Overview

  • Product name
    Anti-LDB2 antibody
    See all LDB2 primary antibodies
  • Description
    Rabbit polyclonal to LDB2
  • Host species
    Rabbit
  • Specificity
    This antibody detects a single clean band representing LDB2 at 42kD in human kidney and mouse spleen.
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide:

    SKESYHNSSITVDC

    conjugated to KLH, corresponding to amino acids 107-120 of Human LDB2.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
  • Application notes
    ICC/IF: Use at an assay dependent dilution (PMID 19675209).
    WB: 1/500. Detects a band of approximately 42 kDa (predicted molecular weight: 42.7 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function
      Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors.
    • Sequence similarities
      Belongs to the LDB family.
    • Post-translational
      modifications
      Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • Carboxyl terminal LIM domain binding protein 1 antibody
      • Carboxyl terminal LIM domain protein 1 antibody
      • Carboxyl-terminal LIM domain-binding protein 1 antibody
      • CLIM 1 antibody
      • CLIM-1 antibody
      • CLIM1 antibody
      • LDB 1 antibody
      • LDB 2 antibody
      • LDB-2 antibody
      • LDB1 antibody
      • LDB2 antibody
      • LDB2_HUMAN antibody
      • LIM binding domain 2 antibody
      • LIM domain binding 2 antibody
      • LIM domain binding factor 2 antibody
      • LIM domain binding factor CLIM 1 antibody
      • LIM domain binding factor CLIM1 antibody
      • LIM domain binding protein 2 antibody
      • LIM domain-binding factor CLIM1 antibody
      • LIM domain-binding protein 2 antibody
      • OTTHUMP00000158632 antibody
      • OTTHUMP00000217823 antibody
      • OTTHUMP00000217824 antibody
      • OTTHUMP00000217825 antibody
      • OTTHUMP00000217827 antibody
      see all

    Images

    • Western blot - Anti-LDB2 antibody (ab3627)
      Western blot - Anti-LDB2 antibody (ab3627)

      Western blot using ab3627 at 1/500.

      Lane 1: Human Kidney
      Lane 2: Mouse Spleen

      Lysates at 18µg per lane.
      Secondary ab: Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed
      ab7090 (1/5000).
      Exposure time: 1 minute.

    References

    This product has been referenced in:
    • Storbeck CJ  et al. The Ldb1 and Ldb2 transcriptional cofactors interact with the Ste20-like kinase SLK and regulate cell migration. Mol Biol Cell 20:4174-82 (2009). ICC/IF ; Mouse . Read more (PubMed: 19675209) »

    See 1 Publication for this product

    Publishing research using ab3627? Please let us know so that we can cite the reference in this datasheet.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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