Overview

  • Product name

    Anti-LDHA antibody - C-terminal
    See all LDHA primary antibodies
  • Description

    Goat polyclonal to LDHA - C-terminal
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Chimpanzee, Cynomolgus monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human LDHA aa 301-311 (C terminal) (Cysteine residue). (NP_005557.1).
    Sequence:

    C-SDLVKVTLTSE


    Database link: P00338

  • Positive control

    • WB: Human heart, skeletal muscle and kidney lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab223231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa).

Target

  • Pathway

    Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
  • Involvement in disease

    Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
  • Sequence similarities

    Belongs to the LDH/MDH superfamily. LDH family.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cell proliferation-inducing gene 19 protein antibody
    • GSD11 antibody
    • L lactate dehydrogenase A chain antibody
    • L-lactate dehydrogenase A chain antibody
    • l7R2 antibody
    • Lactate dehydrogenase 1, A chain antibody
    • Lactate dehydrogenase A antibody
    • Lactate dehydrogenase A4 antibody
    • Lactate dehydrogenase M antibody
    • LDH A antibody
    • LDH M antibody
    • LDH muscle subunit antibody
    • LDH muscle subunit; M LDH antibody
    • LDH-A antibody
    • LDH-M antibody
    • LDH1 antibody
    • ldha antibody
    • LDHA_HUMAN antibody
    • LDHM antibody
    • OTTMUSP00000017774 antibody
    • PIG19 antibody
    • Proliferation-inducing gene 19 antibody
    • Renal carcinoma antigen NY-REN-59 antibody
    see all

Images

  • All lanes : Anti-LDHA antibody - C-terminal (ab223231) at 0.3 µg/ml

    Lane 1 : Human heart lysate (in RIPA buffer)
    Lane 2 : Human skeletal muscle lysate (in RIPA buffer)
    Lane 3 : Human kidney lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 36 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

ab223231 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223231.
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