• Product name
    Anti-LDH-C antibody [EP1746Y]
    See all LDH-C primary antibodies
  • Description
    Rabbit monoclonal [EP1746Y] to LDH-C
  • Host species
  • Specificity
    This antibody reacts with Lactate Dehydrogenase C / LDH-C. The immunogen sequence is 100% identical to Lactate Dehydrogenase C / LDH-C, but there is the possibility of cross-reactivity for lactate dehydrogenase A and lactate dehydrogenase B at 69% and 82%, respectively.
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-Pmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human LDH-C aa 300 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P07864

  • Positive control
    • WB: Human testis lysate. IHC-P: Human testis carcinoma tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Previously labelled as Lactate Dehydrogenase C. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.



Our Abpromise guarantee covers the use of ab52747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/20000. Detects a band of approximately 31 kDa (predicted molecular weight: 36 kDa).
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Function
      Possible role in sperm motility.
    • Pathway
      Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
    • Sequence similarities
      Belongs to the LDH/MDH superfamily. LDH family.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • Cancer/testis antigen 32 antibody
      • CT32 antibody
      • EC antibody
      • L lactate dehydrogenase C chain antibody
      • L-lactate dehydrogenase C chain antibody
      • Lactate Dehydrogenase C antibody
      • Lactate dehydrogenase c variant 1 antibody
      • Lactate dehydrogenase c variant 3 antibody
      • Lactate dehydrogenase c variant 4 antibody
      • Lactate dehydrogenase C4 antibody
      • LDH C antibody
      • LDH testis subunit antibody
      • LDH X antibody
      • LDH-C antibody
      • LDH-X antibody
      • LDH3 antibody
      • ldhc antibody
      • LDHC_HUMAN antibody
      • LDHX antibody
      • MGC111073 antibody
      see all


    • Anti-LDH-C antibody [EP1746Y] (ab52747) at 1/20000 dilution + Human testis lysate at 10 µg

      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size: 36 kDa
      Observed band size: 31 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemical staining of paraffin-embedded human testis carcinoma using ab52747 at a 1/50 dilution.


    This product has been referenced in:
    • Muranaka F  et al. A Comparative Immunohistochemical Study of Anal Canal Epithelium in Humans and Swine, Focusing on the Anal Transitional Zone Epithelium and the Anal Glands. Anat Rec (Hoboken) N/A:N/A (2017). Read more (PubMed: 29232767) »
    • Rolland AD  et al. Identification of genital tract markers in the human seminal plasma using an integrative genomics approach. Hum Reprod 28:199-209 (2013). Read more (PubMed: 23024119) »
    See all 3 Publications for this product

    Customer reviews and Q&As


    Thank you for contacting us.
    I'm sorry to hear that this antibody is not working as expected.

    As we discussed over the phone, could you please let me know the following protocol information so that I can assist in troubleshooting or determine where the problem might lie:

    1) What antigen retrieval step was used?
    2) What was used for blocking?
    3) What was used as detection system and how is it working with other antibodies?
    4) What is your order or PO number?

    I look forward to hear back from you and help you further.

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