Anti-LCN12 antibody (ab231146)
Key features and details
- Rabbit polyclonal to LCN12
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-LCN12 antibody -
Description
Rabbit polyclonal to LCN12 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment (His-tag) corresponding to Human LCN12 aa 50 to the C-terminus. (N-terminal His-tag and GST-tag. Expressed in E.coli).
Database link: Q6JVE5 -
Positive control
- WB: Recombinant human LCN12 protein; Mouse testis lysate. IHC-P: Human kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab231146 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 22 kDa.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 22 kDa. |
Target
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Function
Binds all-trans retinoic acid and may act as a retinoid carrier protein within the epididymis. May play a role in male fertility. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 286256 Human
- Entrez Gene: 77701 Mouse
- Omim: 612905 Human
- SwissProt: Q6JVE5 Human
- SwissProt: 77701 Mouse
- SwissProt: Q2TA58 Mouse
- SwissProt: Q6JVL5 Mouse
- Unigene: 440519 Human
see all -
Alternative names
- Epididymal-specific lipocalin-12 antibody
- LCN12 antibody
- LCN12_HUMAN antibody
see all
Images
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Anti-LCN12 antibody (ab231146) at 2 µg/ml + Recombinant human LCN12 protein
Predicted band size: 22 kDa -
Anti-LCN12 antibody (ab231146) at 3 µg/ml + Mouse testis lysate
Secondary
HRP-Linked Guinea pig anti-Rabbit at 1/1000 dilution
Predicted band size: 22 kDa -
Formalin-fixed, paraffin-embedded human kidney tissue stained for LCN12 with ab231146 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231146 has not yet been referenced specifically in any publications.