Overview

  • Product name
    LDH-Cytotoxicity Assay Kit (Fluorometric)
    See all Lactate Dehydrogenase kits
  • Detection method
    Fluorescent
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Enzyme activity (quantitative)
  • Sensitivity
    100 cells/well
  • Assay time
    0h 20m
  • Species reactivity
    Reacts with: Mammals, Other species
  • Product overview

    LDH-Cytotoxicity Assay Kit (Fluorometric) (ab197004) provides a sensitive, quick, and easy way to detect LDH (lactate dehydrogenase) released from damaged cells. In this assay, LDH converts lactate to pyruvate and NADH, which reduces a proprietary probe to an intensely fluorescent product (Ex/Em = 535/587 nm). The amount of fluorescence is directly proportional to the number of damaged cells. The assay is adaptable to high-throughput format and can be completed in less than 20 min. Sensitivity: ~ 100 cells.


    Review our cell health assays guide to learn more about our other cell viability, cytotoxicity and cell proliferation assay kits.

  • Notes

    Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. Lactate dehydragenase (LDH, EC 1.1.1.27) is a stable enzyme, present in all cell types, which is rapidly released into the cell culture medium upon damage of the plasma membrane. LDH, therefore, is the most widely used marker in cytotoxicity study.

  • Platform
    Microplate reader

Properties

Images

  • Untreated Jurkat cells (low control) or treated with Cell Lysis Solution for 30 min (high control).

  • Relative fluorescence units of untreated Jurkat cells (low control) or treated with Cell Lysis Solution for 30 min (high control).

  • Overnight treatment of HeLa cells with 100μM of staurosporine or 3μM of cycloheximide. LDH released into the medium was measured along with blank, untreated cells (low control), LDH Positive Control, and lysed cells (high control)

Protocols

References

This product has been referenced in:
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