Recombinant Anti-LBP antibody [EPR10865] - BSA and Azide free (ab249522)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10865] to LBP - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-LBP antibody [EPR10865] - BSA and Azide free
See all LBP primary antibodies -
Description
Rabbit monoclonal [EPR10865] to LBP - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab249522 is the carrier-free version of ab169776.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR10865 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).
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Notes |
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IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa). |
Target
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Function
Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. -
Sequence similarities
Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3929 Human
- Omim: 151990 Human
- SwissProt: P18428 Human
- Unigene: 154078 Human
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Alternative names
- BPI fold containing family D, member 2 antibody
- Bpifd2 antibody
- LBP antibody
see all
Images
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This data was developed using ab169776, the same antibody clone in a different buffer formulation.
All lanes : Anti-LBP antibody [EPR10865] (ab169776) at 1/1000 dilution
Lane 1 : Immunoprecipitation of LBP from Human plasma lysate using ab169776 at 1/10 dilution
Lane 2 : 1 X PBS Negative control
Secondary
All lanes : HRP-conjugated anti-rabbit IgG -
All lanes : Anti-LBP antibody [EPR10865] (ab169776) at 1/1000 dilution
Lane 1 : Human serum lysate
Lane 2 : Human plasma lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 53 kDaThis data was developed using ab169776, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab249522 has not yet been referenced specifically in any publications.