • Anti-LATS2 antibody - C-terminal (ab174499) at 1/100 dilution + Mouse testis tissue lysate at 35 µg

    Predicted band size: 115 kDa

References

This product has been referenced in:
  • Ye Y  et al. microRNA-605 promotes cell proliferation, migration and invasion in non-small cell lung cancer by directly targeting LATS2. Exp Ther Med 14:867-873 (2017). Read more (PubMed: 28673012) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Dog Tissue lysate - whole (mammary gland)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
mammary gland
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 25 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate-based buffer
Permeabilization
No
Specification
testis
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
formalin
Username

Abcam user community

Verified customer

Submitted Oct 25 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (mammary gland)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate-based buffer
Permeabilization
No
Specification
mammary gland
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formalin
Username

Abcam user community

Verified customer

Submitted Oct 25 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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