Anti-LDH-C antibody (ab222910)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDH-C antibody (ab222910)
  • Western blot - Anti-LDH-C antibody (ab222910)
  • Immunocytochemistry/ Immunofluorescence - Anti-LDH-C antibody (ab222910)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDH-C antibody (ab222910)

Key features and details

  • Rabbit polyclonal to LDH-C
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-LDH-C antibody
    See all LDH-C primary antibodies

  • Description

    Rabbit polyclonal to LDH-C

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human LDH-C aa 1 to the C-terminus.
    Database link: P07864

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: NIH/3T3 and HepG2 whole cell lysates; Mouse brain, heart, lung and thymus tissue lysates; Rat liver tissue lysate. IHC-P: Human small intestine and testis tissues. ICC/IF: HeLa cells.

  • General notes

    Previously labelled as Lactate Dehydrogenase C. 

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab222910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Mouse
All applications
Pig
Application Abreviews Notes
ICC/IF
1/50 - 1/200.
WB
1/500 - 1/5000. Predicted molecular weight: 37 kDa.
IHC-P
1/20 - 1/200.
Notes
ICC/IF
1/50 - 1/200.
WB
1/500 - 1/5000. Predicted molecular weight: 37 kDa.
IHC-P
1/20 - 1/200.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDH-C antibody (ab222910)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDH-C antibody (ab222910)

    Paraffin-embedded human small intestine tissue stained for Lactate Dehydrogenase C / LDH-C using ab222910 at 1/100 dilution in immunohistochemical analysis.

  • Western blot - Anti-LDH-C antibody (ab222910)
    Western blot - Anti-LDH-C antibody (ab222910)
    All lanes : Anti-LDH-C antibody (ab222910) at 1/500 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : Mouse heart tissue lysate
    Lane 5 : Mouse lung tissue lysate
    Lane 6 : Mouse thymus tissue lysate
    Lane 7 : Rat liver tissue lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG

    Predicted band size: 37 kDa
    Observed band size: 37 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-LDH-C antibody (ab222910)
    Immunocytochemistry/ Immunofluorescence - Anti-LDH-C antibody (ab222910)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Lactate Dehydrogenase C / LDH-C (green) using ab222910 at 1/100 dilution in ICC/IF. Secondary: Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDH-C antibody (ab222910)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDH-C antibody (ab222910)

    Paraffin-embedded human testis tissue stained for Lactate Dehydrogenase C / LDH-C using ab222910 at 1/100 dilution in immunohistochemical analysis.

References (1)

Publishing research using ab222910? Please let us know so that we can cite the reference in this datasheet.

ab222910 has been referenced in 1 publication.

  • Liu X  et al. Aberrant expression of sperm-specific glycolytic enzymes are associated with poor sperm quality. Mol Med Rep 19:2471-2478 (2019). PubMed: 30720094

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