Recombinant

Recombinant Anti-LC3B antibody [RM293] (ab222776)

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Overview

  • Product name

    Anti-LC3B antibody [RM293]
    See all LC3B primary antibodies

  • Description

    Rabbit monoclonal [RM293] to LC3B

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide corresponding to Human LC3B (N terminal).
    Database link: Q9GZQ8

  • Positive control

    • WB: HeLa cell lysate, untreated or treated with chloroquine. IHC-P: Human brain tissue. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab222776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/200. Predicted molecular weight: 15 kDa.
IHC-P 1/100 - 1/200.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Probably involved in formation of autophagosomal vacuoles (autophagosomes).

  • Tissue specificity

    Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.

  • Sequence similarities

    Belongs to the MAP1 LC3 family.

  • Post-translational
    modifications

    The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II.

  • Cellular localization

    Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ATG8F antibody
    • Autophagy-related protein LC3 B antibody
    • Autophagy-related ubiquitin-like modifier LC3 B antibody
    • LC3B antibody
    • LC3II antibody
    • MAP1 light chain 3 like protein 2 antibody
    • MAP1 light chain 3-like protein 2 antibody
    • MAP1A/1BLC3 antibody
    • MAP1A/MAP1B LC3 B antibody
    • MAP1A/MAP1B light chain 3 B antibody
    • MAP1ALC3 antibody
    • MAP1LC3B a antibody
    • Map1lc3b antibody
    • Microtubule associated protein 1 light chain 3 beta antibody
    • Microtubule-associated protein 1 light chain 3 beta antibody
    • Microtubule-associated proteins 1A/1B light chain 3B antibody
    • MLP3B_HUMAN antibody
    see all

Images

  • Western blot - Anti-LC3B antibody [RM293] (ab222776)
    Western blot - Anti-LC3B antibody [RM293] (ab222776)
    All lanes : Anti-LC3B antibody [RM293] (ab222776) at 1/200 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with chloroquine, cell lysate

    Predicted band size: 15 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LC3B antibody [RM293] (ab222776)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LC3B antibody [RM293] (ab222776)

    Formalin-fixed, paraffin-embedded human brain tissue stained for LC3B with ab222776 at 1/200 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody [RM293] (ab222776)
    Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody [RM293] (ab222776)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells, untreated (left panel) or treated with chloroquine (right panel) stained for LC3B (red) using ab222776 at 1/200 dilution in ICC/IF.

    Actin filaments were labeled with fluorescein phalloidin (green). Nuclei were labeled with DAPI (blue).

References

ab222776 has not yet been referenced specifically in any publications.

Publishing research using ab222776? Please let us know so that we can cite the reference in this datasheet.

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