Overview

  • Product name

  • Description

    Rabbit polyclonal to LC3A/B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human LC3A/B aa 36-49 conjugated to Keyhole Limpet Haemocyanin (KLH) (Cysteine residue). Also SwissProt ID: Q9GZQ8
    Sequence:

    ERYKGEKQLPVLDK


    Database link: Q9H492

  • Positive control

    • Whole brain. U87 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab62721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16 - 18 kDa).
IP Use at an assay dependent dilution.

Target

  • Information by UniProt
  • Database links

  • Alternative names

    • ATG8F antibody
    • Autophagy related protein LC3 A antibody
    • Autophagy related protein LC3 B antibody
    • Autophagy related ubiquitin like modifier LC3 A antibody
    • Autophagy related ubiquitin like modifier LC3 B antibody
    • Autophagy-related protein LC3 B antibody
    • Autophagy-related ubiquitin-like modifier LC3 B antibody
    • LC3 antibody
    • MAP1 light chain 3 like protein 1 antibody
    • MAP1 light chain 3 like protein 2 antibody
    • MAP1 light chain 3-like protein 2 antibody
    • MAP1A/1B light chain 3 A antibody
    • MAP1A/1B light chain 3 B antibody
    • MAP1A/1BLC3 antibody
    • MAP1A/MAP1B LC3 A antibody
    • MAP1A/MAP1B LC3 B antibody
    • MAP1A/MAP1B light chain 3 B antibody
    • MAP1ALC3 antibody
    • MAP1BLC3 antibody
    • MAP1LC3A antibody
    • Map1lc3b antibody
    • Microtubule associated protein 1 light chain 3 alpha antibody
    • Microtubule associated protein 1 light chain 3 beta antibody
    • Microtubule associated proteins 1A/1B light chain 3 antibody
    • Microtubule associated proteins 1A/1B light chain 3A antibody
    • Microtubule associated proteins 1A/1B light chain 3B antibody
    • Microtubule-associated protein 1 light chain 3 beta antibody
    • Microtubule-associated proteins 1A/1B light chain 3B antibody
    • MLP3B_HUMAN antibody
    see all

Images

  • Lane 1 : Anti-LC3A/B antibody (ab62721) at 0.5 µg/ml
    Lanes 2-3 : Anti-LC3A/B antibody (ab62721) at 1 µg/ml

    Lanes 1-2 : Rat whole brain extract
    Lane 3 : Rat whole brain extract with immunizing peptide

    Secondary
    All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate

    Developed using the ECL technique.

    Predicted band size: 16 - 18 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Wu J  et al. Long noncoding RNA UCA1 targets miR-582-5p and contributes to the progression and drug resistance of bladder cancer cells through ATG7-mediated autophagy inhibition. Onco Targets Ther 12:495-508 (2019). Read more (PubMed: 30666128) »
  • Dong H  et al. Poloxamer 188 rescues MPTP-induced lysosomal membrane integrity impairment in cellular and mouse models of Parkinson's disease. Neurochem Int 126:178-186 (2019). Read more (PubMed: 30904670) »
See all 27 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abreviews
Application
Western blot
Sample
Rabbit Tissue lysate - whole (lung)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
35 µg
Specification
lung
Blocking step
BSA as blocking agent for 1 hour(s) and 45 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted May 19 2017

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Human Cell lysate - whole cell (COV434 cell line)
Specification
COV434 cell line
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C

Abcam user community

Verified customer

Submitted Feb 13 2015

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Mouse Tissue lysate - whole (Muscle)
Specification
Muscle
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C

Abcam user community

Verified customer

Submitted Jan 23 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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