Collect 60 ul of supernatant from each well and treat with different concentrations of PEG (600-3000)
At final timepoint, collect all cells, spin celle to pellet and add 100 ul of LDH lysis buffer.
50 ul of supernatant+50 ul of LDH substrate—RT,30 min
40 ul of PBS+10 ul of cell lysate+50 ul of LDH substrate—RT,30 min
Add 50 ul of stop solution.
Measuren absorbance at 490 nm
Abcam user community
Submitted Nov 02 2018