Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal to LC3A - BSA and Azide free (Capture)
- Suitable for: Sandwich ELISA
- Reacts with: Human
Product nameAnti-LC3A antibody - BSA and Azide free (Capture)
See all LC3A primary antibodies
DescriptionRabbit monoclonal to LC3A - BSA and Azide free (Capture)
Tested applicationsSuitable for: Sandwich ELISAmore details
Species reactivityReacts with: Human
ab244816 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human LC3A. The recommended pair for sandwich ELISA is:
Capture: ab244816, Human LC3A Capture Antibody (unconjugated)
Detector: ab245089, Human LC3A Detector Antibody (unconjugated)
The reference range value is 39.06 - 2500 pg/ml.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
-High batch-to-batch consistency and reproducibility
-Improved sensitivity and specificity
-Long-term security of supply
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferConstituent: 100% PBS
Concentration information loading...
sELISA pair antibody
Our Abpromise guarantee covers the use of ab244816 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal to LC3A - BSA and Azide free (Detector) (ab245089).|
RelevanceA major contributor to cellular homeostasis is the ability of the cell to strike a balance between the formation and degradation/removal of its cellular components. This process of internal cellular turn-over is called autophagy (self-eating), and is facilitated by a pathway of around 16 interacting proteins in the human. LC3, a ubiquitin-like modifier protein, is the human homolog of yeast Apg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. LC3 is expressed as 3 splice variants (LC3A, LC3B and LC3C), which exhibit different tissue distributions and are processed into cytosolic and autophagosomal membrane-bound forms, termed LC3-I and LC3-II, respectively. A disruption to the autophagic process is now associated with the progression of several cancers, neurodegenerative disorders and cardiac pathologies, where LC3 is widely employed as a marker for autophagy.
Cellular localizationLC3-I is located in the Cytoplasm. LC3-II is found on intracytoplasmic membranes and the autophagosomal membrane.
- ATG 8 antibody
- Autophagy related ubiquitin-like modifier ATG8 antibody
- Autophagy-related protein 8 antibody
ab244816 has not yet been referenced specifically in any publications.