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    ldh-c-antibody-ab222910.pdf

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Signal Transduction Metabolism Energy Metabolism
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Anti-LDH-C antibody (ab222910)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDH-C antibody (ab222910)
  • Western blot - Anti-LDH-C antibody (ab222910)
  • Immunocytochemistry/ Immunofluorescence - Anti-LDH-C antibody (ab222910)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDH-C antibody (ab222910)

Key features and details

  • Rabbit polyclonal to LDH-C
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human LDH-C protein (ab158814)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-LDH-C antibody
    See all LDH-C primary antibodies
  • Description

    Rabbit polyclonal to LDH-C
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Recombinant full length protein corresponding to Human LDH-C aa 1 to the C-terminus.
    Database link: P07864

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: NIH/3T3 and HepG2 whole cell lysates; Mouse brain, heart, lung and thymus tissue lysates; Rat liver tissue lysate. IHC-P: Human small intestine and testis tissues. ICC/IF: HeLa cells.
  • General notes

    Previously labelled as Lactate Dehydrogenase C. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • Lactic Dehydrogenase
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Hep G2 whole cell lysate (ab166833)
    • Mouse thymus tissue lysate - total protein (ab29285)
    • Mouse lung normal tissue lysate - total protein (ab29297)
    • Mouse heart normal tissue lysate - total protein (ab30291)
    • Mouse brain tissue lysate - total protein (ab4022)
    • NIH/3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant Human LDH-C protein (ab158814)
  • Related Products

    • Recombinant Human LDH-C protein (ab158814)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab222910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Mouse
All applications
Pig
Application Abreviews Notes
ICC/IF
1/50 - 1/200.
WB
1/500 - 1/5000. Predicted molecular weight: 37 kDa.
IHC-P
1/20 - 1/200.
Notes
ICC/IF
1/50 - 1/200.
WB
1/500 - 1/5000. Predicted molecular weight: 37 kDa.
IHC-P
1/20 - 1/200.

Target

  • Function

    Possible role in sperm motility.
  • Pathway

    Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
  • Sequence similarities

    Belongs to the LDH/MDH superfamily. LDH family.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P07864 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3948 Human
    • Entrez Gene: 16833 Mouse
    • Entrez Gene: 100502559 Pig
    • Entrez Gene: 29634 Rat
    • Omim: 150150 Human
    • SwissProt: P07864 Human
    • SwissProt: P00342 Mouse
    • SwissProt: Q9TSX5 Pig
    • SwissProt: P19629 Rat
    • Unigene: 654377 Human
    • Unigene: 16563 Mouse
    • Unigene: 9984 Rat
    see all
  • Alternative names

    • Cancer/testis antigen 32 antibody
    • CT32 antibody
    • EC 1.1.1.27 antibody
    • L lactate dehydrogenase C chain antibody
    • L-lactate dehydrogenase C chain antibody
    • Lactate Dehydrogenase C antibody
    • Lactate dehydrogenase c variant 1 antibody
    • Lactate dehydrogenase c variant 3 antibody
    • Lactate dehydrogenase c variant 4 antibody
    • Lactate dehydrogenase C4 antibody
    • LDH C antibody
    • LDH testis subunit antibody
    • LDH X antibody
    • LDH-C antibody
    • LDH-X antibody
    • LDH3 antibody
    • ldhc antibody
    • LDHC_HUMAN antibody
    • LDHX antibody
    • MGC111073 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDH-C antibody (ab222910)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDH-C antibody (ab222910)

    Paraffin-embedded human small intestine tissue stained for Lactate Dehydrogenase C / LDH-C using ab222910 at 1/100 dilution in immunohistochemical analysis.

  • Western blot - Anti-LDH-C antibody (ab222910)
    Western blot - Anti-LDH-C antibody (ab222910)
    All lanes : Anti-LDH-C antibody (ab222910) at 1/500 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : Mouse heart tissue lysate
    Lane 5 : Mouse lung tissue lysate
    Lane 6 : Mouse thymus tissue lysate
    Lane 7 : Rat liver tissue lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG

    Predicted band size: 37 kDa
    Observed band size: 37 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-LDH-C antibody (ab222910)
    Immunocytochemistry/ Immunofluorescence - Anti-LDH-C antibody (ab222910)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Lactate Dehydrogenase C / LDH-C (green) using ab222910 at 1/100 dilution in ICC/IF. Secondary: Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDH-C antibody (ab222910)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDH-C antibody (ab222910)

    Paraffin-embedded human testis tissue stained for Lactate Dehydrogenase C / LDH-C using ab222910 at 1/100 dilution in immunohistochemical analysis.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab222910? Please let us know so that we can cite the reference in this datasheet.

    ab222910 has been referenced in 1 publication.

    • Liu X  et al. Aberrant expression of sperm-specific glycolytic enzymes are associated with poor sperm quality. Mol Med Rep 19:2471-2478 (2019). PubMed: 30720094

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab222910.
    Please use the links above to contact us or submit feedback about this product.

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