Recombinant Anti-LC3C antibody [EPR8076] (ab167421)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-LC3C antibody [EPR8076]
See all LC3C primary antibodies -
Description
Rabbit monoclonal [EPR8076] to LC3C -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human LC3C.
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Positive control
- LC3C transfected 293T cell lysates, paraffin-embedded Human lung tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8076 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab167421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 17 kDa. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Probably involved in formation of autophagosomal vacuoles (autophagosomes). -
Tissue specificity
Most abundant in placenta, lung and ovary. -
Sequence similarities
Belongs to the MAP1 LC3 family. -
Post-translational
modificationsThe precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. -
Cellular localization
Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes. - Information by UniProt
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Database links
- Entrez Gene: 440738 Human
- Omim: 609605 Human
- SwissProt: Q9BXW4 Human
- Unigene: 534971 Human
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Alternative names
- ATG8J antibody
- Autophagy-related protein LC3 C antibody
- Autophagy-related ubiquitin-like modifier LC3 C antibody
see all
Images
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![Western blot - Anti-LC3C antibody [EPR8076] (ab167421)](https://www.abcam.com/ps/products/167/ab167421/Images/LC3C-Primary-antibodies-ab167421-1.jpg)
Western blot - Anti-LC3C antibody [EPR8076] (ab167421)All lanes : Anti-LC3C antibody [EPR8076] (ab167421) at 1/10000 dilution
Lane 1 : LC3C transfected 293T cell lysate
Lane 2 : Mock transfected 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP antibody at 1/2000 dilution
Predicted band size: 17 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LC3C antibody [EPR8076] (ab167421)](https://www.abcam.com/ps/products/167/ab167421/Images/LC3C-Primary-antibodies-ab167421-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LC3C antibody [EPR8076] (ab167421)Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling LC3C with ab167421 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Datasheets and documents
References
ab167421 has not yet been referenced specifically in any publications.
