For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    ldb2-antibody-ab3627.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling Transcription Other factors
Share by email

Anti-LDB2 antibody (ab3627)

  • Datasheet
Reviews (1)Q&A (1)References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-LDB2 antibody (ab3627)

    Key features and details

    • Rabbit polyclonal to LDB2
    • Suitable for: WB
    • Reacts with: Mouse, Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-LDB2 antibody
      See all LDB2 primary antibodies
    • Description

      Rabbit polyclonal to LDB2
    • Host species

      Rabbit
    • Specificity

      This antibody detects a single clean band representing LDB2 at 42kD in human kidney and mouse spleen.
    • Tested Applications & Species

      Application Species
      WB
      Mouse
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide:

      SKESYHNSSITVDC

      conjugated to KLH, corresponding to amino acids 107-120 of Human LDB2.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.01% Sodium azide
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Other factors

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab3627 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Mouse
    Human
    All applications
    Chicken
    Application Abreviews Notes
    WB
    Notes
  • Application notes
    ICC/IF: Use at an assay dependent dilution (PMID 19675209).
    WB: 1/500. Detects a band of approximately 42 kDa (predicted molecular weight: 42.7 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors.
    • Sequence similarities

      Belongs to the LDB family.
    • Post-translational
      modifications

      Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession O43679 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 395631 Chicken
      • Entrez Gene: 9079 Human
      • Entrez Gene: 16826 Mouse
      • Omim: 603450 Human
      • SwissProt: Q9W676 Chicken
      • SwissProt: O43679 Human
      • SwissProt: O55203 Mouse
      • Unigene: 714330 Human
      • Unigene: 25785 Mouse
      see all
    • Alternative names

      • Carboxyl terminal LIM domain binding protein 1 antibody
      • Carboxyl terminal LIM domain protein 1 antibody
      • Carboxyl-terminal LIM domain-binding protein 1 antibody
      • CLIM 1 antibody
      • CLIM-1 antibody
      • CLIM1 antibody
      • LDB 1 antibody
      • LDB 2 antibody
      • LDB-2 antibody
      • LDB1 antibody
      • LDB2 antibody
      • LDB2_HUMAN antibody
      • LIM binding domain 2 antibody
      • LIM domain binding 2 antibody
      • LIM domain binding factor 2 antibody
      • LIM domain binding factor CLIM 1 antibody
      • LIM domain binding factor CLIM1 antibody
      • LIM domain binding protein 2 antibody
      • LIM domain-binding factor CLIM1 antibody
      • LIM domain-binding protein 2 antibody
      • OTTHUMP00000158632 antibody
      • OTTHUMP00000217823 antibody
      • OTTHUMP00000217824 antibody
      • OTTHUMP00000217825 antibody
      • OTTHUMP00000217827 antibody
      see all

    Images

    • Western blot - Anti-LDB2 antibody (ab3627)
      Western blot - Anti-LDB2 antibody (ab3627)

      Western blot using ab3627 at 1/500.

      Lane 1: Human Kidney
      Lane 2: Mouse Spleen

      Lysates at 18µg per lane.
      Secondary ab: Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed
      ab7090 (1/5000).
      Exposure time: 1 minute.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab3627? Please let us know so that we can cite the reference in this datasheet.

    ab3627 has been referenced in 1 publication.

    • Storbeck CJ  et al. The Ldb1 and Ldb2 transcriptional cofactors interact with the Ste20-like kinase SLK and regulate cell migration. Mol Biol Cell 20:4174-82 (2009). ICC/IF ; Mouse . PubMed: 19675209

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-LDB2 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Embryonic spinal cord)
    Permeabilization
    Yes - 0.1%Triton X-100
    Specification
    Embryonic spinal cord
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Frederic Clotman

    Verified customer

    Submitted Aug 20 2018

    Question

    Do you have the peptide available for this antibody?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 21 2004

    Answer

    Thank you for your enquiry. Unfortunately the peptide is not available for purchasing. If you have any further questions, please let us know.

    Read More

    Abcam Scientific Support

    Answered on Dec 21 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.