Key features and details
- Rabbit polyclonal to LC3C - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LC3C antibody - C-terminal
See all LC3C primary antibodies
DescriptionRabbit polyclonal to LC3C - C-terminal
Specificityab191574 is predicted to not cross-react with LC3A or LC3B.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human LC3C (C terminal). (18 amino acid peptide from near the carboxy terminus; NP_001004343).
Database link: Q9BXW4
- Human brain tissue lysate; Human brain tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab191574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 17 kDa).|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionProbably involved in formation of autophagosomal vacuoles (autophagosomes).
Tissue specificityMost abundant in placenta, lung and ovary.
Sequence similaritiesBelongs to the MAP1 LC3 family.
modificationsThe precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
Cellular localizationCytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
- Information by UniProt
- ATG8J antibody
- Autophagy-related protein LC3 C antibody
- Autophagy-related ubiquitin-like modifier LC3 C antibody
All lanes : Anti-LC3C antibody - C-terminal (ab191574) at 1 µg/ml
Lane 1 : Human brain tissue lysate
Lane 2 : Human brain tissue lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 17 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling LC3C with ab191574 at 5 µg/ml.
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling LC3C with ab191574 at 20 µg/ml.
ab191574 has not yet been referenced specifically in any publications.