- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-LDB2 antibody
See all LDB2 primary antibodies -
DescriptionRabbit polyclonal to LDB2
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Host speciesRabbit
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SpecificityThis antibody detects a single clean band representing LDB2 at 42kD in human kidney and mouse spleen.
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Tested applicationsSuitable for: ICC/IF, WBmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Chicken
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Immunogen
Synthetic peptide:
SKESYHNSSITVDC
conjugated to KLH, corresponding to amino acids 107-120 of Human LDB2.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab3627 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | ||
| WB |
Application notes
ICC/IF: Use at an assay dependent dilution (PMID 19675209).
WB: 1/500. Detects a band of approximately 42 kDa (predicted molecular weight: 42.7 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: 1/500. Detects a band of approximately 42 kDa (predicted molecular weight: 42.7 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionBinds to the LIM domain of a wide variety of LIM domain-containing transcription factors.
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Sequence similaritiesBelongs to the LDB family.
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Post-translational
modificationsUbiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 395631 Chicken
- Entrez Gene: 9079 Human
- Entrez Gene: 16826 Mouse
- Omim: 603450 Human
- SwissProt: Q9W676 Chicken
- SwissProt: O43679 Human
- SwissProt: O55203 Mouse
- Unigene: 714330 Human
see all -
Alternative names
- Carboxyl terminal LIM domain binding protein 1 antibody
- Carboxyl terminal LIM domain protein 1 antibody
- Carboxyl-terminal LIM domain-binding protein 1 antibody
see all
Images
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Western blot - Anti-LDB2 antibody (ab3627)Western blot using ab3627 at 1/500.
Lane 1: Human Kidney
Lane 2: Mouse SpleenLysates at 18
µ g per lane.
Secondary ab: Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed
ab7090 (1/5000).
Exposure time: 1 minute.
References
This product has been referenced in:
- Storbeck CJ et al. The Ldb1 and Ldb2 transcriptional cofactors interact with the Ste20-like kinase SLK and regulate cell migration. Mol Biol Cell 20:4174-82 (2009). ICC/IF ; Mouse . Read more (PubMed: 19675209) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"