Recombinant
RabMAb

Anti-LC3C antibody [EPR8076] (ab167421)

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Overview

  • Product name
    Anti-LC3C antibody [EPR8076]
    See all LC3C primary antibodies
  • Description
    Rabbit monoclonal [EPR8076] to LC3C
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human LC3C (UniProt: Q9BXW4)

  • Positive control
    • LC3C transfected 293T cell lysates, paraffin-embedded Human lung tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab167421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 17 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF or IP.

    • Target

    • Function
      Probably involved in formation of autophagosomal vacuoles (autophagosomes).
    • Tissue specificity
      Most abundant in placenta, lung and ovary.
    • Sequence similarities
      Belongs to the MAP1 LC3 family.
    • Post-translational
      modifications
      The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
    • Cellular localization
      Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
    • Information by UniProt
    • Database links
      • Alternative names
        • ATG8J antibody
        • Autophagy-related protein LC3 C antibody
        • Autophagy-related ubiquitin-like modifier LC3 C antibody
        • LC3 like protein 2 antibody
        • MAP1 light chain 3 like protein 2 antibody
        • MAP1 light chain 3 like protein 3 antibody
        • MAP1 light chain 3-like protein 3 antibody
        • MAP1A/MAP1B LC3 C antibody
        • MAP1A/MAP1B light chain 3 C antibody
        • MAP1LC3C antibody
        • Microtubule-associated protein 1 light chain 3 gamma antibody
        • Microtubule-associated proteins 1A/1B light chain 3C antibody
        • MLP3C_HUMAN antibody
        see all

      Images

      • Western blot - Anti-LC3C antibody [EPR8076] (ab167421)
        Western blot - Anti-LC3C antibody [EPR8076] (ab167421)
        All lanes : Anti-LC3C antibody [EPR8076] (ab167421) at 1/10000 dilution

        Lane 1 : LC3C transfected 293T cell lysate
        Lane 2 : Mock transfected 293T cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP antibody at 1/2000 dilution

        Predicted band size: 17 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LC3C antibody [EPR8076] (ab167421)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LC3C antibody [EPR8076] (ab167421)
        Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling LC3C with ab167421 at 1/100 dilution.

      References

      ab167421 has not yet been referenced specifically in any publications.

      Publishing research using ab167421? Please let us know so that we can cite the reference in this datasheet.

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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