Anti-LDHA antibody [EPR1564] (ab101562)


  • Product name
    Anti-LDHA antibody [EPR1564]
    See all LDHA primary antibodies
  • Description
    Rabbit monoclonal [EPR1564] to LDHA
  • Host species
  • Specificity
    The immunogen for ab101562 has 93% homology with L-lactate dehydrogenase C chain (P07864).
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human LDHA aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: P00338

  • Positive control
    • HeLa, MCF-7, A375 and 293T cell lysates, Human Muscle tissue
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab101562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IHC-P 1/250 - 1/500. Antigen retrieval is strongly recommended.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Pathway
      Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
    • Involvement in disease
      Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
    • Sequence similarities
      Belongs to the LDH/MDH superfamily. LDH family.
    • Post-translational
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • Cell proliferation-inducing gene 19 protein antibody
      • GSD11 antibody
      • L lactate dehydrogenase A chain antibody
      • L-lactate dehydrogenase A chain antibody
      • l7R2 antibody
      • Lactate dehydrogenase 1, A chain antibody
      • Lactate dehydrogenase A antibody
      • Lactate dehydrogenase A4 antibody
      • Lactate dehydrogenase M antibody
      • LDH A antibody
      • LDH M antibody
      • LDH muscle subunit antibody
      • LDH muscle subunit; M LDH antibody
      • LDH-A antibody
      • LDH-M antibody
      • LDH1 antibody
      • ldha antibody
      • LDHA_HUMAN antibody
      • LDHM antibody
      • OTTMUSP00000017774 antibody
      • PIG19 antibody
      • Proliferation-inducing gene 19 antibody
      • Renal carcinoma antigen NY-REN-59 antibody
      see all


    • All lanes : Anti-LDHA antibody [EPR1564] (ab101562) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : A375 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 37 kDa

    • Staining of Human LDHA in a Formalin/PFA-fixed paraffin-embedded section of human Muscle using ab101526 at a dilution of 1/500.

    • Immunofluorescence analysis of rat primary oligodendrocytes, staining LDHA with ab101562.

      Cells were fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/500 in PNS) for 14 hours at 4°C. An AlexaFluor®488-conjugated donkey anti-rabbit polyclonal IgG (1/2000) was used as the secondary antibody.

      See Abreview

    • Overlay histogram showing HeLa cells stained with ab101562 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab101562, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.


    This product has been referenced in:
    • Zhao Y  et al. MicroRNA-33b inhibits cell proliferation and glycolysis by targeting hypoxia-inducible factor-1a in malignant melanoma. Exp Ther Med 14:1299-1306 (2017). Read more (PubMed: 28781622) »
    • Cao K  et al. microRNA-33a-5p increases radiosensitivity by inhibiting glycolysis in melanoma. Oncotarget 8:83660-83672 (2017). Read more (PubMed: 29137372) »

    See all 8 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Western blot
    Mouse Tissue lysate - whole (brain lysate (lys) and myelin (mye))
    Loading amount
    15 µg
    brain lysate (lys) and myelin (mye)
    Gel Running Conditions
    Reduced Denaturing (gel 10%)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Nov 22 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Immunocytochemistry/ Immunofluorescence
    Rat Cell (primary Oligodendrocytes)
    primary Oligodendrocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Nov 21 2012

    Vielen Dank für Ihre Email.

    Ich kann Ihnen bestätigen, dass Sie einfach einen Abreview über die Homepage einsenden können. Wenn sie auf das datenblatt von ab101562 gehen gibt es direkt unter dem Produktmanen einen Lin...

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    Thank you for your reply and for your patience.

    I have talked to the lab and we cannot guarantee that ab101018, ab85472 and ab101562 24will not cross react with other LDH family members as the sequence homology between the family members ...

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    Merci pour votre appel de hier. Je suis désolée du retard de ma réponse.

    Voici donc la liste des produits que nous avions discuté ainsi que les liens pour les protocoles que nous avons en ligne.

    Les antic...

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    DISCOUNT CODE: 00000

    Ablaufdatum: 06. Oktober 2012

    Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab101562 in Maus zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutsche...

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    Thank you for your phone call.
    As we discussed I am sending you the 3 discount codes for the 2 antibodies as well as the one protein, all of which have not yet been tested in sandwich ELISA.
    DISCOUNT CODE: xxx for ab85472

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