Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM293] to LC3B
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Product nameAnti-LC3B antibody [RM293]
See all LC3B primary antibodies
DescriptionRabbit monoclonal [RM293] to LC3B
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human LC3B (N terminal).
Database link: Q9GZQ8
- WB: HeLa cell lysate, untreated or treated with chloroquine. IHC-P: Human brain tissue. ICC/IF: HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityProtein A purified
Purification notesab222776 was purified from an animal origin–free culture supernatant.
Our Abpromise guarantee covers the use of ab222776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/200. Predicted molecular weight: 15 kDa.|
|IHC-P||1/100 - 1/200.|
|ICC/IF||1/100 - 1/200.|
FunctionProbably involved in formation of autophagosomal vacuoles (autophagosomes).
Tissue specificityMost abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.
Sequence similaritiesBelongs to the MAP1 LC3 family.
modificationsThe precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II.
Cellular localizationCytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
- Information by UniProt
- ATG8F antibody
- Autophagy-related protein LC3 B antibody
- Autophagy-related ubiquitin-like modifier LC3 B antibody
All lanes : Anti-LC3B antibody [RM293] (ab222776) at 1/200 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with chloroquine, cell lysate
Predicted band size: 15 kDa
Formalin-fixed, paraffin-embedded human brain tissue stained for LC3B with ab222776 at 1/200 dilution in immunohistochemical analysis.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells, untreated (left panel) or treated with chloroquine (right panel) stained for LC3B (red) using ab222776 at 1/200 dilution in ICC/IF.
Actin filaments were labeled with fluorescein phalloidin (green). Nuclei were labeled with DAPI (blue).
ab222776 has not yet been referenced specifically in any publications.