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Rabbit monoclonal [EPR8076] to LC3C
IHC-P more details Unsuitable for:
ICC/IF or IP
Synthetic peptide corresponding to residues in Human LC3C (UniProt: Q9BXW4)
LC3C transfected 293T cell lysates, paraffin-embedded Human lung tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Predicted molecular weight: 17 kDa.
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Is unsuitable for ICC/IF or IP.
Probably involved in formation of autophagosomal vacuoles (autophagosomes).
Most abundant in placenta, lung and ovary.
Belongs to the MAP1 LC3 family.
The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
Information by UniProt
Autophagy-related protein LC3 C antibody
Autophagy-related ubiquitin-like modifier LC3 C antibody
Western blot - Anti-LC3C antibody [EPR8076] (ab167421)
All lanes : Anti-LC3C antibody [EPR8076] (ab167421) at 1/10000 dilution Lane 1 : LC3C transfected 293T cell lysate Lane 2 : Mock transfected 293T cell lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : Goat anti-rabbit HRP antibody at 1/2000 dilution Predicted band size: 17 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LC3C antibody [EPR8076] (ab167421)
Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling LC3C with ab167421 at 1/100 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"