LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (ab102526)
- Datasheet
- References (28)
- Protocols
Overview
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Product nameLDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric)
See all Lactate Dehydrogenase kits -
Detection methodColorimetric
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Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
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Assay typeEnzyme activity (quantitative)
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Sensitivity> 1 mU/ml
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Range1 mU/ml - 100 mU/ml
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Assay time0h 30m
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Product overview
Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) ab102526 quantifies LDH activity in a variety of samples such as serum or plasma, cells, and culture medium.
In the LDH assay protocol, LDH reduces NAD to NADH, which then interacts with a specific probe to produce a color (OD max = 450 nm). The kit can detect 1 - 100 mU/mL of LDH directly in samples. The assay is quick, convenient, and sensitive.
LDH assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze every 2-3 min for at least 30 min with microplate reader in kinetic mode at 37ºC -
Notes
As LDH is a fairly stable enzyme, the LDH assay is widely used as a marker for damage to cells and tissues, ie. to measure damage that is severe enough to allow LDH to leak from the cell.
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PlatformMicroplate reader
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components Identifier 500 tests LDH Assay Buffer NM 1 x 50ml LDH Positive Control (lyophilized) Red 1 vial LDH Substrate Mix Amber 1 vial NADH Standard Yellow 1 vial -
Research areas
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PathwayFermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
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Involvement in diseaseDefects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
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Sequence similaritiesBelongs to the LDH/MDH superfamily. LDH family.
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Post-translational
modificationsISGylated. -
Cellular localizationCytoplasm.
- Information by UniProt
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Alternative names
- Cell proliferation-inducing gene 19 protein
- GSD11
- L lactate dehydrogenase B chain
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Associated products
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Assay kits
Images
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Functional Studies - Lactate Dehydrogenase Colorimetric Assay Kit (ab102526)Image from Xu Y et al., PLoS One. 2014;9(6):e100127. Fig 2(B).; doi: 10.1371/journal.pone.0100127.Cellular necrosis was measured as LDH release after cisplatin treatment for 24 h and 48 h using ab102526. The release of LDH was apparently increased at 24 and 48 h after cisplatin treatment.
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Functional Studies - Lactate Dehydrogenase Colorimetric Assay Kit (ab102526)Kinetic profiles of approx 0.5 mU of a sample of pure LDH (Positive Control) and 2 μL frozen human serum using buffer as a background control.
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Functional Studies - Lactate Dehydrogenase Colorimetric Assay Kit (ab102526)NADH Standard Curve
Protocols
References
This product has been referenced in:
- Wang Q et al. miR-223-3p/TIAL1 interaction is involved in the mechanisms associated with the neuroprotective effects of dexmedetomidine on hippocampal neuronal cells in vitro. Mol Med Rep 19:805-812 (2019). Read more (PubMed: 30569136) »
- Zou X et al. Renal scattered tubular-like cells confer protective effects in the stenotic murine kidney mediated by release of extracellular vesicles. Sci Rep 8:1263 (2018). Read more (PubMed: 29352176) »
