Anti-LDH-C antibody (ab222910)
Key features and details
- Rabbit polyclonal to LDH-C
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-LDH-C antibody
See all LDH-C primary antibodies -
Description
Rabbit polyclonal to LDH-C -
Host species
Rabbit -
Tested Applications & Species
Application Species ICC/IF HumanIHC-P HumanWB Mouse -
Immunogen
Recombinant full length protein corresponding to Human LDH-C aa 1 to the C-terminus.
Database link: P07864 -
Positive control
- WB: NIH/3T3 and HepG2 whole cell lysates; Mouse brain, heart, lung and thymus tissue lysates; Rat liver tissue lysate. IHC-P: Human small intestine and testis tissues. ICC/IF: HeLa cells.
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General notes
Previously labelled as Lactate Dehydrogenase C.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300 -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab222910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
ICC/IF |
Human
|
IHC-P |
Human
|
WB |
Mouse
|
All applications |
Pig
|
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/50 - 1/200.
|
|
WB |
1/500 - 1/5000. Predicted molecular weight: 37 kDa.
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|
IHC-P |
1/20 - 1/200.
|
Notes |
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ICC/IF
1/50 - 1/200. |
WB
1/500 - 1/5000. Predicted molecular weight: 37 kDa. |
IHC-P
1/20 - 1/200. |
Target
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Function
Possible role in sperm motility. -
Pathway
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. -
Sequence similarities
Belongs to the LDH/MDH superfamily. LDH family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 3948 Human
- Entrez Gene: 16833 Mouse
- Entrez Gene: 100502559 Pig
- Entrez Gene: 29634 Rat
- Omim: 150150 Human
- SwissProt: P07864 Human
- SwissProt: P00342 Mouse
- SwissProt: Q9TSX5 Pig
see all -
Alternative names
- Cancer/testis antigen 32 antibody
- CT32 antibody
- EC 1.1.1.27 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDH-C antibody (ab222910)
Paraffin-embedded human small intestine tissue stained for Lactate Dehydrogenase C / LDH-C using ab222910 at 1/100 dilution in immunohistochemical analysis.
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All lanes : Anti-LDH-C antibody (ab222910) at 1/500 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : Mouse heart tissue lysate
Lane 5 : Mouse lung tissue lysate
Lane 6 : Mouse thymus tissue lysate
Lane 7 : Rat liver tissue lysate
Secondary
All lanes : Goat Anti-Rabbit IgG
Predicted band size: 37 kDa
Observed band size: 37 kDa -
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Lactate Dehydrogenase C / LDH-C (green) using ab222910 at 1/100 dilution in ICC/IF. Secondary: Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDH-C antibody (ab222910)
Paraffin-embedded human testis tissue stained for Lactate Dehydrogenase C / LDH-C using ab222910 at 1/100 dilution in immunohistochemical analysis.
Protocols
References (1)
ab222910 has been referenced in 1 publication.
- Liu X et al. Aberrant expression of sperm-specific glycolytic enzymes are associated with poor sperm quality. Mol Med Rep 19:2471-2478 (2019). PubMed: 30720094