Anti-LC3A/B antibody (ab128025)
Key features and details
- Rabbit polyclonal to LC3A/B
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-LC3A/B antibody
See all LC3A/B primary antibodies -
Description
Rabbit polyclonal to LC3A/B -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide corresponding to Human LC3A/B (C terminal).
Database link: Q9GZQ8 -
Positive control
- HeLa whole cell lysates
Properties
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Form
Liquid -
Storage instructions
Frozen Stock (-20C). Shelf life 12 months. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 99% Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Mouse
Human
|
| All applications |
Rat
Cow
Drosophila melanogaster
Non human primates
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
Use a concentration of 2 µg/ml. Predicted molecular weight: 15 kDa.
|
| Notes |
|---|
|
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 15 kDa. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 81631 Human
- Entrez Gene: 84557 Human
- Entrez Gene: 66734 Mouse
- Entrez Gene: 67443 Mouse
- Entrez Gene: 362245 Rat
- Entrez Gene: 64862 Rat
- Omim: 601242 Human
- Omim: 609604 Human
see all -
Alternative names
- ATG8F antibody
- Autophagy related protein LC3 A antibody
- Autophagy related protein LC3 B antibody
see all
Images
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Western blot - Anti-LC3A/B antibody (ab128025)All lanes : Anti-LC3A/B antibody (ab128025) at 1/1000 dilution
Lane 1 : HeLa Whole Cell Lysate
Lane 2 : HeLa + Chloroquine Whole Cell Lysate
Lane 3 : NIH3T3 Whole Cell Lysate
Lane 4 : U87-MG Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 14,16 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThis blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab128025 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
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Western blot - Anti-LC3A/B antibody (ab128025)All lanes : Anti-LC3A/B antibody (ab128025) at 2 µg/ml
Lane 1 : WT mouse ES cell lysate
Lane 2 : atg5-/-
Predicted band size: 15 kDa
Protocols
References (67)
ab128025 has been referenced in 67 publications.
- Li R et al. Nerve growth factor activates autophagy in Schwann cells to enhance myelin debris clearance and to expedite nerve regeneration. Theranostics 10:1649-1677 (2020). PubMed: 32042328
- Cui C et al. Relationship between autophagy, apoptosis and endoplasmic reticulum stress induced by melatonin in osteoblasts by septin7 expression. Mol Med Rep 21:2427-2434 (2020). PubMed: 32323792
- Zhang Z et al. Driving GABAergic neurons optogenetically improves learning, reduces amyloid load and enhances autophagy in a mouse model of Alzheimer's disease. Biochem Biophys Res Commun 525:928-935 (2020). PubMed: 32173530
- Hu L et al. The Influence of Bcl-3 Expression on Cell Migration and Chemosensitivity of Gastric Cancer Cells via Regulating Hypoxia-Induced Protective Autophagy. J Gastric Cancer 20:95-105 (2020). PubMed: 32269848
- Zhang S et al. HMGB1/RAGE axis mediates stress-induced RVLM neuroinflammation in mice via impairing mitophagy flux in microglia. J Neuroinflammation 17:15 (2020). PubMed: 31924219
Customer reviews and Q&As
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