• Product name
    LDH-Cytotoxicity Assay Kit (400 assays)
    See all Lactate dehydrogenase kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Enzyme activity
  • Assay time
    1h 00m
  • Product overview

    LDH-Cytotoxicity Assay Kit (ab65391) provides a fast and simple method for quantitating cytotoxicity based on the measurement of activity of lactate dehydrogenase (LDH) released from damaged cells. The LDH-cytotoxicity assay is sensitive, convenient, and precise, and is applicable to a variety of cytotoxicity studies. Assay takes ~0.5-1 hr. LDH activity can be determined by a coupled enzymatic reaction: LDH oxidizes lactate to pyruvate which then reacts with tetrazolium salt INT to form formazan. The increase in the amount of formazan produced in culture supernatant directly correlates to the increase in the number of lysed cells. The formazan dye is water-soluble and can be detected by spectrophotometer at 500 nm.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. Unlike many other cytoplasmic enzymes which exist in many cells either in low amount (e.g., alkaline and acid phosphatase) or unstable, LDH is a stable cytoplasmic enzyme present in all cells and rapidly released into the cell culture supernatant upon damage of the plasma membrane.

    If you would like to use a fluorometric reading, please refer to LDH-Cytotoxicity Assay Kit (Fluorometric) (ab197004).


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 400 tests
    Catalyst (Lyophilized) 1 vial
    Dye Solution 1 x 45ml
  • Research areas
  • Relevance
    Lactate dehydrogenase (LDH) is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream).
  • Cellular localization
  • Alternative names
    • Cell proliferation inducing gene 19 protein
    • Epididymis secretory protein Li 281
    • Epididymis secretory sperm binding protein Li 133P
    • GSD11
    • HEL S 133P
    • HEL S 281
    • L lactate dehydrogenase A chain
    • L lactate dehydrogenase B chain
    • Lactate dehydrogenase A
    • Lactate dehydrogenase B
    • Lactate dehydrogenase H chain
    • Lactate dehydrogenase M
    • LDH H
    • LDH heart subunit
    • LDH1
    • LDHA
    • LDHB
    • LDHBD
    • LDHM
    • PIG19
    • Proliferation inducing gene 19
    • Renal carcinoma antigen NY REN 46
    • Renal carcinoma antigen NY REN 59
    • TRG 5
    see all


  • LDH release measured at various cell amounts


  • Jurkat cells were cultured in a 96-well plate in 100 µl of culture medium. LDH assay was performed using 10 µl of culture medium according to the kit instructions. Light bar: Low control; Dark bar: High control.



This product has been referenced in:
  • Gouriou Y  et al. Mitochondrial Ca2+ uptake from plasma membrane Cav3.2 protein channels contributes to ischemic toxicity in PC12 cells. J Biol Chem 288:12459-68 (2013). Read more (PubMed: 23508951) »
  • Spampanato C  et al. Transcription factor EB (TFEB) is a new therapeutic target for Pompe disease. EMBO Mol Med 5:691-706 (2013). Read more (PubMed: 23606558) »

See all 2 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

I have heard back from the lab with the following information:

There are numerous differences between these two assay kits. For details, I have attached the datasheets, but here are the main differences: Read More

Thank you for your reply, and I'm sorry that we could not solve this problem for you.

I'm happy to issue the refund. Do you have your order number, PO number, or date of the purchase?

Please let me know if you have any questions or ...

Read More

Thank you for your call this week and for your patience while I have been in touch with the lab.

The scientists at the lab have confirmed that the absorbance value should increase with increasing cytotoxicity, so the data is contradicting wha...

Read More

Thank you for contacting us and your interest in our products.

The LDH-Cytotoxicity Assay Kit (ab65391) is designed to measure the levels of LDH in the cell culture supernatants and determines the cytotoxicitybased on this. The kit can be the...

Read More

Thank you for contacting us. This kit is compatible with mammalian samples and hence will work with mouse and rat samples. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Thank you for your inquiry. There are a few differences between those two kits: With the LDH-Cytotoxicity Assay Kit ab65391 LDH activity is determined by a coupled enzymatic reaction. This assay kit is sensitive, convenient, and precise, and is ...

Read More

Merci de nous avoir contactés. Mes collaborateurs m'ont informé que le kit ab102526 est le plus adapté à vos besoins. Le lien vers la fiche technique est : www.abcam.com/ab102526. J'espère que cette information vous est utile. N'hésitez pas à n...

Read More

As long as your plate reader has the ability to measure the absorbance of all samples at 490-500 nm, then you will be able to use the plate reader with the LDH-Cytotoxicity assay kit. I believe that the plate reader that you have, does have the abilit...

Read More

Thank you for your phone call today and for your questions about our LDH assay kits. The kits do utilize different reactions in order to determine DLH activity, but there are also some other differences. I have summarized the advantages of each kit ...

Read More


Sign up