Key features and details
- Rabbit polyclonal to LDHA
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LDHA antibody
See all LDHA primary antibodies
DescriptionRabbit polyclonal to LDHA
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 133-310 of Human LDHA. (Uniprot:P00338)
- A431 cell lysate, 293T cell lysate, Jurkat cell lysate, Raji cell lysate and Human Lung papillory tissue.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab125683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 37 kDa.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/500.|
PathwayFermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Involvement in diseaseDefects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
Sequence similaritiesBelongs to the LDH/MDH superfamily. LDH family.
- Information by UniProt
- Cell proliferation-inducing gene 19 protein antibody
- GSD11 antibody
- L lactate dehydrogenase A chain antibody
Anti-LDHA antibody (ab125683) at 1/1000 dilution + A431 lysate at 30 µg
Predicted band size: 37 kDa
ab125683, at 1/500 dilution, staining LDHA in Formalin-fixed, Paraffin-embedded Human Lung papillory tissue by Immunohistochemistry.
ab125683, at 1/500 dilution, staining LDHA in Methanol-fixed A431 cells by Immunocytochemistry/Immunofluorescence.
ab125683 has been referenced in 3 publications.
- Yu L et al. Silencing the Girdin gene enhances radio-sensitivity of hepatocellular carcinoma via suppression of glycolytic metabolism. J Exp Clin Cancer Res 36:110 (2017). PubMed: 28810896
- Flores A et al. Lactate dehydrogenase activity drives hair follicle stem cell activation. Nat Cell Biol 19:1017-1026 (2017). PubMed: 28812580
- Xu H et al. PRL-3 improves colorectal cancer cell proliferation and invasion through IL-8 mediated glycolysis metabolism. Int J Oncol 51:1271-1279 (2017). PubMed: 28791350