Product nameAnti-LDHA antibody [EPR1564]
See all LDHA primary antibodies
DescriptionRabbit monoclonal [EPR1564] to LDHA
SpecificityThe immunogen for ab101562 has 93% homology with L-lactate dehydrogenase C chain (P07864).
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
within Human LDHA aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: P00338
- HeLa, MCF-7, A375 and 293T cell lysates, Human Muscle tissue
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab101562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 37 kDa.|
|IHC-P||1/250 - 1/500. Antigen retrieval is strongly recommended.|
|ICC/IF||1/100 - 1/250.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
PathwayFermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Involvement in diseaseDefects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
Sequence similaritiesBelongs to the LDH/MDH superfamily. LDH family.
- Information by UniProt
- Cell proliferation-inducing gene 19 protein antibody
- GSD11 antibody
- L lactate dehydrogenase A chain antibody
All lanes : Anti-LDHA antibody [EPR1564] (ab101562) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : A375 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 37 kDa
Staining of Human LDHA in a Formalin/PFA-fixed paraffin-embedded section of human Muscle using ab101526 at a dilution of 1/500.
Immunofluorescence analysis of rat primary oligodendrocytes, staining LDHA with ab101562.
Cells were fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/500 in PNS) for 14 hours at 4°C. An AlexaFluor®488-conjugated donkey anti-rabbit polyclonal IgG (1/2000) was used as the secondary antibody.
Overlay histogram showing HeLa cells stained with ab101562 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab101562, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This product has been referenced in:
- Zhao Y et al. MicroRNA-33b inhibits cell proliferation and glycolysis by targeting hypoxia-inducible factor-1a in malignant melanoma. Exp Ther Med 14:1299-1306 (2017). Read more (PubMed: 28781622) »
- Cao K et al. microRNA-33a-5p increases radiosensitivity by inhibiting glycolysis in melanoma. Oncotarget 8:83660-83672 (2017). Read more (PubMed: 29137372) »