Key features and details
- Rabbit polyclonal to LDB1
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-LDB1 antibody
See all LDB1 primary antibodies
DescriptionRabbit polyclonal to LDB1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human, Pig
Predicted to work with: Mouse, Chicken
Recombinant fragment (His-T7-tag) corresponding to Human LDB1 aa 112-411. (Expressed in E.coli).
DGPKRYTIGRTLIPRYFRSIFEGGATELYYVLKHPKEAFHSNFVSLDCDQ GSMVTQHGKPMFTQVCVEGRLYLEFMFDDMMRIKTWHFSIRQHRELIPRS ILAMHAQDPQMLDQLSKNITRCGLSNSTLNYLRLCVILEPMQELMSRHKT YSLSPRDCLKTCLFQKWQRMVAPPAEPTRQQPSKRRKRKMSGGSTMSSGG GNTNNSNSKKKSPASTFALSSQVPDVMVVGEPTLMGGEFGDEDERLITRL ENTQFDAANGIDDEDSFNNSPALGANSPWNSKPPSSQESKSENPTSQASQ
Database link: Q86U70
- WB: Jurkat cell lysate; Pig kidney lysate; Recombinant human LDB1 protein. IHC-P: Human brain tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 47 kDa.|
FunctionBinds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. May play a role in the development of motor neurons. Acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. Acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state.
Tissue specificityExpressed in a wide range of adult tissues including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
Sequence similaritiesBelongs to the LDB family.
DomainThe dimerization domain is located in the N-terminus.
modificationsUbiquitinated by RLIM/RNF12, leading to its degradation by the proteasome.
- Information by UniProt
- Carboxyl Terminal LIM Domain Binding 2 antibody
- Carboxyl-terminal LIM domain-binding protein 2 antibody
- CLIM 2 antibody
Formalin-fixed, paraffin-embedded human brain tissue stained for LDB1 using ab233213 at 20 µg/mL in immunohistochemical analysis. DAB staining.
All lanes : Anti-LDB1 antibody (ab233213) at 1 µg/ml
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : Pig kidney tissue
All lanes : HRP-Linked Guinea pig anti-rabbit at 1/1000 dilution
Predicted band size: 47 kDa
Anti-LDB1 antibody (ab233213) at 2 µg/ml + Recombinant human LDB1 protein
Predicted band size: 47 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233213 has not yet been referenced specifically in any publications.