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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microtubules MT Associated Proteins MAP
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Anti-LC3A/B antibody (ab128025)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (67)

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Western blot - Anti-LC3A/B antibody (ab128025)
  • Western blot - Anti-LC3A/B antibody (ab128025)

Key features and details

  • Rabbit polyclonal to LC3A/B
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-LC3B antibody [EPR18709] - Autophagosome Marker (ab192890)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-LC3A/B antibody
    See all LC3A/B primary antibodies
  • Description

    Rabbit polyclonal to LC3A/B
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human LC3A/B (C terminal).
    Database link: Q9GZQ8

  • Positive control

    • HeLa whole cell lysates

Properties

  • Form

    Liquid
  • Storage instructions

    Frozen Stock (-20C). Shelf life 12 months.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: 99% Tris citrate/phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • MT Associated Proteins
    • MAP
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Cardiovascular
    • Heart
    • Autophagy
    • Autophagosome
    • Cardiovascular
    • Heart
    • Autophagy
    • APG gene products
    • Cancer
    • Signal transduction
    • Autophagy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • APG gene products
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • Autophagosome
    • Cancer
    • Cell Death
    • Autophagy
    • Signal Transduction
    • Cancer
    • Cell Death
    • Autophagy
    • APG gene products
    • Cancer
    • Cell Death
    • Autophagy
    • Autophagosome

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab128025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Mouse
Human
All applications
Rat
Cow
Drosophila melanogaster
Non human primates
Application Abreviews Notes
WB (2)
Use a concentration of 2 µg/ml. Predicted molecular weight: 15 kDa.
Notes
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 15 kDa.

Target

  • Target information above from: UniProt accession Q9GZQ8 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 81631 Human
    • Entrez Gene: 84557 Human
    • Entrez Gene: 66734 Mouse
    • Entrez Gene: 67443 Mouse
    • Entrez Gene: 362245 Rat
    • Entrez Gene: 64862 Rat
    • Omim: 601242 Human
    • Omim: 609604 Human
    • SwissProt: Q9GZQ8 Human
    • SwissProt: Q9H492 Human
    • SwissProt: Q91VR7 Mouse
    • SwissProt: Q9CQV6 Mouse
    • SwissProt: Q62625 Rat
    • SwissProt: Q6XVN8 Rat
    • Unigene: 356061 Human
    • Unigene: 196239 Mouse
    • Unigene: 28357 Mouse
    • Unigene: 41412 Rat
    see all
  • Alternative names

    • ATG8F antibody
    • Autophagy related protein LC3 A antibody
    • Autophagy related protein LC3 B antibody
    • Autophagy related ubiquitin like modifier LC3 A antibody
    • Autophagy related ubiquitin like modifier LC3 B antibody
    • Autophagy-related protein LC3 B antibody
    • Autophagy-related ubiquitin-like modifier LC3 B antibody
    • LC3 antibody
    • MAP1 light chain 3 like protein 1 antibody
    • MAP1 light chain 3 like protein 2 antibody
    • MAP1 light chain 3-like protein 2 antibody
    • MAP1A/1B light chain 3 A antibody
    • MAP1A/1B light chain 3 B antibody
    • MAP1A/1BLC3 antibody
    • MAP1A/MAP1B LC3 A antibody
    • MAP1A/MAP1B LC3 B antibody
    • MAP1A/MAP1B light chain 3 B antibody
    • MAP1ALC3 antibody
    • MAP1BLC3 antibody
    • MAP1LC3A antibody
    • Map1lc3b antibody
    • Microtubule associated protein 1 light chain 3 alpha antibody
    • Microtubule associated protein 1 light chain 3 beta antibody
    • Microtubule associated proteins 1A/1B light chain 3 antibody
    • Microtubule associated proteins 1A/1B light chain 3A antibody
    • Microtubule associated proteins 1A/1B light chain 3B antibody
    • Microtubule-associated protein 1 light chain 3 beta antibody
    • Microtubule-associated proteins 1A/1B light chain 3B antibody
    • MLP3B_HUMAN antibody
    see all

Images

  • Western blot - Anti-LC3A/B antibody (ab128025)
    Western blot - Anti-LC3A/B antibody (ab128025)
    All lanes : Anti-LC3A/B antibody (ab128025) at 1/1000 dilution

    Lane 1 : HeLa Whole Cell Lysate
    Lane 2 : HeLa + Chloroquine Whole Cell Lysate
    Lane 3 : NIH3T3 Whole Cell Lysate
    Lane 4 : U87-MG Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Observed band size: 14,16 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab128025 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • Western blot - Anti-LC3A/B antibody (ab128025)
    Western blot - Anti-LC3A/B antibody (ab128025)
    All lanes : Anti-LC3A/B antibody (ab128025) at 2 µg/ml

    Lane 1 : WT mouse ES cell lysate
    Lane 2 : atg5-/-

    Predicted band size: 15 kDa

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (67)

    Publishing research using ab128025? Please let us know so that we can cite the reference in this datasheet.

    ab128025 has been referenced in 67 publications.

    • Li R  et al. Nerve growth factor activates autophagy in Schwann cells to enhance myelin debris clearance and to expedite nerve regeneration. Theranostics 10:1649-1677 (2020). PubMed: 32042328
    • Cui C  et al. Relationship between autophagy, apoptosis and endoplasmic reticulum stress induced by melatonin in osteoblasts by septin7 expression. Mol Med Rep 21:2427-2434 (2020). PubMed: 32323792
    • Zhang Z  et al. Driving GABAergic neurons optogenetically improves learning, reduces amyloid load and enhances autophagy in a mouse model of Alzheimer's disease. Biochem Biophys Res Commun 525:928-935 (2020). PubMed: 32173530
    • Hu L  et al. The Influence of Bcl-3 Expression on Cell Migration and Chemosensitivity of Gastric Cancer Cells via Regulating Hypoxia-Induced Protective Autophagy. J Gastric Cancer 20:95-105 (2020). PubMed: 32269848
    • Zhang S  et al. HMGB1/RAGE axis mediates stress-induced RVLM neuroinflammation in mice via impairing mitophagy flux in microglia. J Neuroinflammation 17:15 (2020). PubMed: 31924219
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-LC3A/B antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (15% Gel)
    Sample
    Mouse Tissue lysate - whole (Skeletal muscle)
    Specification
    Skeletal muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 12 2014

    Western blot abreview for Anti-LC3A/B antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (15% Gel)
    Sample
    Rat Tissue lysate - whole (Skeletal muscle)
    Specification
    Skeletal muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 05 2014

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