Product nameAnti-LDH-C antibody [EP1746Y]
See all LDH-C primary antibodies
DescriptionRabbit monoclonal [EP1746Y] to LDH-C
SpecificityThis antibody reacts with Lactate Dehydrogenase C / LDH-C. The immunogen sequence is 100% identical to Lactate Dehydrogenase C / LDH-C, but there is the possibility of cross-reactivity for lactate dehydrogenase A and lactate dehydrogenase B at 69% and 82%, respectively.
Tested applicationsSuitable for: WB, Flow Cyt, IHC-Pmore details
Unsuitable for: ICC or IP
Species reactivityReacts with: Human
Synthetic peptide within Human LDH-C aa 300 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P07864
- WB: Human testis lysate. IHC-P: Human testis carcinoma tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as Lactate Dehydrogenase C.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab52747 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/20000. Detects a band of approximately 31 kDa (predicted molecular weight: 36 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||Use at an assay dependent concentration.|
FunctionPossible role in sperm motility.
PathwayFermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Sequence similaritiesBelongs to the LDH/MDH superfamily. LDH family.
- Information by UniProt
- Cancer/testis antigen 32 antibody
- CT32 antibody
- EC 126.96.36.199 antibody
Anti-LDH-C antibody [EP1746Y] (ab52747) at 1/20000 dilution + Human testis lysate at 10 µg
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 36 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted?
Immunohistochemical staining of paraffin-embedded human testis carcinoma using ab52747 at a 1/50 dilution.
This product has been referenced in:
- Muranaka F et al. A Comparative Immunohistochemical Study of Anal Canal Epithelium in Humans and Swine, Focusing on the Anal Transitional Zone Epithelium and the Anal Glands. Anat Rec (Hoboken) N/A:N/A (2017). Read more (PubMed: 29232767) »
- Rolland AD et al. Identification of genital tract markers in the human seminal plasma using an integrative genomics approach. Hum Reprod 28:199-209 (2013). Read more (PubMed: 23024119) »