Overview

  • Product name
    Anti-LDB3 antibody [EPR10127]
    See all LDB3 primary antibodies
  • Description
    Rabbit monoclonal [EPR10127] to LDB3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to amino acids of Human LDB3 (Uniprot O75112).

  • Positive control
    • Human skeletal muscle, rat muscle and mouse muscle lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10127
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 77 kDa.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      May function as an adapter in striated muscle to couple protein kinase C-mediated signaling via its LIM domains to the cytoskeleton.
    • Tissue specificity
      Expressed primarily in skeletal muscle and to a lesser extent in heart. Also detected in brain and placenta.
    • Involvement in disease
      Defects in LDB3 are the cause of cardiomyopathy dilated type 1C (CMD1C) [MIM:601493]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
      Defects in LDB3 are the cause of left ventricular non-compaction type 3 (LVNC3) [MIM:601493]. Left ventricular non-compaction is characterized by numerous prominent trabeculations and deep intertrabecular recesses in hypertrophied and hypokinetic segments of the left ventricle.
      Defects in LDB3 are the cause of myopathy myofibrillar ZASP-related (MFM-ZASP) [MIM:609452]. A neuromuscular disorder characterized by distal and proximal muscle weakness with signs of cardiomyopathy and neuropathy.
    • Sequence similarities
      Contains 3 LIM zinc-binding domains.
      Contains 1 PDZ (DHR) domain.
    • Cellular localization
      Cytoplasm > perinuclear region. Cell projection > pseudopodium. Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Localized to the cytoplasm around nuclei and pseudopodia of undifferentiated cells and detected throughout the myotubes of differentiated cells. Colocalizes with ACTN2 at the Z-lines.
    • Information by UniProt
    • Database links
    • Alternative names
      • CMD1C antibody
      • CYPHER antibody
      • HGNC:15710 antibody
      • KIAA01613 antibody
      • KIAA0613 antibody
      • Ldb3 antibody
      • LDB3_HUMAN antibody
      • LDB3Z1 antibody
      • LDB3Z4 antibody
      • LIM domain binding 3 antibody
      • LIM domain binding protein 3 antibody
      • LIM domain-binding protein 3 antibody
      • LVNC3 antibody
      • ORACLE antibody
      • PDLIM6 antibody
      • PDZ and LIM domain 6 antibody
      • Protein cypher antibody
      • Z band alternatively spliced PDZ motif antibody
      • Z band alternatively spliced PDZ motif protein antibody
      • Z-band alternatively spliced PDZ-motif protein antibody
      • ZASP antibody
      see all

    Images

    • All lanes : Anti-LDB3 antibody [EPR10127] (ab154183) at 1/10000 dilution

      Lane 1 : Human skeletal muscle lysates
      Lane 2 : Rat muscle lysates
      Lane 3 : Mouse muscle lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 77 kDa

    References

    This product has been referenced in:
    • Bakshi MV  et al. In-Utero Low-Dose Irradiation Leads to Persistent Alterations in the Mouse Heart Proteome. PLoS One 11:e0156952 (2016). WB ; Mouse . Read more (PubMed: 27276052) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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