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  1. Link

    lc3b-antibody-epr21234-ab232940.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microtubules MT Associated Proteins MAP
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Validated using a knockout cell lineRecombinant

Recombinant Anti-LC3B antibody [EPR21234] (ab232940)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (3)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LC3B antibody [EPR21234] (ab232940)
  • Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody [EPR21234] (ab232940)
  • Anti-LC3B antibody [EPR21234] (ab232940)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21234] to LC3B
  • Suitable for: ICC/IF, IHC-P
  • Knockout validated
  • Reacts with: Human

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Recombinant Human LC3B protein (ab103506)
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Anti-APG5L/ATG5 antibody [EPR1755(2)] (ab108327)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-LC3B antibody [EPR21234]
    See all LC3B primary antibodies
  • Description

    Rabbit monoclonal [EPR21234] to LC3B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human LC3B.
    Database link: Q9GZQ8

  • Positive control

    • ICC/IF: HAP1 cells (+/- chloroquine), (HAP1-MAP1LC3B knockout cells used as negative cell line). IHC-P: FFPE human normal colon and human normal cerebral cortex tissue sections.
  • General notes

    This product was made using synthetic libraries and phage display technology.

    This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21234
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • MT Associated Proteins
    • MAP
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Cardiovascular
    • Heart
    • Autophagy
    • APG gene products
    • Cancer
    • Signal transduction
    • Autophagy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • APG gene products
    • Cancer
    • Cell Death
    • Autophagy
    • Signal Transduction
    • Cancer
    • Cell Death
    • Autophagy
    • APG gene products
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human LC3B protein (ab103506)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab232940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (1)
Use at an assay dependent concentration.
IHC-P (1)
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Probably involved in formation of autophagosomal vacuoles (autophagosomes).
  • Tissue specificity

    Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.
  • Sequence similarities

    Belongs to the MAP1 LC3 family.
  • Post-translational
    modifications

    The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II.
  • Cellular localization

    Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
  • Target information above from: UniProt accession Q9GZQ8 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 81631 Human
    • Omim: 609604 Human
    • SwissProt: Q9GZQ8 Human
    • Unigene: 356061 Human
    • Alternative names

      • ATG8F antibody
      • Autophagy-related protein LC3 B antibody
      • Autophagy-related ubiquitin-like modifier LC3 B antibody
      • LC3B antibody
      • LC3II antibody
      • MAP1 light chain 3 like protein 2 antibody
      • MAP1 light chain 3-like protein 2 antibody
      • MAP1A/1BLC3 antibody
      • MAP1A/MAP1B LC3 B antibody
      • MAP1A/MAP1B light chain 3 B antibody
      • MAP1ALC3 antibody
      • MAP1LC3B a antibody
      • Map1lc3b antibody
      • Microtubule associated protein 1 light chain 3 beta antibody
      • Microtubule-associated protein 1 light chain 3 beta antibody
      • Microtubule-associated proteins 1A/1B light chain 3B antibody
      • MLP3B_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LC3B antibody [EPR21234] (ab232940)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LC3B antibody [EPR21234] (ab232940)

      IHC image of LC3B staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab232940, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

       

    • Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody [EPR21234] (ab232940)
      Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody [EPR21234] (ab232940)

      ab232940 staining LC3B in HAP1 cells (wildtype and MAP1LC3B knockout) +/- Chloroquine (50μM, 24 hours). The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab232940 at 0.1μg/ml and ab195889 then detected with an Alexa Fluor® 488 goat anti-rabbit secondary antibody (ab150081) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red).

    • Anti-LC3B antibody [EPR21234] (ab232940)
      Anti-LC3B antibody [EPR21234] (ab232940)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab232940? Please let us know so that we can cite the reference in this datasheet.

    ab232940 has been referenced in 3 publications.

    • Zhou Y  et al. lncRNA NEAT1 regulates gastric carcinoma cell proliferation, invasion and apoptosis via the miR-500a-3p/XBP-1 axis. Mol Med Rep 24:N/A (2021). PubMed: 33982777
    • Jiang F  et al. Fluorofenidone attenuates paraquat-induced pulmonary fibrosis by regulating the PI3K/Akt/mTOR signaling pathway and autophagy. Mol Med Rep 23:N/A (2021). PubMed: 33786626
    • Yu Q  et al. miR-155-5p increases the sensitivity of liver cancer cells to adriamycin by regulating ATG5-mediated autophagy. Neoplasma 68:87-95 (2021). PubMed: 32853020

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-LC3B antibody [EPR21234]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Liver
    Blocking step
    sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. David Ivancic

    Verified customer

    Submitted Jun 26 2019

    Western blot abreview for Anti-LC3B antibody [EPR21234]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Skin-derived fibroblasts)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    5 µg
    Treatment
    Autophagy inducer, autophagy blocker, virus
    Specification
    Skin-derived fibroblasts
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 18 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-LC3B antibody [EPR21234]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Skin-derived fibroblasts)
    Permeabilization
    Yes - Triton X100 0.1%
    Specification
    Skin-derived fibroblasts
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 18 2019

    Western blot abreview for Anti-LC3B antibody [EPR21234]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Rat primary cortical neuronal cultures)
    Gel Running Conditions
    Reduced Denaturing (15%)
    Loading amount
    25 µg
    Treatment
    treated or not treated with Bafilomycin A1 (BAF) 300nM for 6h
    Specification
    Rat primary cortical neuronal cultures
    Blocking step
    casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 08 2019

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