Overview

  • Product name

  • Description

    Goat polyclonal to LCAT
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Baboon
  • Immunogen

    Synthetic peptide corresponding to Human LCAT aa 366-378 (internal sequence) (Cysteine residue). (NP_000220.1).
    Sequence:

    C-EDGDDTVATRSTE


    Database link: P04180

  • Positive control

    • WB: Human testis lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab223574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa).

Target

  • Function

    Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms.
  • Tissue specificity

    Expressed mainly in brain, liver and testes. Secreted into plasma and cerebral spinal fluid. In liver, expressed in HEPG2 hepatocytes.
  • Involvement in disease

    Defects in LCAT are the cause of lecithin-cholesterol acyltransferase deficiency (LCATD) [MIM:245900]; also called Norum disease. LCATD is a disorder of lipoprotein metabolism characterized by inadequate esterification of plasmatic cholesterol. Two clinical forms are recognized: familial LCAT deficiency and fish-eye disease. Familial LCAT deficiency is associated with a complete absence of alpha and beta LCAT activities and results in esterification anomalies involving both HDL (alpha-LCAT activity) and LDL (beta-LCAT activity). It causes a typical triad of diffuse corneal opacities, target cell hemolytic anemia, and proteinuria with renal failure.
    Defects in LCAT are a cause of fish-eye disease (FED) [MIM:136120]; also known as dyslipoproteinemic corneal dystrophy or alpha-LCAT deficiency. FED is due to a partial LCAT deficiency that affects only alpha-LCAT activity. It is characterized by low plasma HDL and corneal opacities due to accumulation of cholesterol deposits in the cornea ('fish-eye').
  • Sequence similarities

    Belongs to the AB hydrolase superfamily. Lipase family.
  • Post-translational
    modifications

    O- and N-glycosylated. O-glycosylation on Thr-431 and Ser-433 consists of sialylated galactose beta 1-->3N-acetylgalactosamine structures. N-glycosylated sites contain sialylated triantennary and/or biantennary complex structures.
  • Cellular localization

    Secreted. Secreted into blood plasma. Produced in astrocytes and secreted into cerebral spinal fluid.
  • Information by UniProt
  • Database links

  • Alternative names

    • LCAT antibody
    • LCAT_HUMAN antibody
    • Lecithin cholesterol acyltransferase antibody
    • Lecithin-cholesterol acyltransferase antibody
    • Phosphatidylcholine sterol acyltransferase antibody
    • Phosphatidylcholine-sterol acyltransferase antibody
    • Phospholipid cholesterol acyltransferase antibody
    • Phospholipid-cholesterol acyltransferase antibody
    see all

Images

  • Anti-LCAT antibody (ab223574) at 1 µg/ml + Human testis lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 59 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

ab223574 has not yet been referenced specifically in any publications.

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