Immunocytochemistry/ Immunofluorescence abreview for Anti-LC3B antibody

Average
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Permeabilization
No

Other product details

Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Rabbit AlexaFluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200

Additional data

Additional Notes
Asynchronous cells were treated with bleomycin, methanol fixed, immunofluorescently labeled and counterstained with DAPI. The antibody produced the expected staining pattern under these conditions. Paraformaldehyde-fixed samples were also evaluated and produced a nuclear enriched staining pattern, which is not what was expected.

Dr. Kirk Mcmanus

Verified customer

Submitted May 10 2013

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