Overview

  • Product name
    Anti-LC3C antibody [EPR8076]
    See all LC3C primary antibodies
  • Description
    Rabbit monoclonal [EPR8076] to LC3C
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human LC3C (UniProt: Q9BXW4)

  • Positive control
    • LC3C transfected 293T cell lysates, paraffin-embedded Human lung tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab167421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 17 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function
      Probably involved in formation of autophagosomal vacuoles (autophagosomes).
    • Tissue specificity
      Most abundant in placenta, lung and ovary.
    • Sequence similarities
      Belongs to the MAP1 LC3 family.
    • Post-translational
      modifications
      The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
    • Cellular localization
      Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATG8J antibody
      • Autophagy-related protein LC3 C antibody
      • Autophagy-related ubiquitin-like modifier LC3 C antibody
      • LC3 like protein 2 antibody
      • MAP1 light chain 3 like protein 2 antibody
      • MAP1 light chain 3 like protein 3 antibody
      • MAP1 light chain 3-like protein 3 antibody
      • MAP1A/MAP1B LC3 C antibody
      • MAP1A/MAP1B light chain 3 C antibody
      • MAP1LC3C antibody
      • Microtubule-associated protein 1 light chain 3 gamma antibody
      • Microtubule-associated proteins 1A/1B light chain 3C antibody
      • MLP3C_HUMAN antibody
      see all

    Images

    • All lanes : Anti-LC3C antibody [EPR8076] (ab167421) at 1/10000 dilution

      Lane 1 : LC3C transfected 293T cell lysate
      Lane 2 : Mock transfected 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP antibody at 1/2000 dilution

      Predicted band size: 17 kDa

    • Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling LC3C with ab167421 at 1/100 dilution.

    References

    ab167421 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab167421.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up