Overview

  • Product name

    Anti-LC3C antibody - C-terminal
    See all LC3C primary antibodies
  • Description

    Rabbit polyclonal to LC3C - C-terminal
  • Host species

    Rabbit
  • Specificity

    ab191574 is predicted to not cross-react with LC3A or LC3B.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human LC3C (C terminal). (18 amino acid peptide from near the carboxy terminus; NP_001004343).
    Database link: Q9BXW4

  • Positive control

    • Human brain tissue lysate; Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab191574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 17 kDa).
IHC-P Use a concentration of 5 - 20 µg/ml.

Target

  • Function

    Probably involved in formation of autophagosomal vacuoles (autophagosomes).
  • Tissue specificity

    Most abundant in placenta, lung and ovary.
  • Sequence similarities

    Belongs to the MAP1 LC3 family.
  • Post-translational
    modifications

    The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
  • Cellular localization

    Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATG8J antibody
    • Autophagy-related protein LC3 C antibody
    • Autophagy-related ubiquitin-like modifier LC3 C antibody
    • LC3 like protein 2 antibody
    • MAP1 light chain 3 like protein 2 antibody
    • MAP1 light chain 3 like protein 3 antibody
    • MAP1 light chain 3-like protein 3 antibody
    • MAP1A/MAP1B LC3 C antibody
    • MAP1A/MAP1B light chain 3 C antibody
    • MAP1LC3C antibody
    • Microtubule-associated protein 1 light chain 3 gamma antibody
    • Microtubule-associated proteins 1A/1B light chain 3C antibody
    • MLP3C_HUMAN antibody
    see all

Images

  • All lanes : Anti-LC3C antibody - C-terminal (ab191574) at 1 µg/ml

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human brain tissue lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 17 kDa
    Observed band size: 16 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling LC3C with ab191574 at 5 µg/ml.

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling LC3C with ab191574 at 20 µg/ml.

References

ab191574 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab191574.
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