Key features and details
- Rabbit polyclonal to LCMT2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-LCMT2 antibody
DescriptionRabbit polyclonal to LCMT2
Specificityab112562 is predicted to not cross-react with other LCMT protein family members. At least two isoforms of LCMT2 are known to exist; this antibody will detect only the long isoform.
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (16 amino acids) near the N terminal of Human LCMT2.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab112562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 76 kDa.|
|ICC/IF||Use a concentration of 20 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
FunctionProbable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA (By similarity). May methylate the carboxyl group of leucine residues to form alpha-leucine ester residues.
PathwaytRNA modification; wybutosine-tRNA(Phe) biosynthesis.
Sequence similaritiesBelongs to the methyltransferase superfamily. LCMT family.
- Information by UniProt
- KIAA0547 antibody
- LCMT 2 antibody
- Lcmt2 antibody
Immunofluorescence of LCMT2 in mouse brain tissue with ab112562 at 20 ug/mL.
All lanes : Anti-LCMT2 antibody (ab112562) at 1 µg/ml
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat brain tissue lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 76 kDa
Immunohistochemistry of LCMT2 in mouse brain tissue with ab112562 at 5 ug/mL.
ab112562 has not yet been referenced specifically in any publications.