Overview

  • Product name

    Anti-LC3C antibody [EPR8077]
    See all LC3C primary antibodies
  • Description

    Rabbit monoclonal [EPR8077] to LC3C
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human LC3C aa 100-200. The exact sequence is proprietary.

  • Positive control

    • LC3C transfected 293T cell lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab150367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 17 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Probably involved in formation of autophagosomal vacuoles (autophagosomes).
    • Tissue specificity

      Most abundant in placenta, lung and ovary.
    • Sequence similarities

      Belongs to the MAP1 LC3 family.
    • Post-translational
      modifications

      The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
    • Cellular localization

      Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
    • Information by UniProt
    • Database links

    • Alternative names

      • ATG8J antibody
      • Autophagy-related protein LC3 C antibody
      • Autophagy-related ubiquitin-like modifier LC3 C antibody
      • LC3 like protein 2 antibody
      • MAP1 light chain 3 like protein 2 antibody
      • MAP1 light chain 3 like protein 3 antibody
      • MAP1 light chain 3-like protein 3 antibody
      • MAP1A/MAP1B LC3 C antibody
      • MAP1A/MAP1B light chain 3 C antibody
      • MAP1LC3C antibody
      • Microtubule-associated protein 1 light chain 3 gamma antibody
      • Microtubule-associated proteins 1A/1B light chain 3C antibody
      • MLP3C_HUMAN antibody
      see all

    Images

    • All lanes : Anti-LC3C antibody [EPR8077] (ab150367) at 1/10000 dilution

      Lane 1 : LC3C transfected 293T cell lysates
      Lane 2 : Mock-transfected 293T cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 17 kDa

    References

    This product has been referenced in:

    • Pascall JC  et al. The immune system GTPase GIMAP6 interacts with the Atg8 homologue GABARAPL2 and is recruited to autophagosomes. PLoS One 8:e77782 (2013). Read more (PubMed: 24204963) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab150367.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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