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Rabbit polyclonal to LC3C
Synthetic peptide conjugated to KLH, corresponding to amino acids 1-30 (MPPPQKIPSVRPFKQRKSLAIRQEEVAGIR) of Human LC3C (UniProt ID: Q9BXW4).
WB: 293, Ramos, HeLa, HepG2, T47D, and Jurkat cell lysates.
IHC: Human cancer tissue
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.09% Sodium azide
Constituent: 100% PBS
Concentration information loading...
Ammonium Sulphate Precipitation
ab168813 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: 17 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Probably involved in formation of autophagosomal vacuoles (autophagosomes).
Most abundant in placenta, lung and ovary.
Belongs to the MAP1 LC3 family.
The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
Information by UniProt
Autophagy-related protein LC3 C antibody
Autophagy-related ubiquitin-like modifier LC3 C antibody
Western blot - Anti-LC3C antibody (ab168813)
All lanes : Anti-LC3C antibody (ab168813) at 1/100 dilution Lane 1 : 293 lysate Lane 2 : Ramos lysate Lane 3 : HeLa lysate Lane 4 : HepG2 lysate Lane 5 : T47D lysate Lane 6 : Jurkat tissue lysate Lysates/proteins at 12.5 µg per lane. Predicted band size: 17 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LC3C antibody (ab168813)
Immunohistochemistry analysis of formalin-fixed and paraffin-embedded Human breast carcinoma tissue labeling LC3C with ab168813 at 1/50.
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