Recombinant Anti-LC3B antibody [RM293] (ab222776)
![Western blot - Anti-LC3B antibody [RM293] (ab222776)](https://www.abcam.com/ps/products/222/ab222776/Images/ab222776-282653-anti-lc3b-antibody-rm293-western-blot.jpg "Western blot - Anti-LC3B antibody [RM293] (ab222776)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM293] to LC3B
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-LC3B antibody [RM293]
See all LC3B primary antibodies -
Description
Rabbit monoclonal [RM293] to LC3B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human LC3B (N terminal).
Database link: Q9GZQ8 -
Positive control
- WB: HeLa cell lysate, untreated or treated with chloroquine. IHC-P: Human brain tissue. ICC/IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab222776 was purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM293 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab222776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/100 - 1/200. Predicted molecular weight: 15 kDa. | |
| IHC-P | 1/100 - 1/200. | |
| ICC/IF | 1/100 - 1/200. |
Target
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Function
Probably involved in formation of autophagosomal vacuoles (autophagosomes). -
Tissue specificity
Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver. -
Sequence similarities
Belongs to the MAP1 LC3 family. -
Post-translational
modificationsThe precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II. -
Cellular localization
Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes. - Information by UniProt
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Database links
- Entrez Gene: 81631 Human
- Entrez Gene: 67443 Mouse
- Entrez Gene: 64862 Rat
- Omim: 609604 Human
- SwissProt: Q9GZQ8 Human
- SwissProt: Q9CQV6 Mouse
- SwissProt: Q62625 Rat
- Unigene: 356061 Human
see all -
Alternative names
- ATG8F antibody
- Autophagy-related protein LC3 B antibody
- Autophagy-related ubiquitin-like modifier LC3 B antibody
see all
Images
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Western blot - Anti-LC3B antibody [RM293] (ab222776)All lanes : Anti-LC3B antibody [RM293] (ab222776) at 1/200 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with chloroquine, cell lysate
Predicted band size: 15 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LC3B antibody [RM293] (ab222776)](https://www.abcam.com/ps/products/222/ab222776/Images/ab222776-282654-anti-lc3b-antibody-rm293-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LC3B antibody [RM293] (ab222776)Formalin-fixed, paraffin-embedded human brain tissue stained for LC3B with ab222776 at 1/200 dilution in immunohistochemical analysis.
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![Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody [RM293] (ab222776)](https://www.abcam.com/ps/products/222/ab222776/Images/ab222776-282655-anti-lc3b-antibody-rm293-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody [RM293] (ab222776)HeLa (human epithelial cell line from cervix adenocarcinoma) cells, untreated (left panel) or treated with chloroquine (right panel) stained for LC3B (red) using ab222776 at 1/200 dilution in ICC/IF.
Actin filaments were labeled with fluorescein phalloidin (green). Nuclei were labeled with DAPI (blue).
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![Anti-LC3B antibody [RM293] (ab222776)](https://www.abcam.com/ps/products/222/ab222776/Images/ab222776-4-benefits-of-recombinant-antibodies.png)
Anti-LC3B antibody [RM293] (ab222776)
Protocols
Datasheets and documents
References (0)
ab222776 has not yet been referenced specifically in any publications.
