Anti-LDH-C antibody (ab222910)

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Overview

  • Product name

    Anti-LDH-C antibody
    See all LDH-C primary antibodies

  • Description

    Rabbit polyclonal to LDH-C

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig

  • Immunogen

    Recombinant full length protein corresponding to Human LDH-C aa 2-332.
    Sequence:

    STVKEQLIEKLIEDDENSQCKITIVGTGAVGMACAISILLKDLADELALV DVALDKLKGEMMDLQHGSLFFSTSKITSGKDYSVSANSRIVIVTAGARQQ EGETRLALVQRNVAIMKSIIPAIVHYSPDCKILVVSNPVDILTYIVWKIS GLPVTRVIGSGCNLDSARFRYLIGEKLGVHPTSCHGWIIGEHGDSSVPLW SGVNVAGVALKTLDPKLGTDSDKEHWKNIHKQVIQSAYEIIKLKGYTSWA IGLSVMDLVGSILKNLRRVHPVSTMVKGLYGIKEELFLSIPCVLGRNGVS DVVKINLNSEEEALFKKSAETLWNIQKDLIF


    Database link: P07864

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: NIH/3T3 and HepG2 whole cell lysates; Mouse brain, heart, lung and thymus tissue lysates; Rat liver tissue lysate. IHC-P: Human small intestine and testis tissues. ICC/IF: HeLa cells.

  • General notes

    Previously labelled as Lactate Dehydrogenase C. 

Properties

Applications

Our Abpromise guarantee covers the use of ab222910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
WB 1/500 - 1/5000. Predicted molecular weight: 37 kDa.
IHC-P 1/20 - 1/200.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDH-C antibody (ab222910)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDH-C antibody (ab222910)

    Paraffin-embedded human small intestine tissue stained for Lactate Dehydrogenase C / LDH-C using ab222910 at 1/100 dilution in immunohistochemical analysis.

  • Western blot - Anti-LDH-C antibody (ab222910)
    Western blot - Anti-LDH-C antibody (ab222910)
    All lanes : Anti-LDH-C antibody (ab222910) at 1/500 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : Mouse heart tissue lysate
    Lane 5 : Mouse lung tissue lysate
    Lane 6 : Mouse thymus tissue lysate
    Lane 7 : Rat liver tissue lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG

    Predicted band size: 37 kDa
    Observed band size: 37 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-LDH-C antibody (ab222910)
    Immunocytochemistry/ Immunofluorescence - Anti-LDH-C antibody (ab222910)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Lactate Dehydrogenase C / LDH-C (green) using ab222910 at 1/100 dilution in ICC/IF. Secondary: Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDH-C antibody (ab222910)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDH-C antibody (ab222910)

    Paraffin-embedded human testis tissue stained for Lactate Dehydrogenase C / LDH-C using ab222910 at 1/100 dilution in immunohistochemical analysis.

References

This product has been referenced in:

  • Liu X  et al. Aberrant expression of sperm-specific glycolytic enzymes are associated with poor sperm quality. Mol Med Rep 19:2471-2478 (2019). Read more (PubMed: 30720094) »
See 1 Publication for this product

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