Overview

  • Product name

  • Description

    Goat polyclonal to LC3C
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rhesus monkey, Common marmoset
  • Immunogen

    Synthetic peptide:

    SQETFGCLESAAPRD

    , corresponding to amino acids 121-135 of Human LC3C (NP_001004343.1).

  • Positive control

    • Human Cerebellum, Human Kidney

Properties

Applications

Our Abpromise guarantee covers the use of ab118501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3.75 - 7.5 µg/ml.

Target

  • Function

    Probably involved in formation of autophagosomal vacuoles (autophagosomes).
  • Tissue specificity

    Most abundant in placenta, lung and ovary.
  • Sequence similarities

    Belongs to the MAP1 LC3 family.
  • Post-translational
    modifications

    The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
  • Cellular localization

    Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATG8J antibody
    • Autophagy-related protein LC3 C antibody
    • Autophagy-related ubiquitin-like modifier LC3 C antibody
    • LC3 like protein 2 antibody
    • MAP1 light chain 3 like protein 2 antibody
    • MAP1 light chain 3 like protein 3 antibody
    • MAP1 light chain 3-like protein 3 antibody
    • MAP1A/MAP1B LC3 C antibody
    • MAP1A/MAP1B light chain 3 C antibody
    • MAP1LC3C antibody
    • Microtubule-associated protein 1 light chain 3 gamma antibody
    • Microtubule-associated proteins 1A/1B light chain 3C antibody
    • MLP3C_HUMAN antibody
    see all

Images

  • ab118501 at 3.75 µg/ml staining LC3C in Human Cerebellum by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
  • ab118501 at 3.75 µg/ml staining LC3C in Human Kidney by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.

References

ab118501 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

As stated on the datasheet, the immunogen of ab118501 is a synthetic peptide:
SQETFGCLESAAPRD , corresponding to amino acids 121-135 of Human LC3C (NP_001004343.1).

This sequence shows no homology LC3A isoform 1 and 2 and LC3B so therefore, ab118501 should not detect these proteins

Read More

Answer

Thank you for contacting us.
At this time, no cross-reactivity testing has been done for these products, although the immunogens show low homology with these proteins.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up