Overview

  • Product name
    Anti-LDHA antibody [EPR1564]
    See all LDHA primary antibodies
  • Description
    Rabbit monoclonal [EPR1564] to LDHA
  • Host species
    Rabbit
  • Specificity
    The immunogen for ab101562 has 93% homology with L-lactate dehydrogenase C chain (P07864).
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human LDHA aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: P00338

  • Positive control
    • HeLa, MCF-7, A375 and 293T cell lysates, Human Muscle tissue
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab101562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IHC-P 1/250 - 1/500. Antigen retrieval is strongly recommended.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Pathway
      Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
    • Involvement in disease
      Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
    • Sequence similarities
      Belongs to the LDH/MDH superfamily. LDH family.
    • Post-translational
      modifications
      ISGylated.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cell proliferation-inducing gene 19 protein antibody
      • GSD11 antibody
      • L lactate dehydrogenase A chain antibody
      • L-lactate dehydrogenase A chain antibody
      • l7R2 antibody
      • Lactate dehydrogenase 1, A chain antibody
      • Lactate dehydrogenase A antibody
      • Lactate dehydrogenase A4 antibody
      • Lactate dehydrogenase M antibody
      • LDH A antibody
      • LDH M antibody
      • LDH muscle subunit antibody
      • LDH muscle subunit; M LDH antibody
      • LDH-A antibody
      • LDH-M antibody
      • LDH1 antibody
      • ldha antibody
      • LDHA_HUMAN antibody
      • LDHM antibody
      • OTTMUSP00000017774 antibody
      • PIG19 antibody
      • Proliferation-inducing gene 19 antibody
      • Renal carcinoma antigen NY-REN-59 antibody
      see all

    Images

    • All lanes : Anti-LDHA antibody [EPR1564] (ab101562) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : A375 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 37 kDa

    • Staining of Human LDHA in a Formalin/PFA-fixed paraffin-embedded section of human Muscle using ab101526 at a dilution of 1/500.

    • Immunofluorescence analysis of rat primary oligodendrocytes, staining LDHA with ab101562.

      Cells were fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/500 in PNS) for 14 hours at 4°C. An AlexaFluor®488-conjugated donkey anti-rabbit polyclonal IgG (1/2000) was used as the secondary antibody.

      See Abreview

    • Overlay histogram showing HeLa cells stained with ab101562 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab101562, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    References

    This product has been referenced in:
    • Zhao Y  et al. MicroRNA-33b inhibits cell proliferation and glycolysis by targeting hypoxia-inducible factor-1a in malignant melanoma. Exp Ther Med 14:1299-1306 (2017). Read more (PubMed: 28781622) »
    • Cao K  et al. microRNA-33a-5p increases radiosensitivity by inhibiting glycolysis in melanoma. Oncotarget 8:83660-83672 (2017). Read more (PubMed: 29137372) »
    See all 9 Publications for this product

    Customer reviews and Q&As

    1-7 of 7 Abreviews or Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (brain lysate (lys) and myelin (mye))
    Loading amount
    15 µg
    Specification
    brain lysate (lys) and myelin (mye)
    Gel Running Conditions
    Reduced Denaturing (gel 10%)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Nov 22 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (primary Oligodendrocytes)
    Specification
    primary Oligodendrocytes
    Fixative
    Paraformaldehyde
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Nov 21 2012

    Answer

    Vielen Dank für Ihre Email.


    Ich kann Ihnen bestätigen, dass Sie einfach einen Abreview über die Homepage einsenden können. Wenn sie auf das datenblatt von ab101562 gehen gibt es direkt unter dem Produktmanen einen Link "Submit an Abreview".

    Oder Sie wählen den Reiter "Abreview" und benutzen den Link dort.

    Sie müssen sich anmelden um einen Abreview zu schrieben. Sie bekommen auch Abpoints, die dann auf Ihr Konto gutgeschrieben werden.

    Wenn Sie den Abreview ausfüllen geben Sie bitte den Gutschien Code
    in den "additional notes" an.

    Wir freuen uns auf den Abreview. Bitte lassen Sie mich wissen, falls Sie weitere Fragen haben.

    Read More

    Answer

    Thank you for your reply and for your patience.


    I have talked to the lab and we cannot guarantee that ab101018, ab85472 and ab101562 24will not cross react with other LDH family members as the sequence homology between the family members is too high to say that with absolute certainty that they will not cross react.


    Please let me know if you have any additional questions.

    Read More

    Answer

    Merci pour votre appel de hier. Je suis désolée du retard de ma réponse.

    Voici donc la liste des produits que nous avions discuté ainsi que les liens pour les protocoles que nous avons en ligne.

