Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab62721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16 - 18 kDa).|
|IP||Use at an assay dependent dilution.|
- Information by UniProt
- ATG8F antibody
- Autophagy related protein LC3 A antibody
- Autophagy related protein LC3 B antibody
Lane 1 : Anti-LC3A/B antibody (ab62721) at 0.5 µg/ml
Lanes 2-3 : Anti-LC3A/B antibody (ab62721) at 1 µg/ml
Lanes 1-2 : Rat whole brain extract
Lane 3 : Rat whole brain extract with immunizing peptide
All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate
Developed using the ECL technique.
Predicted band size: 16 - 18 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Wu J et al. Long noncoding RNA UCA1 targets miR-582-5p and contributes to the progression and drug resistance of bladder cancer cells through ATG7-mediated autophagy inhibition. Onco Targets Ther 12:495-508 (2019). Read more (PubMed: 30666128) »
- Dong H et al. Poloxamer 188 rescues MPTP-induced lysosomal membrane integrity impairment in cellular and mouse models of Parkinson's disease. Neurochem Int 126:178-186 (2019). Read more (PubMed: 30904670) »