Recombinant Anti-LDHD/DLD antibody [EPR15068(B)] (ab182146)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15068(B)] to LDHD/DLD
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-LDHD/DLD antibody [EPR15068(B)]
See all LDHD/DLD primary antibodies -
Description
Rabbit monoclonal [EPR15068(B)] to LDHD/DLD -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal liver and fetal heart lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR15068(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 55 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 55 kDa). |
Target
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Tissue specificity
Expressed moderately in heart and liver and at lower levels in skeletal muscle and kidney. -
Sequence similarities
Belongs to the FAD-binding oxidoreductase/transferase type 4 family.
Contains 1 FAD-binding PCMH-type domain. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 197257 Human
- Entrez Gene: 52815 Mouse
- Omim: 607490 Human
- SwissProt: Q86WU2 Human
- SwissProt: Q7TNG8 Mouse
- Unigene: 380929 Human
- Unigene: 271578 Mouse
- Unigene: 27589 Mouse
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Alternative names
- D lactate dehydrogenase antibody
- DLD antibody
- Lactate dehydrogenase D antibody
see all
Images
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All lanes : Anti-LDHD/DLD antibody [EPR15068(B)] (ab182146) at 1/2000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 55 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab182146 has not yet been referenced specifically in any publications.