Anti-LCLAT1 antibody (ab153987)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-LCLAT1 antibody
See all LCLAT1 primary antibodies -
Description
Rabbit polyclonal to LCLAT1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Pig
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Immunogen
A recombinant fragment corresponding to a region within amino acids 193 and 414 of Human LCLAT1 (Uniprot ID Q6UWP7).
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Positive control
- Hela membrane and whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78% PBS, 1% BSA, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab153987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 49 kDa. |
Target
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Function
Acyl-CoA:lysocardiolipin acyltransferase. Possesses both lysophosphatidylinositol acyltransferase (LPIAT) and lysophosphatidylglycerol acyltransferase (LPGAT) activities. Recognizes both monolysocardiolipin and dilysocardiolipin as substrates with a preference for linoleoyl-CoA and oleoyl-CoA as acyl donors. Acts as a remodeling enzyme for cardiolipin, a major membrane polyglycerophospholipid. Converts lysophosphatidic acid (LPA) into phosphatidic acid (PA) with a relatively low activity. Required for establishment of the hematopoietic and endothelial lineages. -
Tissue specificity
Expressed at higher level in heart, kidney and pancreas than in brain, spleen, liver, lung, small intestine and placenta. -
Pathway
Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3. -
Sequence similarities
Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. -
Domain
The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 253558 Human
- Entrez Gene: 225010 Mouse
- Entrez Gene: 100217396 Pig
- Omim: 614241 Human
- SwissProt: Q6UWP7 Human
- SwissProt: Q3UN02 Mouse
- Unigene: 468048 Human
- Unigene: 662770 Human
see all -
Alternative names
- 1-acylglycerol-3-phosphate O-acyltransferase 8 antibody
- 1-AGP acyltransferase 8 antibody
- 1-AGPAT 8 antibody
see all
Images
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Western blot - Anti-LCLAT1 antibody (ab153987)All lanes : Anti-LCLAT1 antibody (ab153987) at 1/500 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa membrane lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 49 kDa
10% SDS PAGE
Protocols
References
ab153987 has not yet been referenced specifically in any publications.
