Overview

  • Product name

  • Description

    Rabbit polyclonal to LAYN
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human LAYN (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q6UX15

  • Positive control

    • Whole extract of HEK-293T cells overexpressing Human LAYN; A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab214015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 43 kDa.
ICC/IF Use a concentration of 2.5 - 5 µg/ml.

Target

  • Function

    Receptor for hyaluronate.
  • Sequence similarities

    Contains 1 C-type lectin domain.
  • Domain

    The C-terminal domain interacts with the N-terminal domain of RDX.
  • Cellular localization

    Membrane. Colocalizes with TLN1 at the membrane ruffles.
  • Information by UniProt
  • Database links

  • Alternative names

    • Layilin antibody
    • LAYN antibody
    • LAYN_HUMAN antibody
    • UNQ208/PRO234 antibody

Images

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.5% Triton X-100 permeabilized A549 cells labeling LAYN with ab214015 at 2.5 μg/ml, followed by Goat Anti-Rabbit IgG, Atto488 conjugate.

  • Lane 1 : Anti-LAYN antibody (ab214015) at 1 µg/ml
    Lanes 2-3 : Anti-LAYN antibody (ab214015) at 2 µg/ml

    Lanes 1-2 : Whole extract of HEK-293T cells overexpressing Human LAYN
    Lane 3 : Whole extract of HEK-293T cells overexpressing Human LAYN with LAYN immunizing peptide

    Secondary
    All lanes : Goat Anti-Rabbit IgG-Peroxidase

    Developed using the ECL technique.

    Predicted band size: 43 kDa

References

ab214015 has not yet been referenced specifically in any publications.

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