    Les anticorps qui ont été testé contre la protéine humaine ainsi que sur coupes paraffine:
    contre la LDH total, c'est le ab52488 et contre la LDH isoenzyme V c'est le ab101562.
    https://www.abcam.com/index.html?datasheet=52488 (or use the following: https://www.abcam.com/index.html?datasheet=52488).
    https://www.abcam.com/index.html?datasheet=101562 (or use the following: https://www.abcam.com/index.html?datasheet=101562).

    Le kit de révélation pour détecter l'anticorps primaire est le kit ab80436. Comme vous utilisez ce kit, vous n'avez pas besoin de bloquer la biotine endogène ni d'un autre agent bloquant les protéines, car il contient déjà la solution de blocage ("protein block").
    https://www.abcam.com/index.html?datasheet=80436 (or use the following: https://www.abcam.com/index.html?datasheet=80436).

    Les solutions de démasquages sont le PH 9 ab93684 pour un démasquage à pH 9 et le ab93678 pour un démasquage à pH 6.
    https://www.abcam.com/index.html?datasheet=93684 (or use the following: https://www.abcam.com/index.html?datasheet=93684).
    https://www.abcam.com/index.html?datasheet=93678 (or use the following: https://www.abcam.com/index.html?datasheet=93678).

    La solution de blocage est le ab64218 pour bloquer l'activité endogène de peroxydase. Cet étape de blocage est très important si vous travaillez avec des tissues qui contiennent de l'activité endogène de peroxydase, comme par exemple le poumon. Veuillez ne pas hésiter à nous rappeler pour discuter ceci en plus de détails.
    https://www.abcam.com/index.html?datasheet=64218 (or use the following: https://www.abcam.com/index.html?datasheet=64218).

    Voici les liens pour les protocoles d'immunohistochimie. Ils contiennent beaucoup des informations utiles.

    https://www.abcam.com/index.html?pageconfig=resource&rid=11757
    le même protocole en pdf: https://www.abcam.com/ps/pdf/protocols/ihc_p.pdf
    https://www.abcam.com/index.html?pageconfig=resource&rid=11384
    https://www.abcam.com/index.html?pageconfig=popular_protocols

    Voici aussi ici des liens plus spécifiques, comme pour le démasquage et pour les kits de détection.
    https://www.abcam.com/index.html?pageconfig=searchresults&pt=6&search=antigen retrieval
    https://www.abcam.com/index.html?pageconfig=resource&rid=12865&source=pagetrap&viapagetrap=expose

    J'espère que ces informations vous sont utiles. N'hésitez pas à nous rappeler pour plus d'information.

    Read More

    Answer

    DISCOUNT CODE: 00000

    Ablaufdatum: 06. Oktober 2012

    Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab101562 in Maus zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Sie uns ein Abreview für diesen Antikörper eingereicht haben. Der Discountcode hat die Nummer 100%ABR-E71AB, deckt den Wert von ab101562 ab und kann zum Kauf weiterer Produkte eingesetzt werden. Bitte geben Sie den Code erst bei der Bestellung andere Produktean.

    Der Code wird gültig, sobald Sie uns ein Abreview für diesen Antikörper über den Test in Maus eingereicht haben. Bitte geben Sie diesen Code auch im Abschnitt “Additional Notes” des Abreviews mit an, so dass wir wissen, dass sich dieses Abreview auf die Gutscheinaktion bezieht. Der Code wird dadurch aktiviert. Bitte kontaktieren Sie bei der Bestellung nachEinreichen des Abreviewunsere Kundendienst mit dem Code und der ursprünglichen Bestellnummer.

    Bitte zögern Sie nicht, sich wieder an uns zu wenden, falls Sie weitere Fragen haben. Wir freuen uns auf Ihr Abreview, egal ob Ihre Ergebnisse positiv oder negativ sind und wünschen Ihnen viel Glück für Ihre Forschung.

    Die Bedingungen dieses Angebotes befinden sich unter dem folgenden Link: www.abcam.com/collaborationdiscount

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    Question
    Answer

    Thank you for your phone call.
    As we discussed I am sending you the 3 discount codes for the 2 antibodies as well as the one protein, all of which have not yet been tested in sandwich ELISA.
    DISCOUNT CODE: xxx for ab85472
    DISCOUNT CODE: xxx for ab101562
    DISCOUNT CODE: xxx for ab77945
    Expiration date: xxx
    I am very pleased to hear you would like to accept our offer and test ab85472, ab101562, ab77945 in sandwich ELISA. Each code will give you either 1 free PRIMARY ANTIBODY or 1 FREE PROTEIN before the expiration date. To redeem this offer, please submit an Abreview for sandwich ELISA and include the codes in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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