LDH-Cytotoxicity Assay Kit (Fluorometric) (ab197004)

Submit a review Submit a question References (4)
Untreated Jurkat cells or treated with Cell Lysis Solution.
  • Relative Fluorescence Units of untreated Jurkat cells or treated with Cell Lysis Solution.
  • Overnight treatment of HeLa cells with staurosporine or cycloheximide.

Key features and details

  • Assay type: Enzyme activity (quantitative)
  • Detection method: Fluorescent
  • Platform: Microplate reader
  • Assay time: 20 min
  • Sample type: Adherent cells, Suspension cells
  • Sensitivity: 100 cells/well

Overview

  • Product name

    LDH-Cytotoxicity Assay Kit (Fluorometric)
    See all Lactate Dehydrogenase kits

  • Detection method

    Fluorescent

  • Sample type

    Adherent cells, Suspension cells

  • Assay type

    Enzyme activity (quantitative)

  • Sensitivity

    100 cells/well

  • Assay time

    0h 20m

  • Species reactivity

    Reacts with: Mammals, Other species

  • Product overview

    LDH Assay Kit (Cytotoxicity, Fluorometric) (ab197004) provides a sensitive, quick, and easy way to detect LDH (lactate dehydrogenase) released from damaged cells.


    In the LDH assay, LDH converts lactate to pyruvate and NADH, which reduces a proprietary probe to an intensely fluorescent product (Ex/Em = 535/587 nm).


    The amount of fluorescence is directly proportional to the number of damaged cells.


    The assay is adaptable to high-throughput format and can be completed in less than 20 min. Sensitivity: ~ 100 cells. 5µl of cell culture media required per assay.

  • Notes

    Alternative LDH assays

    If you would like to use a fluorometric assay, please refer to LDH-Cytotoxicity Assay Kit (Fluorometric) (ab197004). 

    This kit is more sensitive than the colorimetric LDH Cytoxicity Assay Kit ab65391.

    To measure LDH activity in sample types such as serum, plasma, and cell lysates, we recommend LDH assay kit ab102526.

    Related products and guides to cytotoxicity / cell viability / proliferation assays

    Review our cell health assay guide to learn about our other kits to perform a cell viability assay, cytotoxicity assay or cell proliferation assay.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate reader

Properties

Images

  • Untreated Jurkat cells or treated with Cell Lysis Solution.
    Untreated Jurkat cells or treated with Cell Lysis Solution.

    Untreated Jurkat cells (low control) or treated with Cell Lysis Solution for 30 min (high control).

  • Relative Fluorescence Units of untreated Jurkat cells or treated with Cell Lysis Solution.
    Relative Fluorescence Units of untreated Jurkat cells or treated with Cell Lysis Solution.

    Relative fluorescence units of untreated Jurkat cells (low control) or treated with Cell Lysis Solution for 30 min (high control).

  • Overnight treatment of HeLa cells with staurosporine or cycloheximide.
    Overnight treatment of HeLa cells with staurosporine or cycloheximide.

    Overnight treatment of HeLa cells with 100μM of staurosporine or 3μM of cycloheximide. LDH released into the medium was measured along with blank, untreated cells (low control), LDH Positive Control, and lysed cells (high control)

References (4)

Publishing research using ab197004? Please let us know so that we can cite the reference in this datasheet.

ab197004 has been referenced in 4 publications.

  • Du S  et al. Does Maternal Stress Affect the Early Embryonic Microenvironment? Impact of Long-Term Cortisol Stimulation on the Oviduct Epithelium. Int J Mol Sci 21:N/A (2020). PubMed: 32284519
  • Sharma P  et al. Cadmium exposure activates Akt/ERK Signaling and pro-inflammatory COX-2 expression in human gallbladder epithelial cells via a ROS dependent mechanism. Toxicol In Vitro 67:104912 (2020). PubMed: 32512147
  • Oyama Y  et al. Intense Light-Mediated Circadian Cardioprotection via Transcriptional Reprogramming of the Endothelium. Cell Rep 28:1471-1484.e11 (2019). PubMed: 31390562
  • Sillman B  et al. Creation of a long-acting nanoformulated dolutegravir. Nat Commun 9:443 (2018). PubMed: 29402886

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab197004.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com