Recombinant
RabMAb

Recombinant Anti-LDHA antibody [EPR1564] - BSA and Azide free (ab226016)

Overview

  • Product name

    Anti-LDHA antibody [EPR1564] - BSA and Azide free
    See all LDHA primary antibodies
  • Description

    Rabbit monoclonal [EPR1564] to LDHA - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human LDHA aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: P00338

  • General notes

    Ab226016 is the carrier-free version of ab101562. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab226016 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab226016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is strongly recommended.

WB Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Pathway

      Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
    • Involvement in disease

      Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
    • Sequence similarities

      Belongs to the LDH/MDH superfamily. LDH family.
    • Post-translational
      modifications

      ISGylated.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cell proliferation-inducing gene 19 protein antibody
      • GSD11 antibody
      • L lactate dehydrogenase A chain antibody
      • L-lactate dehydrogenase A chain antibody
      • l7R2 antibody
      • Lactate dehydrogenase 1, A chain antibody
      • Lactate dehydrogenase A antibody
      • Lactate dehydrogenase A4 antibody
      • Lactate dehydrogenase M antibody
      • LDH A antibody
      • LDH M antibody
      • LDH muscle subunit antibody
      • LDH muscle subunit; M LDH antibody
      • LDH-A antibody
      • LDH-M antibody
      • LDH1 antibody
      • ldha antibody
      • LDHA_HUMAN antibody
      • LDHM antibody
      • OTTMUSP00000017774 antibody
      • PIG19 antibody
      • Proliferation-inducing gene 19 antibody
      • Renal carcinoma antigen NY-REN-59 antibody
      see all

    Images

    • Staining of Human LDHA in a Formalin/PFA-fixed paraffin-embedded section of human Muscle using ab101526 at a dilution of 1/500.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab101562).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    ab226016 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab226016.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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