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    lc3c-antibody-epr8076-ab167421.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microtubules MT Associated Proteins MAP
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RecombinantRabMAb

Recombinant Anti-LC3C antibody [EPR8076] (ab167421)

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Western blot - Anti-LC3C antibody [EPR8076] (ab167421)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LC3C antibody [EPR8076] (ab167421)
  • Anti-LC3C antibody [EPR8076] (ab167421)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8076] to LC3C
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-LC3C antibody [EPR8076]
    See all LC3C primary antibodies
  • Description

    Rabbit monoclonal [EPR8076] to LC3C
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human LC3C.

  • Positive control

    • LC3C transfected 293T cell lysates, paraffin-embedded Human lung tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8076
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • MT Associated Proteins
    • MAP
    • Cancer
    • Signal transduction
    • Other
    • Cardiovascular
    • Heart
    • Autophagy
    • Autophagosome
    • Cardiovascular
    • Heart
    • Autophagy
    • APG gene products
    • Cancer
    • Signal transduction
    • Autophagy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • APG gene products
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • Autophagosome
    • Cancer
    • Cell Death
    • Autophagy
    • Signal Transduction
    • Cancer
    • Cell Death
    • Autophagy
    • APG gene products
    • Cancer
    • Cell Death
    • Autophagy
    • Autophagosome

Associated products

  • Alternative Versions

    • Anti-LC3C antibody [EPR8076] - BSA and Azide free (ab249433)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab167421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 17 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 17 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for ICC/IF or IP.

Target

  • Function

    Probably involved in formation of autophagosomal vacuoles (autophagosomes).
  • Tissue specificity

    Most abundant in placenta, lung and ovary.
  • Sequence similarities

    Belongs to the MAP1 LC3 family.
  • Post-translational
    modifications

    The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
  • Cellular localization

    Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
  • Target information above from: UniProt accession Q9BXW4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 440738 Human
    • Omim: 609605 Human
    • SwissProt: Q9BXW4 Human
    • Unigene: 534971 Human
    • Alternative names

      • ATG8J antibody
      • Autophagy-related protein LC3 C antibody
      • Autophagy-related ubiquitin-like modifier LC3 C antibody
      • LC3 like protein 2 antibody
      • MAP1 light chain 3 like protein 2 antibody
      • MAP1 light chain 3 like protein 3 antibody
      • MAP1 light chain 3-like protein 3 antibody
      • MAP1A/MAP1B LC3 C antibody
      • MAP1A/MAP1B light chain 3 C antibody
      • MAP1LC3C antibody
      • Microtubule-associated protein 1 light chain 3 gamma antibody
      • Microtubule-associated proteins 1A/1B light chain 3C antibody
      • MLP3C_HUMAN antibody
      see all

    Images

    • Western blot - Anti-LC3C antibody [EPR8076] (ab167421)
      Western blot - Anti-LC3C antibody [EPR8076] (ab167421)
      All lanes : Anti-LC3C antibody [EPR8076] (ab167421) at 1/10000 dilution

      Lane 1 : LC3C transfected 293T cell lysate
      Lane 2 : Mock transfected 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP antibody at 1/2000 dilution

      Predicted band size: 17 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LC3C antibody [EPR8076] (ab167421)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LC3C antibody [EPR8076] (ab167421)

      Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling LC3C with ab167421 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-LC3C antibody [EPR8076] (ab167421)
      Anti-LC3C antibody [EPR8076] (ab167421)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab167421? Please let us know so that we can cite the reference in this datasheet.

    ab167421 has not yet been referenced specifically in any publications.

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