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RecombinantRabMAb

Recombinant Anti-LCAT antibody [EPR1383(2)] - BSA and Azide free (ab175019)

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  • SDS
  • Certificate of Compliance
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Western blot - Anti-LCAT antibody [EPR1383(2)] - BSA and Azide free (ab175019)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LCAT antibody [EPR1383(2)] - BSA and Azide free (ab175019)
  • Anti-LCAT antibody [EPR1383(2)] - BSA and Azide free (ab175019)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1383(2)] to LCAT - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

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View more associated products

Overview

  • Product name

    Anti-LCAT antibody [EPR1383(2)] - BSA and Azide free
    See all LCAT primary antibodies
  • Description

    Rabbit monoclonal [EPR1383(2)] to LCAT - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab175019 is the carrier-free version of ab109417. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab175019 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1383(2)
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Alternative Versions

    • Anti-LCAT antibody [EPR1383(2)] (ab109417)
  • Conjugation kits

    • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Recombinant Protein

    • Recombinant Human LCAT protein (ab104359)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab175019 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 50 kDa.
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 50 kDa.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms.
    • Tissue specificity

      Expressed mainly in brain, liver and testes. Secreted into plasma and cerebral spinal fluid. In liver, expressed in HEPG2 hepatocytes.
    • Involvement in disease

      Defects in LCAT are the cause of lecithin-cholesterol acyltransferase deficiency (LCATD) [MIM:245900]; also called Norum disease. LCATD is a disorder of lipoprotein metabolism characterized by inadequate esterification of plasmatic cholesterol. Two clinical forms are recognized: familial LCAT deficiency and fish-eye disease. Familial LCAT deficiency is associated with a complete absence of alpha and beta LCAT activities and results in esterification anomalies involving both HDL (alpha-LCAT activity) and LDL (beta-LCAT activity). It causes a typical triad of diffuse corneal opacities, target cell hemolytic anemia, and proteinuria with renal failure.
      Defects in LCAT are a cause of fish-eye disease (FED) [MIM:136120]; also known as dyslipoproteinemic corneal dystrophy or alpha-LCAT deficiency. FED is due to a partial LCAT deficiency that affects only alpha-LCAT activity. It is characterized by low plasma HDL and corneal opacities due to accumulation of cholesterol deposits in the cornea ('fish-eye').
    • Sequence similarities

      Belongs to the AB hydrolase superfamily. Lipase family.
    • Post-translational
      modifications

      O- and N-glycosylated. O-glycosylation on Thr-431 and Ser-433 consists of sialylated galactose beta 1-->3N-acetylgalactosamine structures. N-glycosylated sites contain sialylated triantennary and/or biantennary complex structures.
    • Cellular localization

      Secreted. Secreted into blood plasma. Produced in astrocytes and secreted into cerebral spinal fluid.
    • Target information above from: UniProt accession P04180 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3931 Human
      • Entrez Gene: 16816 Mouse
      • Entrez Gene: 24530 Rat
      • Omim: 606967 Human
      • SwissProt: P04180 Human
      • SwissProt: P16301 Mouse
      • SwissProt: P18424 Rat
      • Unigene: 387239 Human
      • Unigene: 1593 Mouse
      • Unigene: 10481 Rat
      see all
    • Alternative names

      • LCAT antibody
      • LCAT_HUMAN antibody
      • Lecithin cholesterol acyltransferase antibody
      • Lecithin-cholesterol acyltransferase antibody
      • Phosphatidylcholine sterol acyltransferase antibody
      • Phosphatidylcholine-sterol acyltransferase antibody
      • Phospholipid cholesterol acyltransferase antibody
      • Phospholipid-cholesterol acyltransferase antibody
      see all

    Images

    • Western blot - Anti-LCAT antibody [EPR1383(2)] - BSA and Azide free (ab175019)
      Western blot - Anti-LCAT antibody [EPR1383(2)] - BSA and Azide free (ab175019)
      All lanes : Anti-LCAT antibody [EPR1383(2)] (ab109417) at 1/1000 dilution

      Lane 1 : Human serum lysate
      Lane 2 : Human plasma lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 50 kDa



      This data was developed using ab109417, the same antibody clone in a different buffer formulation.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LCAT antibody [EPR1383(2)] - BSA and Azide free (ab175019)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LCAT antibody [EPR1383(2)] - BSA and Azide free (ab175019)
      This data was developed using ab109417, the same antibody clone in a different buffer formulation.ab109417, at 1/250 dilution, staining LCAT in paraffin-embedded Human liver tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    • Anti-LCAT antibody [EPR1383(2)] - BSA and Azide free (ab175019)
      Anti-LCAT antibody [EPR1383(2)] - BSA and Azide free (ab175019)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab175019? Please let us know so that we can cite the reference in this datasheet.

    ab175019 has not yet been referenced specifically in any publications.

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