Overview

  • Product name

  • Description

    Rabbit polyclonal to LDHA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Human, Pig, Zebrafish, Spider monkey
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 91-140 (LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPQ CKLLIVSNPV) of Rat LDHA (NP_058721).

  • Positive control

    • Rat stomach tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab135366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa.

Target

  • Pathway

    Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
  • Involvement in disease

    Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
  • Sequence similarities

    Belongs to the LDH/MDH superfamily. LDH family.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cell proliferation-inducing gene 19 protein antibody
    • GSD11 antibody
    • L lactate dehydrogenase A chain antibody
    • L-lactate dehydrogenase A chain antibody
    • l7R2 antibody
    • Lactate dehydrogenase 1, A chain antibody
    • Lactate dehydrogenase A antibody
    • Lactate dehydrogenase A4 antibody
    • Lactate dehydrogenase M antibody
    • LDH A antibody
    • LDH M antibody
    • LDH muscle subunit antibody
    • LDH muscle subunit; M LDH antibody
    • LDH-A antibody
    • LDH-M antibody
    • LDH1 antibody
    • ldha antibody
    • LDHA_HUMAN antibody
    • LDHM antibody
    • OTTMUSP00000017774 antibody
    • PIG19 antibody
    • Proliferation-inducing gene 19 antibody
    • Renal carcinoma antigen NY-REN-59 antibody
    see all

Images

  • Anti-LDHA antibody (ab135366) at 1 µg/ml + Rat stomach tissue lysate at 10 µg

    Predicted band size: 36 kDa

References

This product has been referenced in:

  • Abraham MA  et al. Leptin enhances hypothalamic lactate dehydrogenase A (LDHA)-dependent glucose sensing to lower glucose production in high-fat-fed rats. J Biol Chem 293:4159-4166 (2018). Read more (PubMed: 29374061) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Zebrafish Tissue lysate - whole (Zebrafish retina)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Zebrafish retina
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 03 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse brain, heart, liver, muscle, retina)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Mouse brain, heart, liver, muscle, retina
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 03 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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