LDH-Cytotoxicity Assay Kit (Fluorometric) (ab197004)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
LDH-Cytotoxicity Assay Kit (Fluorometric)
See all Lactate Dehydrogenase kits -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Enzyme activity (quantitative) -
Sensitivity
100 cells/well -
Assay time
0h 20m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
LDH-Cytotoxicity Assay Kit (Fluorometric) (ab197004) provides a sensitive, quick, and easy way to detect LDH (lactate dehydrogenase) released from damaged cells. In this assay, LDH converts lactate to pyruvate and NADH, which reduces a proprietary probe to an intensely fluorescent product (Ex/Em = 535/587 nm). The amount of fluorescence is directly proportional to the number of damaged cells. The assay is adaptable to high-throughput format and can be completed in less than 20 min. Sensitivity: ~ 100 cells.
Review our cell health assays guide to learn more about our other cell viability, cytotoxicity and cell proliferation assay kits.
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Notes
Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. Lactate dehydragenase (LDH, EC 1.1.1.27) is a stable enzyme, present in all cell types, which is rapidly released into the cell culture medium upon damage of the plasma membrane. LDH, therefore, is the most widely used marker in cytotoxicity study.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 500 tests Cell Lysis Solution 1 x 5ml LDH Assay Buffer 1 x 50ml LDH Substrate Mix Amber 1 vial PicoProbe Blue 1 x 2ml Positive Control 1 x 20µl -
Research areas
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Pathway
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. -
Involvement in disease
Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue. -
Sequence similarities
Belongs to the LDH/MDH superfamily. LDH family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- Cell proliferation-inducing gene 19 protein
- GSD11
- L lactate dehydrogenase B chain
see all -
Database links
- SwissProt: Q7SI97 Other species
Associated products
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Assay kits
Images
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Untreated Jurkat cells or treated with Cell Lysis Solution.Untreated Jurkat cells (low control) or treated with Cell Lysis Solution for 30 min (high control).
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Relative Fluorescence Units of untreated Jurkat cells or treated with Cell Lysis Solution.Relative fluorescence units of untreated Jurkat cells (low control) or treated with Cell Lysis Solution for 30 min (high control).
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Overnight treatment of HeLa cells with staurosporine or cycloheximide.Overnight treatment of HeLa cells with 100μM of staurosporine or 3μM of cycloheximide. LDH released into the medium was measured along with blank, untreated cells (low control), LDH Positive Control, and lysed cells (high control)
Protocols
References
This product has been referenced in:
- Sillman B et al. Creation of a long-acting nanoformulated dolutegravir. Nat Commun 9:443 (2018). Read more (PubMed: 29402886) »
