• Product name

  • Description

    Goat polyclonal to LDB3
  • Host species

  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Pig
  • Immunogen

    Synthetic peptide:


    , corresponding to Internal sequence amino acids 251-262 of Human LDB3


  • Form

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab40840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 - 6 µg/ml.
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 77 kDa.


  • Function

    May function as an adapter in striated muscle to couple protein kinase C-mediated signaling via its LIM domains to the cytoskeleton.
  • Tissue specificity

    Expressed primarily in skeletal muscle and to a lesser extent in heart. Also detected in brain and placenta.
  • Involvement in disease

    Defects in LDB3 are the cause of cardiomyopathy dilated type 1C (CMD1C) [MIM:601493]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in LDB3 are the cause of left ventricular non-compaction type 3 (LVNC3) [MIM:601493]. Left ventricular non-compaction is characterized by numerous prominent trabeculations and deep intertrabecular recesses in hypertrophied and hypokinetic segments of the left ventricle.
    Defects in LDB3 are the cause of myopathy myofibrillar ZASP-related (MFM-ZASP) [MIM:609452]. A neuromuscular disorder characterized by distal and proximal muscle weakness with signs of cardiomyopathy and neuropathy.
  • Sequence similarities

    Contains 3 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
  • Cellular localization

    Cytoplasm > perinuclear region. Cell projection > pseudopodium. Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Localized to the cytoplasm around nuclei and pseudopodia of undifferentiated cells and detected throughout the myotubes of differentiated cells. Colocalizes with ACTN2 at the Z-lines.
  • Information by UniProt
  • Database links

  • Alternative names

    • CMD1C antibody
    • CYPHER antibody
    • HGNC:15710 antibody
    • KIAA01613 antibody
    • KIAA0613 antibody
    • Ldb3 antibody
    • LDB3_HUMAN antibody
    • LDB3Z1 antibody
    • LDB3Z4 antibody
    • LIM domain binding 3 antibody
    • LIM domain binding protein 3 antibody
    • LIM domain-binding protein 3 antibody
    • LVNC3 antibody
    • ORACLE antibody
    • PDLIM6 antibody
    • PDZ and LIM domain 6 antibody
    • Protein cypher antibody
    • Z band alternatively spliced PDZ motif antibody
    • Z band alternatively spliced PDZ motif protein antibody
    • Z-band alternatively spliced PDZ-motif protein antibody
    • ZASP antibody
    see all


  • Anti-LDB3 antibody (ab40840) at 0.01 µg/ml + Human Muscle lysate (35µg protein in RIPA buffer).

    Predicted band size: 77 kDa

    Primary incubation was 1 hour. Detected by chemiluminescence.

    Experiments gave bands at approx 80-90kDa and 30-35kDa in Human Muscle lysates after 0.01µg/ml antibody staining. These bands correspond to earlier findings in literature (Huang et al J Biol Chem. 2003 Feb 28;278(9):7360-5; PMID: 12499364).

  • ab40840 at 3.8 µg/ml staining LBD3 in Human Skeletal muscle by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate pH 6, AP-staining.


This product has been referenced in:

  • Ulbricht A  et al. Induction and adaptation of chaperone-assisted selective autophagy CASA in response to resistance exercise in human skeletal muscle. Autophagy 11:538-46 (2015). WB . Read more (PubMed: 25714469) »
  • Johnson EK  et al. Identification of new dystroglycan complexes in skeletal muscle. PLoS One 8:e73224 (2013). Mouse . Read more (PubMed: 23951345) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Thank you again for your call yesterday and for letting us know about the trouble with the new lot of this antibody.
I've heard back from the lab, and the new batch of ab40840 passed QC testing, however it did need to be used at a much higher concentration. With the previous batch, they used between 0.01 and 0.03 ug/mL of antibody in Western blot, and the new batch requires 0.3-1 ug/mL. You current dilution already falls in this range (1:500 or 1 ug/mL), however in light of this QC data I would recommend increasing the concentration or using a more sensitive detection system if possible. Unfortunately the lab does not have a different lot to try and does not expect to produce a new lot soon.
I am very sorry for this lot-to-lot variability and for the inconvenience. If you would prefer a credit or refund at this time, I can set that up for you promptly. Please let me know if you have any questions or if there is anything else that we can do for you.

Read More


Thank you for your call today and for letting us know about the trouble with this antibody.
As we discussed, I'm sending a free of charge replacement vial on the order ***, which should arrive tomorrow. I've also asked the lab if they are producing a new lot, just in case this new vial also does not work well.
Please keep me updated about the results with this new lot, and let me know if there is anything else that we can do for you.

Read More

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