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LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (ab102526)

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  • SDS
  • Protocol Booklet
Reviews (1)Q&A (19)References (182)

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LDH assay on mouse skeletal muscle
  • Functional Studies - Lactate Dehydrogenase Colorimetric Assay Kit (ab102526)
  • Functional Studies - Lactate Dehydrogenase Colorimetric Assay Kit (ab102526)
  • Functional Studies - Lactate Dehydrogenase Colorimetric Assay Kit (ab102526)

Key features and details

  • Assay type: Enzyme activity (quantitative)
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Assay time: 30 min
  • Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
  • Sensitivity: 1 mU/ml

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Overview

  • Product name

    LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric)
    See all Lactate Dehydrogenase kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type

    Enzyme activity (quantitative)
  • Sensitivity

    > 1 mU/ml
  • Range

    1 mU/ml - 100 mU/ml
  • Assay time

    0h 30m
  • Product overview

    Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) ab102526 quantifies LDH activity in a variety of samples such as serum or plasma, tissue, cells, and culture medium.


    In the LDH assay protocol, LDH reduces NAD to NADH, which then interacts with a specific probe to produce a color (OD max = 450 nm). The kit can detect 1 - 100 mU/mL of LDH directly in samples. The assay is quick, convenient, and sensitive.


    LDH assay protocol summary:
    - add samples and standards to wells
    - add reaction mix
    - analyze every 2-3 min for at least 30 min with microplate reader in kinetic mode at 37ºC

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K726 Lactate Dehydrogenase Activity Colorimetric Assay Kit. K726-500 is the same size as the 500 test size of ab102526.

    To measure LDH release into cell culture medium from cultured cells in a cytoxicity experiment, we recommend LDH assay kit ab65393, which is designed specifically as a cytotoxicity assay.

    This LDH assay kit ab102526 is designed in a more flexible format for use with a variety of sample types (this includes cell culture medium, and this kit may be used in cytotoxicity assays). This flexible format LDH assay kit is also available as a fluorometric LDH assay kit ab197000.

     

    How other researchers have used LDH Assay Kit ab102526

    This LDH assay kit has been used in publications in a variety of sample types, including:
    - Human: serum1
    - Mouse: kidney tissue2, kidney epithelial cell line lysates3, muscle tissue4, serum2,5, vaginal lavage fluid6, liver tissue7
    - Rat: H8C2 cell lysates8

    It has also been used in LDH release cytotoxicity cell culture assays including in human hippocampal neuronal cultures9, THP-1 cells10, primary chondrocyte cells on a membrane scaffold11, primary lymphocytes12, ovarian cancer cells co-cultured with mouse splenocytes in a cytotoxic T lymphocyte assay13

    References: 1-Zhang Z et al 2018, Solivio MJ et al 2016; 2-Niles DJ et al 2018; 3-Tanner LB et al 2018; 4-Tam BT et al 2018; 5-Kim HY et al 2016; 6-Nash EE et al 2016; 7-Lai C et al 2018; 8-Zhou Z et al 2018; 9-Wang Q et al 2019; 10-Alimov I et al 2019; 11-Stocco TD et al 2019; 12-Dreisig K et al 2018; 13-Sun Y et al 2018

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 500 tests
    LDH Assay Buffer NM 1 x 50ml
    LDH Positive Control (lyophilized) Red 1 vial
    LDH Substrate Mix (lyophilized) Amber 1 vial
    NADH Standard (lyophilized) Yellow 1 vial
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • Lactic Dehydrogenase
    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Sugar Assays
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Kits/ Lysates/ Other
    • Kits
    • Cell Damage Kits
    • Cell viability, plasma membrane damage
    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Lipid Metabolism Kits
    • Insulin signaling
    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Lipid Metabolism Kits
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Kits/ Lysates/ Other
    • Kits
    • Cell Damage Kits
    • Cell Damage
  • Pathway

    Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
  • Involvement in disease

    Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
  • Sequence similarities

    Belongs to the LDH/MDH superfamily. LDH family.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P00338 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Cell proliferation-inducing gene 19 protein
    • GSD11
    • L lactate dehydrogenase B chain
    • L-lactate dehydrogenase A chain
    • Lactate dehydrogenase A
    • Lactate dehydrogenase B
    • Lactate dehydrogenase c variant 1
    • Lactate dehydrogenase c variant 3
    • Lactate dehydrogenase c variant 4
    • Lactate dehydrogenase C4
    • Lactate dehydrogenase H chain
    • Lactate dehydrogenase M
    • LDH A
    • LDH B
    • LDH H
    • LDH heart subunit
    • LDH M
    • LDH muscle subunit
    • LDH-A
    • LDH-M
    • LDH1
    • ldha
    • LDHA_HUMAN
    • LDHBD
    • LDHM
    • MS1111
    • PIG19
    • Proliferation inducing gene 19
    • Proliferation-inducing gene 19
    • Renal carcinoma antigen NY REN 46
    • Renal carcinoma antigen NY-REN-59
    • TRG 5
    • TRG5
    see all

Associated products

  • Assay kits

    • Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (ab197000)
    • LDH-Cytotoxicity Assay Kit (Fluorometric) (ab197004)
    • LDH Assay Kit (Cytotoxicity) (ab65393)

Images

  • LDH assay on mouse skeletal muscle
    LDH assay on mouse skeletal muscleTam BT et al. 2018 Sci Rep 8:3689 Use licensed under: http://creativecommons.org/licenses/by/4.0/

    Tam BT et al. used the LDH assay kit on mouse skeletal muscle tissue lysates comparing pressure-induced compressed and control samples across a range of knockout mice lines.

  • Functional Studies - Lactate Dehydrogenase Colorimetric Assay Kit (ab102526)
    Functional Studies - Lactate Dehydrogenase Colorimetric Assay Kit (ab102526)

    Kinetic profiles of approx 0.5 mU of a sample of pure LDH (Positive Control) and 2 μL frozen human serum using buffer as a background control.

  • Functional Studies - Lactate Dehydrogenase Colorimetric Assay Kit (ab102526)
    Functional Studies - Lactate Dehydrogenase Colorimetric Assay Kit (ab102526)

    NADH Standard Curve.

  • Functional Studies - Lactate Dehydrogenase Colorimetric Assay Kit (ab102526)
    Functional Studies - Lactate Dehydrogenase Colorimetric Assay Kit (ab102526)Image from Xu Y et al., PLoS One. 2014;9(6):e100127. Fig 2(B).; doi: 10.1371/journal.pone.0100127. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Cellular necrosis was measured as LDH release after cisplatin treatment for 24 h and 48 h using ab102526. The release of LDH was apparently increased at 24 and 48 h after cisplatin treatment.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (182)

Publishing research using ab102526? Please let us know so that we can cite the reference in this datasheet.

ab102526 has been referenced in 182 publications.

  • Liu K  et al. Trypsin-Mediated Sensitization to Ferroptosis Increases the Severity of Pancreatitis in Mice. Cell Mol Gastroenterol Hepatol 13:483-500 (2022). PubMed: 34562639
  • Feng Y  et al. Pyruvate kinase M2 (PKM2) improve symptoms of post-ischemic stroke depression by activating VEGF to mediate the MAPK/ERK pathway. Brain Behav 12:e2450 (2022). PubMed: 34898024
  • Yu Y  et al. MicroRNA-665-3p exacerbates nonalcoholic fatty liver disease in mice. Bioengineered 13:2927-2942 (2022). PubMed: 35038955
  • Alsemeh AE & Abdullah DM Protective effect of alogliptin against cyclophosphamide-induced lung toxicity in rats: Impact on PI3K/Akt/FoxO1 pathway and downstream inflammatory cascades. Cell Tissue Res 388:417-438 (2022). PubMed: 35107620
  • Cao X  et al. Mechanism of lncRNA SNHG16 in oxidative stress and inflammation in oxygen-glucose deprivation and reoxygenation-induced SK-N-SH cells. Bioengineered 13:5021-5034 (2022). PubMed: 35170375
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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Abreview forLDH Assay Kit ( ab102526)

Good Good 4/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Set up 48-well plate using MM1.S cells.
Collect 60 ul of supernatant from each well and treat with different concentrations of PEG (600-3000)
At final timepoint, collect all cells, spin celle to pellet and add 100 ul of LDH lysis buffer.
Reaction:
50 ul of supernatant+50 ul of LDH substrate—RT,30 min
40 ul of PBS+10 ul of cell lysate+50 ul of LDH substrate—RT,30 min
Add 50 ul of stop solution.
Measuren absorbance at 490 nm
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Nov 02 2018

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