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Rabbit polyclonal to LC3A/B
Mouse, Rat, Human
Synthetic peptide corresponding to Human LC3A/B aa 36-49 conjugated to Keyhole Limpet Haemocyanin (KLH) (Cysteine residue). Also SwissProt ID: Q9GZQ8
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16 - 18 kDa).
Use at an assay dependent dilution.
Information by UniProt
Autophagy related protein LC3 A antibody
Autophagy related protein LC3 B antibody
Western blot - Anti-LC3A/B antibody (ab62721)
Lane 1 :
Anti-LC3A/B antibody (ab62721) at 0.5 µg/ml
Lanes 2-3 :
Anti-LC3A/B antibody (ab62721) at 1 µg/ml
Lanes 1-2 :
Rat whole brain extract
Lane 3 :
Rat whole brain extract with immunizing peptide
Secondary All lanes :
Goat Anti-Rabbit IgG, Peroxidase conjugate
Developed using the ECL technique.
Predicted band size:
16 - 18 kDa
Observed band size:
18 kDa (
why is the actual band size different from the predicted?
This product has been referenced in:
et al. The Sineoculis Homeobox Homolog 1 (SIX1) Gene Regulates Paclitaxel Resistance by Affecting Reactive Oxygen Species and Autophagy in Human Hepatocellular Carcinoma Cell Line HepG2. Med Sci Monit 24:2271-2279 (2018).
Read more (PubMed: 29656300) »
et al. Long Non-coding RNA H19 Induces Cerebral Ischemia Reperfusion Injury via Activation of Autophagy. Aging Dis 8:71-84 (2017).
Read more (PubMed: 28203482) »
See all 21 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"