Product nameAnti-LDB3 antibody [EPR10126]
See all LDB3 primary antibodies
DescriptionRabbit monoclonal [EPR10126] to LDB3
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human LDB3 aa 50-150 (N terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: O75112
- A431 and HT 1080 cell line lysates
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab171936 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 77 kDa.|
FunctionMay function as an adapter in striated muscle to couple protein kinase C-mediated signaling via its LIM domains to the cytoskeleton.
Tissue specificityExpressed primarily in skeletal muscle and to a lesser extent in heart. Also detected in brain and placenta.
Involvement in diseaseDefects in LDB3 are the cause of cardiomyopathy dilated type 1C (CMD1C) [MIM:601493]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in LDB3 are the cause of left ventricular non-compaction type 3 (LVNC3) [MIM:601493]. Left ventricular non-compaction is characterized by numerous prominent trabeculations and deep intertrabecular recesses in hypertrophied and hypokinetic segments of the left ventricle.
Defects in LDB3 are the cause of myopathy myofibrillar ZASP-related (MFM-ZASP) [MIM:609452]. A neuromuscular disorder characterized by distal and proximal muscle weakness with signs of cardiomyopathy and neuropathy.
Sequence similaritiesContains 3 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain.
Cellular localizationCytoplasm > perinuclear region. Cell projection > pseudopodium. Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Localized to the cytoplasm around nuclei and pseudopodia of undifferentiated cells and detected throughout the myotubes of differentiated cells. Colocalizes with ACTN2 at the Z-lines.
- Information by UniProt
- CMD1C antibody
- CYPHER antibody
- HGNC:15710 antibody
All lanes : Anti-LDB3 antibody [EPR10126] (ab171936) at 1/1000 dilution
Lane 1 : A431 cell line lysate
Lane 2 : HT 1080 cell line lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat ant-rabbit HRP conjugated antibody at 1/500 dilution
Developed using the ECL technique.
Predicted band size: 77 kDa
ab171936 has not yet been referenced specifically in any publications.