Overview

  • Product name
    Anti-LDL (MDA oxidized) antibody [4C7]
    See all LDL (MDA oxidized) primary antibodies
  • Description
    Mouse monoclonal [4C7] to LDL (MDA oxidized)
  • Host species
    Mouse
  • Tested applications
    Suitable for: ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Low-density lipoprotein purified from human plasma and oxidized with malondialdehyde (MDA).

Properties

Applications

Our Abpromise guarantee covers the use of ab17354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution.
    Immunoblotting: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Low-density lipoprotein (LDL) is the carrier protein for cholesterol in the blood. LDL binds to its receptor on the capillary walls and thereby mediates the uptake and clearence of cholesterol from the circulation. In atherosclerotic lesions oxidatively modified LDL is found and oxidized LDL is specifically recognized and ingested by macrophages via scavenger receptor A and CD36. Oxidized LDL may be a marker of atherosclerosis but the precise changes in oxidized LDL are not well described. MDA-oxidized LDL appear to be different from LDL oxidized by other means.
    • Cellular localization
      Secreted
    • Alternative names
      • Low density lipoprotein antibody
      • MDA oxidized LDL antibody
      • MDA oxidized low density lipoprotein antibody

    References

    ab17354 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer

    Thank you for getting back to me and explaining these issues to me. I am convinced that there is a problem with this antibody in the system that you are using. I would like to offer you a credit note provided the antibody was purchased within the past 90 days. If this is the case, please can you e-mail me details of the date of purchase and the purchase order number and I will arrange for our accounts department to raise a credit note for you. I look forward to hearing from you.

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    Answer

    Thank you for your enquiry. I have been in touch with the source of this antibody and they have provided me with the following information. "I am afraid that only MDA (malon di aldehyde) oxidized LDL will work, as mentioned in the product specification. We expect the epitope to be a MDA-lysine residue, though this is just theory. I am not familiar with AAPH, but apparently it yields another epitope". They suggest that chemical oxidation by AAPH yields an epitope that cannot be recognized by the antibody. Please can you comment on whether you consider this a possibility; as this may explain the absence of signal? I look forward to hearing from you.

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    Answer

    Thank you for your enquiry. Yes, this antibody reacts with oxidized LDL but not with native LDL. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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