Product nameAnti-LC3A/B antibody
See all LC3A/B primary antibodies
DescriptionRabbit polyclonal to LC3A/B
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Cow, Human, Drosophila melanogaster, Non human primates
Synthetic peptide corresponding to Human LC3A/B (C terminal).
Database link: Q9GZQ8
- HeLa whole cell lysates Human Cerebral Cortex Mouse ES cell lysate
Storage instructionsFrozen Stock (-20C). Shelf life 12 months.
Storage bufferPreservative: 0.1% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab128025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Predicted molecular weight: 15 kDa.|
|IHC-P||Use a concentration of 5 - 10 µg/ml.|
- Information by UniProt
- ATG8F antibody
- Autophagy related protein LC3 A antibody
- Autophagy related protein LC3 B antibody
All lanes : Anti-LC3A/B antibody (ab128025) at 1/1000 dilution
Lane 1 : HeLa Whole Cell Lysate
Lane 2 : HeLa + Chloroquine Whole Cell Lysate
Lane 3 : NIH3T3 Whole Cell Lysate
Lane 4 : U87-MG Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 14,16 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab128025 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
All lanes : Anti-LC3A/B antibody (ab128025) at 2 µg/ml
Lane 1 : WT
Lane 2 : atg5-/-
Predicted band size: 15 kDa
ab128025 staining LC3A/B in MEF cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 1% Triton X-100 and blocked with 10% goat serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100 in 10% goat serum) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.
This product has been referenced in:
- Chen H et al. MicroRNA-155 affects oxidative damage through regulating autophagy in endothelial cells. Oncol Lett 17:2237-2243 (2019). Read more (PubMed: 30675289) »
- Zhang E et al. Minocycline promotes cardiomyocyte mitochondrial autophagy and cardiomyocyte autophagy to prevent sepsis-induced cardiac dysfunction by Akt/mTOR signaling. Apoptosis 24:369-381 (2019). Read more (PubMed: 30756206) »