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Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) ab102526 quantifies LDH activity in a variety of samples such as serum or plasma, cells, and culture medium.
In this assay LDH reduces NAD to NADH, which then interacts with a specific probe to produce a color (OD max = 450 nm). The kit can detect 1 - 100 mU/mL of LDH directly in samples. The assay is quick, convenient, and sensitive.
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As LDH is a fairly stable enzyme, LDH has been widely used to evaluate the presence of damage and toxicity of tissue and cells. Quantification of LDH has broad range of applications. The kit can detect 1-100 mU/ml of LDH directly in samples.
|LDH Assay Buffer||NM||1 x 50ml|
|LDH Positive Control (lyophilized)||Red||1 vial|
|LDH Substrate Mix||Amber||1 vial|
|NADH Standard||Yellow||1 vial|
Kinetic profiles of approx 0.5 mU of a sample of pure LDH (Positive Control) and 2 μL frozen human serum using buffer as a background control.
Cellular necrosis was measured as LDH release after cisplatin treatment for 24 h and 48 h using ab102526. The release of LDH was apparently increased at 24 and 48 h after cisplatin treatment.
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