Overview

  • Product name
    LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric)
    See all Lactate Dehydrogenase kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type
    Enzyme activity (quantitative)
  • Sensitivity
    > 1 mU/ml
  • Range
    1 mU/ml - 100 mU/ml
  • Assay time
    0h 30m
  • Product overview

    Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) ab102526 quantifies LDH activity in a variety of samples such as serum or plasma, cells, and culture medium.


    In the LDH assay protocol, LDH reduces NAD to NADH, which then interacts with a specific probe to produce a color (OD max = 450 nm). The kit can detect 1 - 100 mU/mL of LDH directly in samples. The assay is quick, convenient, and sensitive.


    LDH assay protocol summary:
    - add samples and standards to wells
    - add reaction mix
    - analyze every 2-3 min for at least 30 min with microplate reader in kinetic mode at 37ºC

  • Notes

    As LDH is a fairly stable enzyme, the LDH assay is widely used as a marker for damage to cells and tissues, ie. to measure damage that is severe enough to allow LDH to leak from the cell.

  • Platform
    Microplate reader

Properties

Images

  • Cellular necrosis was measured as LDH release after cisplatin treatment for 24 h and 48 h using ab102526. The release of LDH was apparently increased at 24 and 48 h after cisplatin treatment.

  • Kinetic profiles of approx 0.5 mU of a sample of pure LDH (Positive Control) and 2 μL frozen human serum using buffer as a background control.

  • NADH Standard Curve

Protocols

References

This product has been referenced in:
  • Wang Q  et al. miR-223-3p/TIAL1 interaction is involved in the mechanisms associated with the neuroprotective effects of dexmedetomidine on hippocampal neuronal cells in vitro. Mol Med Rep 19:805-812 (2019). Read more (PubMed: 30569136) »
  • Zou X  et al. Renal scattered tubular-like cells confer protective effects in the stenotic murine kidney mediated by release of extracellular vesicles. Sci Rep 8:1263 (2018). Read more (PubMed: 29352176) »
See all 28 Publications for this product

Customer reviews and Q&As

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Abreview forLDH Assay Kit ( ab102526)

Good Good 4/5 (Ease of Use)
Abreviews
Set up 48-well plate using MM1.S cells.
Collect 60 ul of supernatant from each well and treat with different concentrations of PEG (600-3000)
At final timepoint, collect all cells, spin celle to pellet and add 100 ul of LDH lysis buffer.
Reaction:
50 ul of supernatant+50 ul of LDH substrate—RT,30 min
40 ul of PBS+10 ul of cell lysate+50 ul of LDH substrate—RT,30 min
Add 50 ul of stop solution.
Measuren absorbance at 490 nm

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Submitted Nov 02 2018

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