Product nameAnti-LC3C antibody [EPR8076]
See all LC3C primary antibodies
DescriptionRabbit monoclonal [EPR8076] to LC3C
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human LC3C.
- LC3C transfected 293T cell lysates, paraffin-embedded Human lung tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab167421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 17 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionProbably involved in formation of autophagosomal vacuoles (autophagosomes).
Tissue specificityMost abundant in placenta, lung and ovary.
Sequence similaritiesBelongs to the MAP1 LC3 family.
modificationsThe precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
Cellular localizationCytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
- Information by UniProt
- ATG8J antibody
- Autophagy-related protein LC3 C antibody
- Autophagy-related ubiquitin-like modifier LC3 C antibody
All lanes : Anti-LC3C antibody [EPR8076] (ab167421) at 1/10000 dilution
Lane 1 : LC3C transfected 293T cell lysate
Lane 2 : Mock transfected 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP antibody at 1/2000 dilution
Predicted band size: 17 kDa
Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling LC3C with ab167421 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab167421 has not yet been referenced specifically in any publications.